|本期目录/Table of Contents|

[1]王健,张珍珍,梅秀珍,等.黄酮类化合物的抗菌作用及机制研究进展[J].江苏农业科学,2023,51(1):1-8.
　Wang Jian??et al.Research progress on antibacterial effects and mechanism of flavonoids[J].Jiangsu Agricultural Sciences,2023,51(1):1-8.
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黄酮类化合物的抗菌作用及机制研究进展(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第51卷
期数:
2023年第1期
页码:
1-8
栏目:
专论与综述
出版日期:
2023-01-05

文章信息/Info

Title:
Research progress on antibacterial effects and mechanism of flavonoids
作者:
王健1; 2; 张珍珍2; 3; 梅秀珍2; 3; 徐业芬1; 冯志新1; 2; 3
1.西藏农牧学院动物科学学院，西藏林芝 860000； 2.江苏省农业科学院兽医研究所，江苏南京 210014；3.南京农业大学动物医学院，江苏南京 210095
Author(s):
Wang Jian??et al
关键词:
黄酮类化合物; 抗菌机制; 抗生素
Keywords:
-
分类号:
R914
DOI:
-
文献标志码:
A
摘要:
广泛使用抗生素治疗细菌性疾病，会导致细菌耐药性逐渐提升，因此，亟需寻找替代抗生素的新型药物。大量研究表明，中药化学成分具有较好的抗菌活性，其中黄酮类化合物作为一种广泛存在于蔬菜、水果、谷物和茶叶中的天然多酚物质，黄酮类化合物在抗菌领域作为抗菌药物或抗菌联用药物受到越来越多的关注。本文将系统总结黄酮类化合物的抗菌机制。黄酮类化合物的抗菌机制可以分为2个方面，一方面直接抑制细菌生长，包括改变细胞膜通透性引起细胞质损伤、抑制核酸合成、抑制能量代谢、细胞膜损伤、细胞壁损伤等；另一方面是通过调控宿主细胞间接发挥抑菌作用，药物作用能调控相关信号通路，抑制促炎细胞因子的释放，调节免疫细胞分化、活化从而抑制细菌。黄酮类化合物抗菌作用的研究为今后探索替代抗生素药物或者减少抗生素使用剂量方面提供了新的思路。
Abstract:
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参考文献/References:

［1］Sultan I，Rahman S，Jan A T，et al. Antibiotics，resistome and resistance mechanisms：a bacterial perspective［J］. Frontiers in Microbiology，2018，9：2066.
［2］Sousa C F，Coimbra J T S，Gomes I，et al. The binding of free and copper-complexed fluoroquinolones to OmpF porins：an experimental and molecular docking study［J］. RSC Advances，2017，7(17)：10009-10019.
［3］Golkar T，Bassenden A V，Maiti K，et al. Structural basis for plazomicin antibiotic action and resistance［J］. Communications Biology，2021，4(1)：729.
［4］Maleki S J，Crespo J F，Cabanillas B. Anti-inflammatory effects of flavonoids［J］. Food Chemistry，2019，299：125124.
［5］Navrátilová A，Neuta O，Vancˇatová I，et al. C-geranylated flavonoids from Paulownia tomentosa fruits with antimicrobial potential and synergistic activity with antibiotics［J］. Pharmaceutical Biology，2016，54(8)：1398-1407.
［6］Shamsudin N F，Ahmed Q U，Mahmood S，et al. Antibacterial effects of flavonoids and their structure-activity relationship study：a comparative interpretation［J］. Molecules，2022，27(4)：1149.
［7］Nag D，Dastidar D G，Chakrabarti G. Natural flavonoid morin showed anti-bacterial activity against Vibrio cholera after binding with cell division protein FtsA near ATP binding site［J］. Biochimica et Biophysica Acta，2021，1865(8)：129931.
［8］Jiang F，Guan H N，Liu D Y，et al. Flavonoids from sea buckthorn inhibit the lipopolysaccharide-induced inflammatory response in RAW264.7 macrophages through the MAPK and NF-κB pathways［J］. Food & Function，2017，8(3)：1313-1322.
［9］Santos-Buelga C，Feliciano A S. Flavonoids：from structure to health issues［J］. Molecules，2017，22(3)：477.
［10］Shen N，Wang T F，Gan Q，et al. Plant flavonoids：classification，distribution，biosynthesis，and antioxidant activity［J］. Food Chemistry，2022，383：132531.
