|本期目录/Table of Contents|

[1]王喜英,赵辉,谭智勇,等.设施菜地种植年限对土壤nosZ型反硝化微生物群落结构和丰度的影响[J].江苏农业科学,2022,50(9):240-247.
　Wang Xiying,et al.Effects of cultivation years in protected vegetable crop fields on structure and abundance of soil nosZ denitrifying microbial community[J].Jiangsu Agricultural Sciences,2022,50(9):240-247.
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设施菜地种植年限对土壤nosZ型反硝化微生物群落结构和丰度的影响(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第50卷
期数:
2022年第9期
页码:
240-247
栏目:
资源与环境
出版日期:
2022-05-05

文章信息/Info

Title:
Effects of cultivation years in protected vegetable crop fields on structure and abundance of soil nosZ denitrifying microbial community
作者:
王喜英; 赵辉; 谭智勇; 余高
铜仁学院，贵州铜仁 554300
Author(s):
Wang Xiying; et al
关键词:
设施菜地; 种植年限; 反硝化微生物; nosZ基因; 群落结构
Keywords:
-
分类号:
S182
DOI:
-
文献标志码:
A
摘要:
反硝化微生物在土壤氮素损失和温室气体转化方面具有重要的作用，研究设施菜地土壤反硝化微生物群落结构和数量变化，对评价设施菜地长期种植土壤质量状况和提高氮转化认知水平具有重要意义。应用荧光定量PCR和 Illumina Miseq高通量测序技术，以nosZ基因为靶标，研究设施菜地种植3、5、7年和露天菜地(CK)对土壤反硝化微生物群落结构和数量的影响。结果表明：露天菜地(CK)nosZ基因丰度显著高于其他处理，分别是3、5、7年的1.32倍、1.45倍和1.69倍。随种植年限延长，nosZ基因丰度逐渐降低。不同处理土壤反硝化微生物群落α多样性指数差异显著，α多样性指数随种植年限延长逐渐降低，且露天菜地(CK)的Chao1指数和ACE指数最高。门水平上优势类群为变形菌门；属水平上优势类群为慢生根瘤菌属和无色杆菌属；变形菌门和慢生根瘤菌属相对丰度随设施种植年限延长逐渐降低。主成分分析(PCA)结果表明，随种植年限延长nosZ群落结构差异较大；其中3年和5 年群落结构相似，7、3、5 年群落结构差异较大。土壤速效钾、铵态氮和硝态氮含量是nosZ型反硝化微生物数量、α多样性和群落结构的主要影响因素。综上可知，设施菜地长期种植显著降低了nosZ型反硝化微生物数量，并对群落结构有显著影响。
Abstract:
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备注/Memo

备注/Memo:
收稿日期：2021-08-01
基金项目：贵州省教育厅自然科学研究项目(编号：黔教合KY字［2020］163)；铜仁学院博士科研启动基金(编号：tyxyDH2002)；铜仁学院农业生态创新研究项目(编号：CXTD［2020-10］)；绿色农药与农业生物工程教育部重点实验室开放基金(编号：黔教合KY字［2019］036)；贵州省教育厅青年科技人才成长项目(编号：黔教合KY字［2019］175)。
作者简介：王喜英(1981—)，女，河南汝南人，硕士，讲师，研究方向为生物化学及生物信息学分
更新日期/Last Update: 2022-05-05