|本期目录/Table of Contents|

[1]封丽梅,林淼,姜茂成,等.电子供体对向日葵秸秆厌氧发酵产酸和微生物群落结构的影响[J].江苏农业科学,2022,50(9):247-252.
 Feng Limei,et al.Impacts of electronic donors on acid yield and microbial community structure of anaerobic fermenting with sunflower straw[J].Jiangsu Agricultural Sciences,2022,50(9):247-252.
点击复制

电子供体对向日葵秸秆厌氧发酵产酸和微生物群落结构的影响(PDF)
分享到:

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第50卷
期数:
2022年第9期
页码:
247-252
栏目:
资源与环境
出版日期:
2022-05-05

文章信息/Info

Title:
Impacts of electronic donors on acid yield and microbial community structure of anaerobic fermenting with sunflower straw
作者:
封丽梅林淼姜茂成程秀花吉慧敏
扬州大学动物科学与技术学院,江苏扬州 225009
Author(s):
Feng Limeiet al
关键词:
乙醇乳酸瘤胃微生物向日葵秸秆C2~C6脂肪酸
Keywords:
-
分类号:
X712
DOI:
-
文献标志码:
A
摘要:
为探究电子供体对瘤胃微生物发酵向日葵秸秆产C2~C6脂肪酸的影响,采用体外连续传代和高通量测序技术,比较添加乙醇或乳酸对脂肪酸产量及细菌和真菌群落结构的影响。结果表明,添加乙醇和乳酸显著提高了总C2~C6脂肪酸的产量,乙醇提高了乙酸、戊酸和己酸产量,乳酸提高了丙酸、丁酸、戊酸和己酸产量。与对照组相比,乙醇组的拟杆菌门相对丰度下降,而变形菌门相对丰度上升;乳酸组的变形菌门相对丰度下降,而放线菌门相对丰度上升。添加乙醇或乳酸对相对丰度前5的真菌菌门无显著影响。添加2种电子供体都显著改变了细菌和真菌的群落结构。添加乙醇显著提高萨特氏菌属、解琥珀酸菌属和脱硫弧菌属的相对丰度,添加乳酸显著提高巨型球菌属、Shuttleworthia、互营球菌属、光冈菌属、未定义的普雷沃氏菌的相对丰度。在种水平上,普雷沃氏菌、亨氏丁酸弧菌、埃氏巨型球菌与丁酸、戊酸产量呈显著相关。
Abstract:
-

参考文献/References:

[1]石祖梁,王飞,王久臣,等. 我国农作物秸秆资源利用特征、技术模式及发展建议[J]. 中国农业科技导报,2019,21(5):8-16.
[2]Sharma R. Agricultural straw burning and its impact on the environment[J]. International Journal of Environment Research,2021,3(7):26-31.
[3]Kumar D,Singh B,Korstad J. Utilization of lignocellulosic biomass by oleaginous yeast and bacteria for production of biodiesel and renewable diesel[J]. Renewable and Sustainable Energy Reviews,2017,73:654-671.
[4]Tsui T H,Wong J W C. A critical review:emerging bioeconomy and waste-to-energy technologies for sustainable municipal solid waste management[J]. Waste Disposal & Sustainable Energy,2019(3):151-167.
[5]Sherif N,Gadalla M,Kamel D. Acid-hydrolysed furfural production from rice straw bio-waste:process synthesis,simulation,and optimisation[J]. South African Journal of Chemical Engineering,2021,38:34-40.
[6]Poszytek K,Ciezkowska M,Sklodowska A,et al. Microbial consortium with high cellulolytic activity (MCHCA) for enhanced biogas production[J]. Frontiers in Microbiology,2016,7:324.
[7]Safaripour M,Ghanbari A,Seyedabadi E,et al. Investigation of environmental impacts of bioethanol production from wheat straw in Kermanshah,Iran[J]. Biomass Conversion and Biorefinery,2021:1-11.
[8]林淼,王阔鹏,陈映良,等. 乙醇对瘤胃液接种稻秸的体外发酵产物及细菌群落结构的影响[J]. 生物技术通报,2020,36(2):91-99.
[9]Patel A K,Singhania R R,Sim S J,et al. Thermostable cellulases:current status and perspectives[J]. Bioresource Technology,2019,279:385-392.
[10]Zheng Y,Zhao J,Xu F Q,et al. Pretreatment of lignocellulosic biomass for enhanced biogas production[J]. Progress in Energy and Combustion Science,2014,42:35-53.
[11]田亚红,王巍杰,王丽萍,等. 向日葵秸秆发酵生产乙醇工艺的研究[J]. 酿酒科技,2013(6):40-42.
[12]张佳,王园,安晓萍,等. 向日葵副产物的营养特性及在反刍动物中的应用[J]. 中国畜牧兽医,2021,48(3):916-924.
[13]Yue Z B,Li W W,Yu H Q. Application of rumen microorganisms for anaerobic bioconversion of lignocellulosic biomass[J]. Bioresource Technology,2013,128:738-744.
[14]艾平,田启欢,席江,等. 稻秸预处理厌氧强化产挥发性脂肪酸研究[J]. 农业机械学报,2018,49(3):309-316.
[15]Bengtsson S,Hallquist J,Werker A,et al. Acidogenic fermentation of industrial wastewaters:effects of chemostat retention time and pH on volatile fatty acids production[J]. Biochemical Engineering Journal,2008,40(3):492-499.
[16]Weimer P J,Kohn R A. Impacts of ruminal microorganisms on the production of fuels:how can we intercede from the outside?[J]. Applied Microbiology and Biotechnology,2016,100(8):3389-3398.
[17]Grootscholten T I M,Steinbusch K J J,Hamelers H V M,et al. Chain elongation of acetate and ethanol in an upflow anaerobic filter for high rate MCFA production[J]. Bioresource Technology,2013,135:440-445.
[18]王薪淯,朱晓宇,李海翔,等. 乳酸碳链延长技术及其在有机废弃物资源化中的应用研究进展[J]. 应用与环境生物学报,2020,26(4):827-835.
[19]Lin M,Dai X X,Weimer P J. Shifts in fermentation end products and bacterial community composition in long-term,sequentially transferred in vitro ruminal enrichment cultures fed switchgrass with and without ethanol as a co-substrate[J]. Bioresource Technology,2019,285:121324.
[20]Chen W S,Strik D P B T B,Buisman C J N,et al. Production of caproic acid from mixed organic waste:an environmental life cycle perspective[J]. Environmental Science & Technology,2017,51(12):7159-7168.
[21]Lin M,Feng L M,Cheng Z Q,et al. Effect of ethanol or lactic acid on volatile fatty acid profile and microbial community in short-term sequentially transfers by ruminal fermented with wheat straw in vitro[J]. Process Biochemistry,2021,102:369-375.
[22]Kleen J L,Hooijer G A,Rehage J,et al. Subacute ruminal acidosis (SARA):a review[J]. Journal of Veterinary Medicine Series A,2003,50(8):406-414.
[23]Chwialkowska J,Duber A,Zagrodnik R,et al. Caproic acid production from acid whey via open culture fermentation—evaluation of the role of electron donors and downstream processing[J]. Bioresource Technology,2019,279:74-83.
[24]Weimer P J,da Silva C L,Cacite F. Effects of ruminal dosing of Holstein cows with Megasphaera elsdenii on milk fat production,ruminal chemistry,and bacterial strain persistence[J]. Journal of Dairy Science,2015,98(11):8078-8092.
[25]Angenent L T,Richter H,Buckel W,et al. Chain elongation with reactor microbiomes:open-culture biotechnology to produce biochemicals[J]. Environmental Science & Technology,2016,50(6):2796-2810.
[26]Weimer P J,Moen G N. Quantitative analysis of growth and volatile fatty acid production by the anaerobic ruminal bacterium Megasphaera elsdenii T81[J]. Applied Microbiology and Biotechnology,2013,97(9):4075-4081.
[27]Kim H,Choi O,Jeon B S,et al. Impact of feedstocks and downstream processing technologies on the economics of caproic acid production in fermentation by Megasphaera elsdenii T81[J]. Bioresource Technology,2020,301:122794.
[28]张洁,张力莉,徐晓锋. 反刍动物瘤胃内普雷沃氏菌的研究进展[J]. 中国饲料,2020(7):17-21.
[29]Fraga M,Fernández S,Perelmuter K,et al. The use of Prevotella bryantii 3C5 for modulation of the ruminal environment in an ovine model[J]. Brazilian Journal of Microbiology,2018,49:101-106.
[30]Yang P X,Leng L,Tan G Y A,et al. Upgrading lignocellulosic ethanol for caproate production via chain elongation fermentation[J]. International Biodeterioration & Biodegradation,2018,135:103-109.
[31]Solomon K V,Haitjema C H,Henske J K,et al. Early-branching gut fungi possess a large,comprehensive array of biomass-degrading enzymes[J]. Science,2016,351(6278):1192-1195.
[32]Sun X H,Gostincˇar C,Fang C,et al. Genomic evidence of recombination in the basidiomycete Wallemia mellicola[J]. Genes,2019,10(6):427.
[33]Skalski J H,Limon J J,Sharma P,et al. Expansion of commensal fungus Wallemia mellicola in the gastrointestinal mycobiota enhances the severity of allergic airway disease in mice[J]. PLoS Pathogens,2018,14(9):e1007260.