［11］Veloz J J，Alvear M，Salazar L A. Antimicrobial and antibiofilm activity against Streptococcus mutans of individual and mixtures of the main polyphenolic compounds found in Chilean Propolis［J］. BioMed Research International，2019，2019：7602343.
［12］闻永举，申秀丽. 柚皮苷半合成芹菜素的研究［J］. 江苏农业科学，2012，40(5)：252-253.
［13］Guo Y R，Liu Y，Zhang Z H，et al. The antibacterial activity and mechanism of action of luteolin against Trueperella pyogenes［J］. Infection and Drug Resistance，2020，13：1697-1711.
［14］Singla R K，Dubey A K，Garg A，et al. Natural polyphenols：chemical classification，definition of classes，subcategories，and structures［J］. Journal of AOAC International，2019，102(5)：1397-1400.
［15］Moodi Z，Bagherzade G，Peters J. Quercetin as a precursor for the synthesis of novel nanoscale Cu(Ⅱ) complex as a catalyst for alcohol oxidation with high antibacterial activity［J］. Bioinorganic Chemistry and Applications，2021，2021：8818452.
［16］徐海瑛，郝红英，陈青阁，等. 鱼腥草活性成分槲皮素及其金属配合物的抗菌活性［J］. 江苏农业科学，2018，46(21)：218-220.
［17］Wang T T，Zhang P，Lv H F，et al. A natural dietary flavone myricetin as an α-hemolysin inhibitor for controlling Staphylococcus aureus infection［J］. Frontiers in Cellular and Infection Microbiology，2020，10：330.
［18］Zhang Y N，Yin H M，Zhang Y，et al. Cocrystals of kaempferol，quercetin and myricetin with 4，4′-bipyridine：crystal structures，analyses of intermolecular interactions and antibacterial properties［J］. Journal of Molecular Structure，2017，1130：199-207.
［19］Barreca D，Gattuso G，Bellocco E，et al. Flavanones：Citrus phytochemical with health-promoting properties［J］. BioFactors，2017，43(4)：495-506.
［20］Yue J X，Yang H Y，Liu S Y，et al. Influence of naringenin on the biofilm formation of Streptococcus mutans［J］. Journal of Dentistry，2018，76：24-31.
［21］岳嘉曦，刘思颖，杨宏业，等. 柚皮素对变形链球菌生物膜形成的抑制作用［C］//中华口腔医学会口腔修复学专业委员会第十次全国口腔修复学术大会论文集.杭州，2016：231-232.
［22］Zhang L，Han Z S，Granato D. Polyphenols in foods：classification，methods of identification，and nutritional aspects in human health［J］. Advances in Food and Nutrition Research，2021，98：1-33.
［23］黄仁术，易凡. 金荞麦(-)表儿茶素类活性物质体外抑菌试验［J］. 江苏农业科学，2015，43(1)：308-310.
［24］Zhang G F，Tan Y，Yu T S，et al. Synergistic antibacterial effects of reuterin and catechin against Streptococcus mutans［J］. LWT-Food Science and Technology，2021，139：110527.
［25］Krˇíová L，Dadáková K，Kaparovská J，et al. Isoflavones［J］. Molecules，2019，24(6)：1076.
［26］顾和平，陈新，陈华涛，等. 大豆异黄酮药理效应研究进展［J］. 江苏农业科学，2012，40(9)：19-22.
［27］Vázquez L，Flórez A B，Guadamuro L，et al. Effect of soy isoflavones on growth of representative bacterial species from the human gut［J］. Nutrients，2017，9(7)：727.
［28］Doerge D R，Sheehan D M. Goitrogenic and estrogenic activity of soy isoflavones［J］. Environmental Health Perspectives，2002，110(S3)：349-353.
［29］Genskowsky E，Puente L A，Pérez-lvarez J A，et al. Determination of polyphenolic profile，antioxidant activity and antibacterial properties of maqui［Aristotelia chilensis (Molina) Stuntz］a Chilean blackberry［J］. Journal of the Science of Food and Agriculture，2016，96(12)：4235-4242.
［30］Li L L，Zhou P，Wang Y D，et al. Antimicrobial activity of cyanidin-3-O-glucoside-lauric acid ester against Staphylococcus aureus and Escherichia coli［J］. Food Chemistry，2022，383：132410.
［31］Tammela P，Laitinen L，Galkin A，et al. Permeability characteristics and membrane affinity of flavonoids and alkyl gallates in Caco-2 cells and in phospholipid vesicles［J］. Archives of Biochemistry and Biophysics，2004，425(2)：193-199.
