|本期目录/Table of Contents|

[1]杜娟,申李旖琦,聂丽妍,等.西藏青稞穗腐病病原菌鉴定与室内药剂筛选[J].江苏农业科学,2022,50(14):108-114.
 Du Juan,et al.Identification of pathogenic bacteria of ear rot of highland barley in Tibet and screening of indoor chemicals[J].Jiangsu Agricultural Sciences,2022,50(14):108-114.
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西藏青稞穗腐病病原菌鉴定与室内药剂筛选(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第50卷
期数:
2022年第14期
页码:
108-114
栏目:
植物保护
出版日期:
2022-07-20

文章信息/Info

Title:
Identification of pathogenic bacteria of ear rot of highland barley in Tibet and screening of indoor chemicals
作者:
杜娟1申李旖琦1聂丽妍2魏丽萍2孙玉1巩文峰1
1.西藏农牧学院植物科学学院,西藏林芝 860000; 2.资源与环境学院,西藏林芝 860000
Author(s):
Du Juanet al
关键词:
青稞穗腐病鉴定生物学特性毒力测定
Keywords:
-
分类号:
S435.123
DOI:
-
文献标志码:
A
摘要:
为明确西藏自治区山南隆子和日喀则江孜青稞穗腐病病原种类、生物学特性以及防治药剂,以期为青稞穗腐病的综合防控提供一定参考,从病害发生区采集病穗样本,分离获得病原菌纯培养,利用依据分生孢子、分支及菌落形态做形态学特性,再结合内转录间隔区(internal transcribed spacer,简称ITS)和转录延伸因子(translation elongation factor 1α,简称EF-1α)进行分子鉴定;对病原物的生物学特性进行研究,并选用6种常见农药对病原菌进行室内药剂筛选研究。结果表明,根据菌落形态、分生孢子形态、ITS和EF-1α序列鉴定,该穗腐病原菌为燕麦镰刀菌(Fusarium avenaceum)。在隆子采集的病原菌最适宜生长的碳源是葡萄糖和麦芽糖,氮源是硝酸钾;在江孜采集的病原菌最适宜生长的碳源是淀粉,氮源是蛋白胨。药剂筛选和毒力测定结果表明苯醚甲环唑是本试验中最佳防治药剂,其EC50为1.270 9 mg/L。引起西藏隆子县和江孜县穗腐病的主要病原菌为燕麦镰刀菌,且不同地点病原菌间的生物学特性存在不同,推荐使用防治杀菌剂是苯醚甲环唑。
Abstract:
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参考文献/References:

[1]岳海梅,庄华,巩文峰,等. 青稞全蚀病和根腐病生防毛壳菌的筛选及鉴定[J]. 麦类作物学报,2017,37(10):1390-1397.
[2]徐飞,王俊美,杨共强,等. 河南省首次发现梨孢镰刀菌引起的小麦赤霉病[J]. 植物保护,2020,46(6):60-64.
[3]赵娜,杜秀明,李令蕊,等. 我国小麦赤霉病发生与控制研究进展[J]. 河北农业科学,2020,24(2):54-58.
[4]陈文华,殷宪超,武德亮,等. 小麦赤霉病生物防治研究进展[J]. 江苏农业科学,2020,48(4):12-18.
[5]陈琳,蔺瑞明,王凤涛,等. 禾生指葡孢霉的遗传多样性及对苗期青稞的致病性[J]. 中国农业科学,2020,53(1):213-224.
[6]孙华,李坡,郭宁,等. 玉米穗腐病样本中温和镰孢菌的鉴定及其生物学特性[J]. 玉米科学,2020,28(1):177-183,190.
[7]Leslie J F,Summerell B A . The Fusarium laboratory manual[M]. AMes:Blackwell Pub Professional,2006:111-119.
[8]胡颂平,余建,魏开发,等. 江西水稻穗腐病病原菌鉴定及生物学特性研究[J]. 江西农业大学学报,2019,41(2):234-242.
[9]周佳,王铁霖,苗玉焕,等. 乌头黑斑病病原菌的鉴定、生物学特性及其有效杀菌剂研究[J]. 中国中药杂志,2022,47(5):1215-1221.
[10]易铭,梁嘉俊,史建,等. 采用EF-1α序列分析法对苜蓿根腐病病原菌:锐顶镰刀菌的鉴定[J]. 草业学报,2017,26(2):61-68.
[11]秦子惠,任旭,江凯,等. 我国玉米穗腐病致病镰孢种群及禾谷镰孢复合种的鉴定[J]. 植物保护学报,2014,41(5):589-596.
[12]瞿佳,门欣,孙晓宇,等. 陕西核桃黑斑病病原菌鉴定及药剂防治研究[J]. 西北农业学报,2021,30(3):452-461.
[13]宁楠楠,刘俏,咸文荣,等. 5种杀菌剂对马铃薯干腐病菌的室内毒力测定[J]. 青海大学学报,2021,39(1):31-37.
[14]龚弘强,黄丽丽,乔宏萍,等. 西藏青稞籽粒燕麦镰刀菌生物学特性研究[J]. 干旱地区农业研究,2007,25(2):21-25.
[15]辛秀竹,刘豆豆,石怡彤,等. 禾谷镰刀菌对小麦种子萌发及幼苗形态的影响[J]. 河北农业科学,2021,25(1):66-69.
[16]卢良恕. 中国大麦学[M]. 北京:中国农业出版社,1996.
[17]中国作物学会大麦专业委员会. 中国大麦文集[M]. 北京:中国农业科技出版社,1986.
[18]Satapute P,Kamble M V,Adhikari S S,et al. Influence of triazole pesticides on tillage soil microbial populations and metabolic changes[J]. Science of the Total Environment,2019,651:2334-2344.
[19]Kumar S,Kashyap P L,Saharan M S,et al. Difenoconazole:a new seed dressing molecule for effective management of flag smut (Urocystis agropyri) of wheat[J]. J Cereal Res,2019,11(1):37-40.
[20]Shcherbakova L,Mikityuk O,Arslanova L,et al. Studying the ability of thymol to improve fungicidal effects of tebuconazole and difenoconazole against some plant pathogenic fungi in seed or foliar treatments[J]. Frontiers in Microbiology,2021,12:629429.
[21]Hester S,Moore T,Padgett W T,et al. The hepatocarcinogenic conazoles:cyproconazole,epoxiconazole,and propiconazole induce a common set of toxicological and transcriptional responses[J]. Toxicological Sciences,2012,127(1):54-65.
[22]Wang X,Ni H F,Xu W P,et al. Difenoconazole induces oxidative DNA damage and mitochondria mediated apoptosis in SH-SY5Y cells[J]. Chemosphere,2021,283:131160.
[23]Chatterjee N,Walker G C. Mechanisms of DNA damage,repair,and mutagenesis[J]. Environmental and Molecular Mutagenesis,2017,58(5):235-263.
[24]Friedberg E C. DNA damage and repair[J]. Nature,2003,421(6921):436-440.
[25]Harper J W,Elledge S J. The DNA damage response:ten years after[J]. Molecular Cell,2007,28(5):739-745.
[26]Su M,Wang H R,Wang W X,et al. LncRNAs in DNA damage response and repair in cancer cells[J]. Acta Biochimica et Biophysica Sinica,2018,50(5):433-439.

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备注/Memo

备注/Memo:
收稿日期:2021-11-17
基金项目:国家自然科学基金(编号:31960002)。
作者简介:杜娟(1997—),女,四川万源人,硕士研究生,从事生物防治与微生物利用方面的研究。E-mail:1663534551@qq.com。
通信作者:巩文峰,硕士,副教授,从事生物防治与微生物资源利用。E-mail:zkxygwf@xza.edu.cn。
更新日期/Last Update: 2022-07-20