[1]叶欣悦,闫见敏,杨雪莲,等.SlCOMT1基因克隆及在番茄组织器官中的表达和褪黑素生物合成变化[J].江苏农业科学,2022,50(23):49-54.
Ye Xinyue,et al.Cloning of SlCOMT1 gene and its expression in tomato tissues and melatonin biosynthesis[J].Jiangsu Agricultural Sciences,2022,50(23):49-54.
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SlCOMT1基因克隆及在番茄组织器官中的表达和褪黑素生物合成变化(PDF)
Ye Xinyue,et al.Cloning of SlCOMT1 gene and its expression in tomato tissues and melatonin biosynthesis[J].Jiangsu Agricultural Sciences,2022,50(23):49-54.
《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]
- 卷:
- 第50卷
- 期数:
- 2022年第23期
- 页码:
- 49-54
- 栏目:
- 生物技术
- 出版日期:
- 2022-12-05
文章信息/Info
- Title:
- Cloning of SlCOMT1 gene and its expression in tomato tissues and melatonin biosynthesis
- 关键词:
- 番茄; 咖啡酸-O-甲基转移酶; 基因克隆; 褪黑素; 表达模式
- Keywords:
- -
- 分类号:
- S641.201
- DOI:
- -
- 文献标志码:
- A
- 摘要:
- 为探讨咖啡酸-O-甲基转移酶关键基因(SlCOMT1)在番茄不同生育期和组织器官中的表达特异性和褪黑素含量变化，采用RT-PCR技术从矮生型番茄Micro-Tom叶片中克隆了SlCOMT1基因，其全长1 074 bp，编码357个氨基酸。生物信息学分析结果表明，SlCOMT1蛋白具有SAM(S-腺苷甲硫氨酸)依赖型甲基转移酶超家族典型的保守结构域，不含跨膜结构，是一种稳定的高脂溶性蛋白，含有31个磷酸化位点，二级结构以α螺旋为主。系统进化树和保守基序分析结果表明，该蛋白序列与茄科的辣椒和马铃薯具有较高的亲缘关系且含有相同的保守基序。荧光定量PCR和内源褪黑素含量分析结果表明，SlCOMT1和褪黑素在番茄不同组织器官中均有表达和积累，分别在开花后5 d、花苞中表达量最高，在其他组织中SlCOMT1的表达和褪黑素含量之间存在一定的相关性。本研究结果可为深入研究SlCOMT1基因的生物学功能提供理论依据。
- Abstract:
- -
参考文献/References:
［1］Tan D X，Manchester L C，Reiter R J，et al. Significance of melatonin in antioxidative defense system：reactions and products［J］. Biological Signals and Receptors，2000，9(3/4)：137-159.
［2］Rosen J，Than N N，Koch D，et al. Interactions of melatonin and its metabolites with the ABTS cation radical：extension of the radical scavenger cascade and formation of a novel class of oxidation products，C2-substituted 3-indolinones［J］. Journal of Pineal Research，2006，41(4)：374-381.
［3］Tan D X，Manchester L C，Terron M P，et al. One molecule，many derivatives：a never-ending interaction of melatonin with reactive oxygen and nitrogen species？［J］. Journal of Pineal Research，2007，42(1)：28-42.
［4］Fan J B，Xie Y，Zhang Z C，et al. Melatonin：a multifunctional factor in plants［J］. International Journal of Molecular Sciences，2018，19(5)：1528.
［5］Back K.Melatonin metabolism，signaling and possible roles in plants［J］. The Plant Journal，2021，105(2)：376-391.
［6］Wang Y P，Reiter R J，Chan Z L.Phytomelatonin：a universal abiotic stress regulator［J］. Journal of Experimental Botany，2017，69(5)：963-974.
［7］Wolf K，Kolárˇ J A N，Witters E，et al. Daily profile of melatonin levels in Chenopodium rubrum L. depends on photoperiod［J］. Journal of Plant Physiology，2001，158(11)：1491-1493.
