|本期目录/Table of Contents|

[1]韩志松,白俊艳,曾凡林,等.小尾寒羊GHR、GHRH、GHRHR基因多态性与肉质性状的关联分析[J].江苏农业科学,2023,51(8):145-149.
 Han Zhisong,et al.Association analysis of polymorphism of GHR,GHRH and GHRHR genes and meat quality traits in small-tailed Han sheep[J].Jiangsu Agricultural Sciences,2023,51(8):145-149.
点击复制

小尾寒羊GHR、GHRH、GHRHR基因多态性与肉质性状的关联分析(PDF)
分享到:

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第51卷
期数:
2023年第8期
页码:
145-149
栏目:
畜牧兽医与水产蚕桑
出版日期:
2023-04-20

文章信息/Info

Title:
Association analysis of polymorphism of GHR,GHRH and GHRHR genes and meat quality traits in small-tailed Han sheep
作者:
韩志松1白俊艳2曾凡林2李静云2何豫涵2陈梦柯2卢小宁2
1.辽宁省沙地治理与利用研究所,辽宁阜新 123000; 2.河南科技大学动物科技学院/洛阳市动物遗传育种重点实验室,河南洛阳 471023
Author(s):
Han Zhisonget al
关键词:
小尾寒羊GHR基因GHRHR基因肉质性状关联分析
Keywords:
-
分类号:
S826.2
DOI:
-
文献标志码:
A
摘要:
主要分析GHR、GHRH、GHRHR基因的多态性,以及其与小尾寒羊肉质性状的关联,以期为优质绵羊新品种培育提供一定的参考依据。采用PCR技术,检测小尾寒羊群体中GHR、GHRH和GHRHR基因的多态性,利用SPSS软件分析以上3种基因的不同基因型与肉质性状的关联性。结果表明,GHR与GHRHR共有3种基因型,分别为II、ID、DD,而GHRH基因仅检测到2种基因型,分别为II、DD。GHR基因中II基因型频率最高,为0.625,ID的基因型频率为0.313,DD的基因型频率最低,为0.063;多态信息含量(PIC)为0.283,处于中度多态性(0.25≤PIC<0.50);小尾寒羊GHR基因的DD基因型肉质的色泽显著低于ID、II基因型(P<0.05)。GHRHR基因中II基因型频率最高,为0.687 5,DD基因型频率最低,为0.062 5,ID基因型频率为0.2500;多态信息含量为0.258,处于中度多态性(0.25≤PIC<050);GHRHR基因的DD基因型的剪切力显著高于ID、II基因型(P<0.05)。GHRH基因中DD基因型频率最低,为0.437 5,II基因型频率最高,为0.562 5;多态信息含量(PIC)为0371,处于中度多态性(0.25≤PIC<0.50);GHRH不同基因型对于小尾寒羊的所有肉质性状影响不显著(P>005)。综上所述,GHR基因、GHRHR基因可以作为小尾寒羊提升肉质品质的候选基因之一。
Abstract:
-

参考文献/References:

