|本期目录/Table of Contents|

[1]冯献君,吴英华,史艳,等.菜心酸性蔗糖转化酶基因家族的鉴定及表达分析[J].江苏农业科学,2022,50(19):44-50.
 Feng Xianjun,et al.Identification and expression analysis of acid sucrose invertase gene family in Chinese flowering cabbage[J].Jiangsu Agricultural Sciences,2022,50(19):44-50.
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菜心酸性蔗糖转化酶基因家族的鉴定及表达分析(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第50卷
期数:
2022年第19期
页码:
44-50
栏目:
生物技术
出版日期:
2022-10-05

文章信息/Info

Title:
Identification and expression analysis of acid sucrose invertase gene family in Chinese flowering cabbage
作者:
冯献君吴英华史艳侯雷平李梅兰
山西农业大学园艺学院,山西太谷 030801
Author(s):
Feng Xianjunet al
关键词:
菜心酸性蔗糖转化酶基因家族生物信息学基因表达
Keywords:
-
分类号:
S634.501
DOI:
-
文献标志码:
A
摘要:
酸性蔗糖转化酶包括细胞壁蔗糖转化酶和液泡蔗糖转化酶,该家族的基因是植物体内蔗糖代谢的关键基因。为了更好地了解该基因家族在菜心蔗糖代谢调控过程中的作用机制,对该基因家族成员的理化性质、染色体定位、系统进化树、蛋白质二级结构预测及亚细胞定位、基因结构和保守基序进行生物信息学分析,同时测定该基因家族成员在菜心不同器官中的表达量。结果共鉴定出13个菜心酸性蔗糖转化酶基因家族成员,这些基因分为2类,其中有9个为细胞壁蔗糖转化酶基因(CWINV),4个为液泡蔗糖转化酶基因(VINV),不均匀地分布在7条染色体上。菜心酸性蔗糖转化酶基因家族的蛋白质氨基酸数量大部分集中在500~650个范围内,分子量变化与氨基酸数量变化趋势一致。其蛋白质的二级结构无规则卷曲所占比例最高。该家族蛋白质大部分被定位在细胞质和周质中。基因结构分析结果显示,13个基因中有8个基因均含有10个保守基序。部分酸性蔗糖转化酶存在外显子丢失的情况,大部分基因均含有6个或7个外显子。13个酸性蔗糖转化酶基因在菜心薹茎和花蕾中高表达的成员较多。这为进一步研究菜心酸性蔗糖转化酶基因家族成员在蔗糖代谢调控中的作用提供了数据基础。
Abstract:
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备注/Memo

备注/Memo:
收稿日期:2021-11-14
基金项目:山西省重点研发计划重点项目(编号:201703D211001-04-01);山西省重点研发计划(编号:201803D221005-8);山西农业大学曲沃果蔬研究院项目(编号:2021QWGS-3)。
作者简介:冯献君(1996—),女,山西吕梁人,硕士研究生,主要从事蔬菜育种及生物技术应用研究。E-mail:643046085@qq.com。
通信作者:侯雷平,硕士,教授,主要从事设施园艺、蔬菜栽培以及园艺植物生物技术方面的教学与研究,E-mail:sxndhlp@126.com;李梅兰,博士,教授,主要从事蔬菜育种及生物技术应用研究,E-mail:15935485975@163.com。
更新日期/Last Update: 2022-10-05