［32］吴婷. 基于膜交互作用的黄酮类化合物抑制大肠杆菌的机理及定量构效关系研究［D］. 武汉：华中农业大学，2014.
［33］Wen Q H，Wang R，Zhao S Q，et al. Inhibition of biofilm formation of foodborne Staphylococcus aureus by the Citrus flavonoid naringenin［J］. Foods，2021，10(11)：2614.
［34］Eumkeb G，Chukrathok S. Synergistic activity and mechanism of action of ceftazidime and apigenin combination against ceftazidime-resistant Enterobacter cloacae［J］. Phytomedicine，2013，20(3/4)：262-269.
［35］Chinnam N，Dadi P K，Sabri S A，et al. Dietary bioflavonoids inhibit Escherichia coli ATP synthase in a differential manner［J］. International Journal of Biological Macromolecules，2010，46(5)：478-486.
［36］Denny W A. Inhibitors of F1F0-ATP synthase enzymes for the treatment of tuberculosis and cancer［J］. Future Medicinal Chemistry，2021，13(10)：911-926.
［37］Gledhill J R，Montgomery M G，Leslie A G，et al. Mechanism of inhibition of bovine F1-ATPase by resveratrol and related polyphenols［J］. Proceedings of the National Academy of Sciences of the United States of America，2007，104(34)：13632-13637.
［38］Plaper A，Golob M，Hafner I，et al. Characterization of quercetin binding site on DNA gyrase［J］. Biochemical and Biophysical Research Communications，2003，306(2)：530-536.
［39］Weidner-Wells M A，Altom J，Fernandez J，et al. DNA gyrase inhibitory activity of ellagic acid derivatives［J］. Bioorganic & Medicinal Chemistry Letters，1998，8(1)：97-100.
［40］He J，Qiao W L，An Q，et al. Dihydrofolate reductase inhibitors for use as antimicrobial agents［J］. European Journal of Medicinal Chemistry，2020，195：112268.
［41］Khan N，Niazi Z R，Rehman F U，et al. Hyaluronidases：a therapeutic enzyme［J］. Protein and Peptide Letters，2018，25(7)：663-676.
［42］Disbanchong P，Punmanee W，Srithanasuwan A，et al. Immunomodulatory effects of herbal compounds quercetin and curcumin on cellular and molecular functions of bovine-milk-isolated neutrophils toward Streptococcus agalactiae infection［J］. Animals，2021，11(11)：3286.
［43］Zuo A R，Dong H H，Yu Y Y，et al. The antityrosinase and antioxidant activities of flavonoids dominated by the number and location of phenolic hydroxyl groups［J］. Chinese Medicine，2018，13(1)：51.
［44］Fathima A，Rao J R. Selective toxicity of Catechin—a natural flavonoid towards bacteria［J］. Applied Microbiology and Biotechnology，2016，100(14)：6395-6402.
［45］Zhang S P，Hu B，Xu J F，et al. Baicalin suppress growth and virulence-related factors of methicillin-resistant Staphylococcus aureus in vitro and in vivo［J］. Microbial Pathogenesis，2020，139：103899.
［46］尹良军，周振旗，绍元，等. 盐酸小檗碱、人参皂苷Rb1、黄芩苷及绿原酸对大肠埃希菌和金黄色葡萄球菌的抑菌作用研究［J］. 医学综述，2016，22(24)：4969-4972.
［47］Taylor P W. Interactions of tea-derived catechin gallates with bacterial pathogens［J］. Molecules，2020，25(8)：1986.
［48］Chang E H，Giaquinto P，Huang J，et al. Epigallocatechin gallate inhibits leukotoxin release by Aggregatibacter actinomycetemcomitans by promoting association with the bacterial membrane［J］. Molecular Oral Microbiology，2020，35(1)：29-39.
［49］Górniak I，Bartoszewski R，Króliczewski J. Comprehensive review of antimicrobial activities of plant flavonoids［J］. Phytochemistry Reviews，2019，18(1)：241-272.
［50］Li R，Lu J Y，Duan H B，et al. Biofilm inhibition and mode of action of epigallocatechin gallate against Vibrio mimicus［J］. Food Control，2020，113：107148.
