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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:

第50卷

期数:

2022年第18期

页码:

60-65

栏目:

基因组学与功能基因

出版日期:

2022-09-20

文章信息/Info

Title:

Cloning and salt stress response analysis of CsNAC032 gene in cucumber

作者:

李宝昌¹; 陈岳¹; 2; 张涵¹; 张微微¹

1.上海农林职业技术学院，上海 201699； 2.上海交通大学农业与生物学院，上海 200240

Author(s):

Li Baochang; et al

关键词:

黄瓜; NAC转录因子; 盐胁迫; qRT-PCR; 表达水平

Keywords:

-

分类号:

S642.203

DOI:

-

文献标志码:

A

摘要:

黄瓜对盐胁迫非常敏感，土壤中盐含量过高会严重影响其正常生长和发育，从而影响黄瓜的品质和产量。为了筛选黄瓜耐盐相关基因，采用同源克隆技术，从黄瓜叶片的cDNA中克隆了1个NAC转录因子CsNAC032。CsNAC032基因CDS长900 bp，编码299个氨基酸，相对分子质量为34 271.63，等电点为5.67。亚细胞定位预测CsNAC032定位于细胞核。序列比对和进化分析表明，CsNAC032有NAC家族蛋白典型的保守结构域，在N端约150个氨基酸中包含A、B、C、D、E等5个保守的亚结构域，CsNAC032与甜瓜的CmNAC-like （NP_001284423.1）的亲缘关系最近。通过qRT-PCR检测CsNAC032基因在黄瓜不同器官中的表达情况，发现其在主要在根、茎、子叶、果刺等营养器官中表达，暗示其可能主要参与营养器官的发育。在黄瓜幼苗受盐胁迫后3 h，CsNAC032表达水平升高，并于处理后12 h表达水平达到最高，说明CsNAC032可能在黄瓜抵御盐胁迫中发挥着一定功能。

Abstract:

-

参考文献/References:

［1］Golldack D，Lüking I，Yang O. Plant tolerance to drought and salinity： stress regulating transcription factors and their functional significance in the cellular transcriptional network［J］. Plant Cell Reports，2011，30(8)：1383-1391.
［2］Souer E，van Houwelingen A V，Kloos D，et al. The no apical meristem gene of Petunia is required for pattern formation in embryos and flowers and is expressed at meristem and primordia boundaries［J］. Cell，1996，85(2)：159-170.
［3］Ooka H，Satoh K，Doi K，et al. Comprehensive analysis of NAC family genes in Oryza sativa and Arabidopsis thaliana［J］. DNA Research，2003，10(6)：239-247.
［4］Olsen A N，Ernst H A，Leggio L L，et al. NAC transcription factors： structurally distinct，functionally diverse［J］. Trends in Plant Science，2005，10(2)：79-87.
［5］Shao H B，Wang H Y，Tang X L. NAC transcription factors in plant multiple abiotic stress responses： progress and prospects［J］. Frontiers in Plant Science，2015，6：902.
［6］Mahmood K，El-Kereamy A，Kim S H，et al. ANAC032 positively regulates age-dependent and stress-induced senescence in Arabidopsis thaliana［J］. Plant Cell Physiol，2016，57(10)：2029-2046.
［7］Mahmood K，Xu Z H，El-Kereamy A，et al. The Arabidopsis transcription factor ANAC032 represses anthocyanin biosynthesis in response to high sucrose and oxidative and abiotic stresses［J］. Frontiers in Plant Science，2016，7：1548.
［8］Jensen M K，Skriver K. NAC transcription factor gene regulatory and protein-protein interaction networks in plant stress responses and senescence［J］. IUBMB Life，2014，66(3)：156-166.
［9］赖伟，何鹏，徐明远，等. 黄瓜耐盐性与耐盐相关基因的研究进展［J］. 中国蔬菜，2020(6)：16-22.
［10］刘东让，董邵云，苗晗，等. 黄瓜耐盐胁迫遗传育种研究进展［J］. 中国蔬菜，2021(7)：14-23.
［11］曹齐卫，杜连达，杨宗辉，等. 黄瓜耐盐种质资源的筛选与评价［J］. 核农学报，2022，36(5)：865-875.
［12］Zhu H Y，He M W，Jahan M S，et al. CsCDPK6，a CsSAMS1-interacting protein，affects polyamine/ethylene biosynthesis in cucumber and enhances salt tolerance by overexpression in tobacco［J］. International Journal of Molecular Sciences，2021，22(20)：11133.
［13］Liu X W，Wang T，Bartholomew E，et al. Comprehensive analysis of NAC transcription factors and their expression during fruit spine development in cucumber (Cucumis sativus L.)［J］. Horticulture Research，2018，5：31.
［14］Livak K J，Schmittgen T D. Analysis of relative gene expression data using real-time quantitative PCR and the 22^-ΔΔC_T method［J］. Methods，2001，25(4)：402-408.
［15］Wang G R，Yuan Z，Zhang P Y，et al. Genome-wide analysis of NAC transcription factor family in maize under drought stress and rewatering［J］. Physiol Mol Biol Plants，2020，26(4)：705-717.
［16］Diao W P，Snyder J C，Wang S B，et al. Genome-wide analyses of the NAC transcription factor gene family in pepper (Capsicum annuum L.)： chromosome location，phylogeny，structure，expression patterns，cis-elements in the promoter，and interaction network［J］. International Journal of Molecular Sciences，2018，19(4)：1028.
［17］Thirumalaikumar V P，Devkar V，Mehterov N，et al. NAC transcription factor JUNGBRUNNEN1 enhances drought tolerance in tomato［J］. Plant Biotechnology Journal，2018，16(2)：354-366.
［18］Liu X H，Lyu Y S，Yang W P，et al. A membrane-associated NAC transcription factor OsNTL3 is involved in thermotolerance in rice［J］. Plant Biotechnology Journal，2020，18(5)：1317-1329.
［19］Mao H D，Li S M，Chen B，et al. Variation in cis-regulation of a NAC transcription factor contributes to drought tolerance in wheat［J］. Molecular Plant，2022，15(2)：276-292.
［20］Wang Q，Guo C，Li Z Y，et al. Potato NAC transcription factor StNAC053 enhances salt and drought tolerance in transgenic Arabidopsis［J］. International Journal of Molecular Sciences，2021，22(5)：2568.
［21］Ju Y L，Yue X F，Min Z，et al. VvNAC17，a novel stress-responsive grapevine (Vitis vinifera L.) NAC transcription factor，increases sensitivity to abscisic acid and enhances salinity，freezing，and drought tolerance in transgenic Arabidopsis［J］. Plant Physiology and Biochemistry，2020，146：98-111.
［22］Yuan X，Wang H，Cai J T，et al. Rice NAC transcription factor ONAC066 functions as a positive regulator of drought and oxidative stress response［J］. BMC Plant Biol，2019，19(1)：278.

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备注/Memo

备注/Memo:

收稿日期：2022-05-28
基金项目： 上海自然科学基金(编号：20ZR1439600)；上海市“科技创新行动计划”农业领域项目(编号：20392001300)；上海农林职业技术学院中青年领军人才项目(编号：A2-0273-20-01-16)；上海农林职业技术学院校内课题(编号：KY2-0000-20-01)。
作者简介： 李宝昌(1970—)，男，河北大名人，硕士，副教授、高级工程师，主要从事植物栽培、生理与育种研究，E-mail：13321960916@qq.com；共同第一作者：陈岳(1991—)，男，山西大同人，博士，主要从事植物栽培与育种研究，E-mail：yuechen321@aliyun.com。
通信作者： 张涵，硕士，讲师、工程师，主要从事植物栽培、生理与育种研究，E-mail：249848244@qq.com；张微微，博士，教授，主要从事植物栽培与育种研究，E-mail：zhangww@shafc.edu.cn。

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更新日期/Last Update: 2022-09-20