相似文献/References:

[1]张宏喜,周婷婷,李楠,等.利用乙醇自催化法提取棉秆中的木质素[J].江苏农业科学,2013,41(06):243.
 Zhang Hongxi,et al.Extraction of lignin from cotton stalks by pulping autocatalytic method of ethanol[J].Jiangsu Agricultural Sciences,2013,41(9):243.
[2]胡耀池,章文贵,詹妮娜,等.乙醇脱水产物的气相色谱分析[J].江苏农业科学,2014,42(10):286.
 Hu Yaochi,et al.Analysis of ethanol dehydration products via gas chromatography[J].Jiangsu Agricultural Sciences,2014,42(9):286.
[3]牟建梅,张国芹,刘凤军,等.白菜叶绿素含量的测定方法筛选[J].江苏农业科学,2014,42(09):289.
 Mou Jianmei,et al.Screening of determination methods of chlorophyll content of non heading Chinese cabbage[J].Jiangsu Agricultural Sciences,2014,42(9):289.
[4]王豪,徐致远,刘振民,等.不同发酵温度对开菲尔产氨基酸及理化性质的影响[J].江苏农业科学,2014,42(09):236.
 Wang Hao,et al.Effects of different fermentation temperatures on amino acid production and physicochemical properties of kefir[J].Jiangsu Agricultural Sciences,2014,42(9):236.
[5]张印,郭建凤,蔺海朝,等.不同储藏条件下长白猪和大约克猪肌肉pH值、TBA值、糖原及乳酸含量比较[J].江苏农业科学,2016,44(06):361.
 Zhang Yin,et al.Comparison of muscle pH value,TBA value,glycogen contents,and lactic acid contents of Landrace and Yorkshire under different storage conditions[J].Jiangsu Agricultural Sciences,2016,44(9):361.
[6]陈俊英,刘永丽,黄会杰,等.木薯粉乙醇清液发酵中糖化条件的研究[J].江苏农业科学,2015,43(07):272.
 Chen Junying,et al.Study on saccharification condition of ethanol fermentation of cassava starch clarifying liquor[J].Jiangsu Agricultural Sciences,2015,43(9):272.
[7]高慧,陈燕.江苏省淮安市设施土壤根结线虫发生状况及乙醇防治效果[J].江苏农业科学,2014,42(07):131.
 Gao Hui,et al.Incidence and ethanol control effect of root knot nematodes in greenhouse soil in Huaian,Jiangsu Province[J].Jiangsu Agricultural Sciences,2014,42(9):131.
[8]费文斌,雍晓雨,徐俊,等.1株耐热产乙醇酵母的分离、鉴定与性能测试[J].江苏农业科学,2015,43(03):319.
 Fei Wenbin,et al.Separation,identification and performance test of a strain of heat-resisting and alcohol producing yeast[J].Jiangsu Agricultural Sciences,2015,43(9):319.
[9]乐易林,倪黎,郭星星,等.嗜热菌乙醇代谢途径研究进展[J].江苏农业科学,2016,44(11):22.
 Le Yilin,et al.Research progress of ethanol metabolic pathway of thermophilic microorganisms[J].Jiangsu Agricultural Sciences,2016,44(9):22.
[10]刘媛,戚露月,顾伟光,等.麦苗运动饮料抗疲劳功能研究[J].江苏农业科学,2018,46(19):216.
 Liu Yuan,et al.Study on anti-fatigue function of sport drinks with wheat seedlings[J].Jiangsu Agricultural Sciences,2018,46(9):216.

备注/Memo

备注/Memo:
收稿日期:2021-12-30
基金项目:扬州大学研究生科研与实践创新计划(编号:XSJCX20_032);国家现代农业产业技术体系建设专项;江苏省高校优势学科建设工程。
作者简介:封丽梅(1996—),女,江苏盐城人,硕士研究生,主要从事瘤胃挥发性脂肪酸的功能及代谢研究。E-mail:fenglimei1996@163.com。
通信作者:林淼,博士,副教授,主要从事瘤胃微生态及分子营养研究。E-mail:linmiao@yzu.edu.cn。
更新日期/Last Update: 2022-05-05