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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:

第50卷

期数:

2022年第24期

页码:

131-135

栏目:

畜牧兽医与水产蚕桑

出版日期:

2022-12-20

文章信息/Info

Title:

Analysis of MYL2 gene polymorphism and its differential expression in muscular tissue of pigs

作者:

叶幼荣¹; 2; 3; 张健¹; 2; 3; 赵舜旺¹; 2; 徐士军¹; 2; 刘振东¹; 2; 强巴央宗¹; 2; 3; 商鹏¹; 2; 3

1.西藏农牧学院，西藏林芝 860000； 2.西藏特色农牧资源研发省部共建协同创新中心，西藏林芝 860000；3.西藏自治区林芝藏猪农业科技园区，西藏林芝 860000

Author(s):

Ye Yourong; et al

关键词:

MYL2基因; SNP; 藏猪; 转录因子; 相对表达量

Keywords:

-

分类号:

S828.2

DOI:

-

文献标志码:

A

摘要:

肌球蛋白轻链-2(myosin light chain 2，MYL2)是哺乳动物横纹肌中的一种主要肌小节蛋白，对动物正常生长发育起重要调控作用，为探究猪MYL2基因的多态性及其在横纹肌中mRNA表达规律，以藏猪(TP)和大约克猪(YY)作为试验动物，采用Sanger测序法对MYL2基因5′非翻译区(5′UTR)进行单核苷酸多态性(SNP)的筛选与基因分型，并预测分析MYL2基因SNPs位点突变前后转录因子功能特性；同时，采用RT-qPCR技术检测MYL2基因在30、90、180日龄时TP和YY的心肌和背最长肌组织中的mRNA相对表达量。结果显示，MYL2基因启动子区域存在2个SNPs且2个品种间基因频率差异极显著(P<0.01)，产生4个新增转录因子结合位点，这些位点调控横纹肌的收缩、肌纤维类型的维持、肌肉细胞的增殖分化等过程；MYL2基因的mRNA水平随日龄增长而升高，且3个日龄TP心肌和背最长肌组织中的表达量均极显著高于YY(P<0.01)。综上表明，猪MYL2基因SNPs位点与心肌和背最长肌mRNA的差异表达相关，研究结果可为研究藏猪生长发育的分子调控机制提供一定的理论依据。

Abstract:

-

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备注/Memo

备注/Memo:

收稿日期：2022-02-09
基金项目：西藏自治区重大科技专项项目(编号：XZ202101ZD0005N)；林芝市巴宜区重点研发计划(编号：2021-GX-SY-01)；四川省区域创新合作项目(编号：2020YFQ0029)。
作者简介：叶幼荣(1989—)，女，河南信阳人，硕士，讲师，主要从事动物遗传育种与繁殖学研究。E-mail：yeyourong@xza.edu.cn。
通信作者 ：商鹏，博士，副教授，主要从事动物功能基因组研究。E-mail：nemoshpmh@126.com。

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更新日期/Last Update: 2022-12-20