［51］Brown A K，Papaemmanouil A，Bhowruth V，et al. Flavonoid inhibitors as novel antimycobacterial agents targeting Rv0636，a putative dehydratase enzyme involved in Mycobacterium tuberculosis fatty acid synthase Ⅱ［J］. Microbiology，2007，153(10)：3314-3322.
［52］Yi R K，Wang F B，Tan F，et al. Intervention effects of lotus leaf flavonoids on gastric mucosal lesions in mice infected with Helicobacter pylori［J］. RSC Advances，2020，10(40)：23510-23521.
［53］Skiba M A，Szendzielorz K，Mazur B，et al. The inhibitory effect of flavonoids on interleukin-8 release by human gastric adenocarcinoma (AGS) cells infected with cag PAI (+) Helicobacter pylori［J］. Central-European Journal of Immunology，2016，41(3)：229-235.
［54］González A，Casado J，Lanas .Fighting the antibiotic crisis：flavonoids as promising antibacterial drugs against Helicobacter pylori infection［J］. Frontiers in Cellular and Infection Microbiology，2021，11：709749.
［55］Wang Y C，Huang K M. In vitro anti-inflammatory effect of apigenin in the Helicobacter pylori-infected gastric adenocarcinoma cells［J］. Food and Chemical Toxicology，2013，53：376-383.
［56］Markandey M，Bajaj A，Ilott N E，et al. Gut microbiota：sculptors of the intestinal stem cell niche in health and inflammatory bowel disease［J］. Gut Microbes，2021，13(1)：1990827.
［57］Li B L，Du P L，Du Y，et al. Luteolin alleviates inflammation and modulates gut microbiota in ulcerative colitis rats［J］. Life Sciences，2021，269：119008.
［58］Polewski M A，Esquivel-Alvarado D，Wedde N S，et al. Isolation and characterization of blueberry polyphenolic components and their effects on gut barrier dysfunction［J］. Journal of Agricultural and Food Chemistry，2020，68(10)：2940-2947.
［59］Chen C R，Wang J J，Chen J F，et al. Morusin alleviates Mycoplasma pneumonia via the inhibition of Wnt/β-catenin and NF-κB signaling［J］. Bioscience Reports，2019，39(6)：BSR20190190.
［60］Zou M，Yang L，Niu L，et al. Baicalin ameliorates Mycoplasma gallisepticum-induced lung inflammation in chicken by inhibiting TLR6-mediated NF-κB signalling［J］. British Poultry Science，2021，62(2)：199-210.
［61］王博，王晓溪，徐慧星，等. 黄芩苷对肺炎支原体感染肺上皮细胞增殖的影响［J］. 中国中医药科技，2021，28(4)：557-560.
［62］Grienke U，Richter M，Walther E，et al. Discovery of prenylated flavonoids with dual activity against influenza virus and Streptococcus pneumoniae［J］. Scientific Reports，2016，6：27156.
［63］Zampini I C，Villena J，Salva S，et al. Potentiality of standardized extract and isolated flavonoids from Zuccagnia punctata for the treatment of respiratory infections by Streptococcus pneumoniae：in vitro and in vivo studies［J］. Journal of Ethnopharmacology，2012，140(2)：287-292.
［64］张国荣，刘楠，刘莉. 肺炎克雷伯菌肺炎对小鼠NLRP3炎症通路的影响［J］. 世界临床药物，2019，40(2)：95-100.
［65］Sun Z J，Li Q，Hou R R，et al. Kaempferol-3-O-glucorhamnoside inhibits inflammatory responses via MAPK and NF-κB pathways in vitro and in vivo［J］. Toxicology and Applied Pharmacology，2019，364：22-28.
［66］王月华，王变变，尹旭升，等. 中国蜂胶水提物抗肿瘤活性功效成分研究［J］. 江苏农业科学，2018，46(12)：153-156.
［67］Cisneros J A，Robertson M J，Mercado B Q，et al. Systematic study of effects of structural modifications on the aqueous solubility of drug-like molecules［J］. ACS Medicinal Chemistry Letters，2017，8(1)：124-127.

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备注/Memo

备注/Memo:
收稿日期：2022-03-10
项目基金：江苏省农业科技自主创新资金［编号：CX(20)1006］。
作者简介：王健(1996—)，男，江苏海安人，硕士研究生，主要从事动物支原体研究。E-mail：1106868641@qq.com。
通信作者：冯志新，博士，研究员，主要从事动物黏膜免疫疫苗与黏膜诊断技术研究。E-mail：fzxjaas@163.com。
更新日期/Last Update: 2023-01-05