［8］Murch S J，Campbell S S B，Saxena P K.The role of serotonin and melatonin in plant morphogenesis：regulation of auxin-induced root organogenesis in in vitro-cultured explants of st［J］. In Vitro Cellular & Developmental Biology-Plant，2001，37(6)：786-793.
［9］Xiao S，Liu L T，Wang H，et al. Exogenous melatonin accelerates seed germination in cotton (Gossypium hirsutum L.)［J］. PLoS One，2019，14(6)：e0216575.
［10］Byeon Y，Lee H Y，Lee K，et al. Caffeic acid O-methyltransferase is involved in the synthesis of melatonin by methylating N-acetylserotonin in Arabidopsis［J］. Journal of Pineal Research，2014，57(2)：219-227.
［11］Yan Y Y，Sun S S，Zhao N，et al. COMT1 overexpression resulting in increased melatonin biosynthesis contributes to the alleviation of carbendazim phytotoxicity and residues in tomato plants［J］. Environmental Pollution，2019，252：51-61.
［12］Ahammed G J，Xu W，Liu A R，et al. COMT1 silencing aggravates heat stress-induced reduction in photosynthesis by decreasing chlorophyll content，photosystem Ⅱ activity，and electron transport efficiency in tomato［J］. Frontiers in Plant Science，2018，9：998.
［13］Aoki K，Yano K，Suzuki A，et al. Large-scale analysis of full-length cDNAs from the tomato (Solanum lycopersicum) cultivar Micro-Tom，a reference system for the Solanaceae genomics［J］. BMC Genomics，2010，11：210.
［14］Liu D D，Sun X S，Liu L，et al. Overexpression of the melatonin synthesis-related gene SlCOMT1 improves the resistance of tomato to salt stress［J］. Molecules，2019，24(8)：1514.
［15］Chang J J，Guo Y L，Yan J Y，et al. The role of watermelon caffeic acid O-methyltransferase (ClCOMT1) in melatonin biosynthesis and abiotic stress tolerance［J］. Horticulture Research，2021，8：210.
［16］周露，化文平，杨滢，等. 丹参法呢基焦磷酸合酶基因(SmFPPS1)的表达模式［J］. 陕西师范大学学报(自然科学版)，2013，41(2)：70-75.
［17］Byeon Y，Choi G H，Lee H Y，et al. Melatonin biosynthesis requires N-acetylserotonin methyltransferase activity of caffeic acid O-methyltransferase in rice［J］. Journal of Experimental Botany，2015，66(21)：6917-6925.
［18］Yang W J，Du Y T，Zhou Y B，et al. Overexpression of TaCOMT improves melatonin production and enhances drought tolerance in transgenic Arabidopsis［J］. International Journal of Molecular Sciences，2019，20(3)：652.
［19］千春录，朱芹，高姗，等. 外源褪黑素处理对采后水蜜桃冷藏品质及冷害发生的影响［J］. 江苏农业学报，2020，36(3)：702-708.
［20］Okazaki M，Ezura H.Profiling of melatonin in the model tomato (Solanum lycopersicum L.) cultivar Micro-Tom［J］. Journal of Pineal Research，2009，46(3)：338-343.
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备注/Memo
- 备注/Memo:
-
收稿日期：2022-02-14
基金项目：国家自然科学基金(编号：31760594)；贵州省“千”层次人才培养项目(编号：贵大“千”层次［2018］02号)；贵州省自然科学基金(编号：黔科合基础［2018］1041)。
作者简介：叶欣悦(1993—)，男，贵州贵阳人，硕士研究生，主要从事番茄逆境调控生理及分子机制研究。E-mail：yexinyue1993@163.com。
通信作者：须文，博士，教授，主要从事蔬菜生长发育调控研究。E-mail：wxu1@gzu.edu.cn。
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