|本期目录/Table of Contents|

[1]李青,葛传龙,欧阳力剑,等.贵州草海鲫鱼HIF-1α和HIF-2α基因克隆与表达分析[J].江苏农业科学,2022,50(17):31-38.
　Li Qing,et al.Cloning and expression analysis of HIF-1α and HIF-2α inCarassius auratus of Caohai Wetland， Guizhou Province[J].Jiangsu Agricultural Sciences,2022,50(17):31-38.
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贵州草海鲫鱼HIF-1α和HIF-2α基因克隆与表达分析(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第50卷
期数:
2022年第17期
页码:
31-38
栏目:
生物技术
出版日期:
2022-09-05

文章信息/Info

Title:
Cloning and expression analysis of HIF-1α and HIF-2α inCarassius auratus of Caohai Wetland， Guizhou Province
作者:
李青1; 2; 葛传龙1; 2; 欧阳力剑1; 2; 金志梅2; 杨钦2
1.贵州省典型高原湿地生态保护与修复重点实验室，贵州毕节 551700； 2.贵州工程应用技术学院生态工程学院，贵州毕节 551700
Author(s):
Li Qing; et al
关键词:
鲫鱼; HIF1α基因; HIF2α基因; 组织表达; 生物学功能
Keywords:
-
分类号:
S917
DOI:
-
文献标志码:
A
摘要:
为研究鲫鱼中HIF不同亚型基因的结构与生物学功能，克隆获得鲫鱼中HIF同源基因的2种亚型，分别命名为Ca-HIF1α和Ca-HIF2α。其中，Ca-HIF1α cDNA全长共3 867 bp，开放阅读框2 322 bp，编码774个氨基酸。Ca-HIF2α cDNA全长共4 647 bp，开放阅读框2 460 bp，编码820个氨基酸。预测蛋白质的分子质量分别为85.844、91.228 ku，等电点分别为5.12和6.20。SMART分析结果可知，二者具有HLH、PAS和PAC结构域，此外，多序列比对结果显示二者还具有ODD、N-TAD和C-TAD功能域。进化树结构表明，Ca-HIF2α比Ca-HIF1α进化得要快。实时荧光定量PCR结果显示，Ca-HIF2α mRNA在所检测组织中的相对表达量均高于Ca-HIF1α mRNA的表达量。此外，Ca-HIF1α和Ca-HIF2α mRNA在肝脏和鳃的相对表达量最高。
Abstract:
-

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备注/Memo

备注/Memo:
收稿日期：2021-10-19
基金项目：贵州省科技计划项目(编号：黔科合基础［2018］1054)；毕科人才合字［2021］11号；毕节市六冲河流域生物保护与生态修复人才团队。
作者简介：李青(1989—)，女，山西临汾人，博士，副教授，主要从事水生动物生殖发育方面的研究。E-mail：liqingdream@163.com。
更新日期/Last Update: 2022-09-05