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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:

第50卷

期数:

2022年第18期

页码:

75-81

栏目:

基因组学与功能基因

出版日期:

2022-09-20

文章信息/Info

Title:

Molecular cloning，characterization and expression analysis of three genes in tobacco (Nicotiana tabacum L.) jasmonic acid biosynthesis pathway

作者:

武兆云; 孙聚涛; 张智强; 薛刚; 杨铁钊

河南农业大学烟草学院，河南郑州 450002

Author(s):

Wu Zhaoyun; et al

关键词:

普通烟草; 脂氧合酶; 丙二烯氧化物合酶; 丙二烯氧化物环化酶; 分子克隆; 生物信息学分析; 表达分析

Keywords:

-

分类号:

S572.03

DOI:

-

文献标志码:

A

摘要:

以普通烟草(Nicotiana tabacum L.)为材料，同源克隆烟草NtLOX、NtAOS和NtAOC基因，对其序列进行分析，构建过表达载体和进行低钾胁迫表达分析。蛋白质序列比对和进化树分析结果表明，NtLOX、NtAOS分别属于植物脂氧合酶(IPR001246)、细胞色素P450(IPR002403)家族，其中NtAOC具有异构酶活性(GO：0016853)。根据氨基酸序列预测结果，NtAOS、NtAOC定位于叶绿体，而NtLOX定位于细胞质。为了对基因功能进行研究，构建3个基因的过表达载体。qRT-PCR分析结果表明，在缺钾条件下，与低钾不耐受(敏感)基因型烟草品种相比，耐低钾基因型中NtLOX在转录物水平升高得更早且持续时间更长。因此，NtLOX的表达水平可能与烟草植物对低钾胁迫的抗性密切相关。研究结果为揭示NtLOX、NtAOS和NtAOC在低钾胁迫中的分子机制奠定了研究基础。

Abstract:

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备注/Memo

备注/Memo:

收稿日期：2021-10-13
基金项目：中国烟草总公司特色优质烟叶开发重大专项(编号：Ts-01-2011003)；河南农业大学科技创新基金(编号：KJCX2015A09)；河南省高校重点科研项目(编号：17B210004)。
作者简介：武兆云(1979－)，男，安徽马鞍山人，博士，讲师，主要从事烟草育种研究。E-mail：wuzhaoyun@henau.edu.cn。
通信作者：杨铁钊，硕士，教授，主要从事烟草育种研究。E-mail：yangtiezhao@outlook.com。

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更新日期/Last Update: 2022-09-20