[1]Tsushima T. Technics of growth hormone receptor assay[J]. Nihon Rinsho,1976,34(3):461-465.
[2]Gencheva D G,Velikov K P,Veleva P M. Association analysis of nucleotide polymorphisms in growth hormone (GH) and its receptor (GHR) with body weight in Californian rabbits[J]. World Rabbit Science,2022,30(1):95-102.
[3]Cobanoglu O,Kul E,Gurcan E K,et al. Determination of the association of GHR/AluI gene polymorphisms with milk yield traits in Holstein and Jersey cattle raised in Turkey[J]. Archives Animal Breeding,2021,64(2):417-424.
[4]Komisarek J,Michalak A,Walendowska A. The effects of polymorphisms in DGAT1,GH and GHR genes on reproduction and production traits in Jersey cows[J]. Animal Science Papers and Reports,2011,29(1):29-36.
[5]Fedota O M,Lysenko N G,Ruban S Y,et al. The effects of polymorphisms in growth hormone and growth hormone receptor genes on production and reproduction traits in Aberdeen-Angus cattle (Bos taurus L.,1758)[J]. Cytology and Genetics,2017,51(5):352-360.
[6]Erdogˇan M,TekerlI· M,elI·kelogˇlu K,et al. Associations of SNPs in GHR gene with growth and milk yield of Anatolian buffaloes[J]. Turkish Journal of Veterinary and Animal Sciences,2021,45(6):1080-1086.
[7]马冬梅,韩林强,白俊杰. 大口黑鲈GHRH基因启动子区域序列分析及其活性检测[J]. 海洋渔业,2016,38(4):383-390.
[8]王金玲,王永,刘鲁蜀,等. 草地藏系绵羊生长激素释放激素基因部分cDNA的克隆及生物信息学分析[J]. 江苏农业学报,2015,31(3):595-599.
[9]Leone S,Shohreh R,Manippa F,et al. Behavioural phenotyping of male growth hormone-releasing hormone (GHRH) knockout mice[J]. Growth Hormone & IGF Research,2014,24(5):192-197.
[10]Zhang B,Zhao G F,Lan X Y,et al. Polymorphism in GHRH gene and its association with growth traits in Chinese native cattle[J]. Research in Veterinary Science,2012,92(2):243-246.
[11]Ma D M,Han L Q,Bai J J,et al. A 66-bp deletion in growth hormone releasing hormone gene 5′-flanking region with largemouth bass recessive embryonic lethal[J]. Animal Genetics,2014,45(3):421-426.
[12]Thounaojam M C,Powell F L,Patel S,et al. Protective effects of agonists of growth hormone-releasing hormone (GHRH) in early experimental diabetic retinopathy[J]. Proceedings of the National Academy of Sciences of the United States of America,2017,114(50):13248-13253.
[13]Tam J K,Chow B K,Lee L T.Structural and functional divergence of growth hormone-releasing hormone receptors in early sarcopterygians:lungfish and Xenopus[J]. PLoS One,2013,8(1):e53482.
[14]Cohen E,Belkacem S,Fedala S,et al. Contribution of functionally assessed GHRHR mutations to idiopathic isolated growth hormone deficiency in patients without GH1 mutations[J]. Human Mutation,2019,40(11):2033-2043.
[15]Ariyasu D,Kubo E,Higa D,et al. Decreased activity of the GHRHR and GH promoters causes dominantly inherited GH deficiency in humanized GH1 mouse models[J]. Endocrinology,2019,160(11):2673-2691.
[16]杨洁,赫佳,王丹碧,等. InDel标记的研究和应用进展[J]. 生物多样性,2016,24(2):237-243.
[17]张沅. 家畜育种学[M]. 2版.北京:中国农业出版社,2018.
[18]Wu M L,Zhao H D,Tang X Q,et al. Novel InDels of GHR,GHRH,GHRHR and their association with growth traits in seven Chinese sheep breeds[J]. Animals,2020,10(10):1883.
[19]Sharma A,Dutt G,Sivalingam J,et al. Novel SNPs in IGF1,GHR and IGFBP-3 genes reveal significant association with growth traits in Indian goat breeds[J]. Small Ruminant Research,2013,115(1/2/3):7-14.
[20]施雪奎. 肉牛CAPN1、CAST、GHR、SCD1基因多态性及与胴体、肉质性状的相关性研究[D]. 扬州:扬州大学,2011.
[21]乔西波. 家兔GH、GHR及MSTN基因多态性及其与产肉性状的关系[D]. 泰安:山东农业大学,2010.
[22]Nair D,Ramesh V,Li R C,et al. Growth hormone releasing hormone (GHRH) signaling modulates intermittent hypoxia-induced oxidative stress and cognitive deficits in mouse[J]. Journal of Neurochemistry,2013,127(4):531-540.
[23]Meng Q Y,Chen Z Q,Yu Z Q,et al. Increased body weight via injecting myogenic expression growth hormone-releasing hormone (GHRH) plasmid DNA into sheep[J]. Animal Biotechnology,2004,15(2):175-192.
[24]Cheong H S,Yoon D H,Kim L H,et al. Growth hormone-releasing hormone (GHRH) polymorphisms associated with carcass traits of meat in Korean cattle[J]. BMC Genetics,2006,7:35.
[25]Johansson A,Jonasson I,Gyllensten U.Extended haplotypes in the growth hormone releasing hormone receptor gene (GHRHR) are associated with normal variation in height[J]. PLoS One,2009,4(2):e4464.
[26]张存芳.黄牛PTHRP、GHRH和GHRHR基因SNP及其与生长性状关联分析[D]. 杨凌:西北农林科技大学,2008.

相似文献/References:

[1]袁晓航,高剑峰,岳远瑞,等.绵羊基因组DNA甲基化与传染性胸膜肺炎的关联性分析[J].江苏农业科学,2013,41(10):24.
 Yuan Xiaohang,et al.Correlation analysis of sheep genomic DNA methylation and contagious pleuropneumonia[J].Jiangsu Agricultural Sciences,2013,41(8):24.
[2]要笑蕾,陈晓勇,孙洪新,等.寒泊羊与小尾寒羊繁殖性能及杂交效果分析[J].江苏农业科学,2019,47(04):131.
 Yao Xiaolei,et al.Analysis of reproductive performance and crossbreeding efficiency of Hanper mutton sheep and small-taied Han sheep[J].Jiangsu Agricultural Sciences,2019,47(8):131.
[3]刘琳玲,涂剑锋,郑军军,等.水貂GHR基因SNPs检测及其与生长性状的关联分析[J].江苏农业科学,2022,50(1):154.
 Liu Linling,et al.SNPs detection of GHR gene and its correlation with growth traits of mink[J].Jiangsu Agricultural Sciences,2022,50(8):154.
[4]何豫涵,卢小宁,陈景林,等.小尾寒羊GHR和GHRHR基因多态性与生长性状的关联分析[J].江苏农业科学,2024,52(4):187.
 He Yuhan,et al.Association analysis of GHR and GHRHR gene polymorphisms and growth traits in small-tailed Han sheep[J].Jiangsu Agricultural Sciences,2024,52(8):187.

备注/Memo

备注/Memo:
收稿日期:2022-08-17
基金项目:国家自然科学基金(编号:31201777);博士启动基金计划(编号:20111148);横向课题(编号:横20220047)。
作者简介:韩志松(1977—),男,辽宁阜新人,硕士,助理研究员,研究方向为畜牧生态模式。E-mail:hanzhisongcy@163.com。
通信作者,白俊艳,博士,副教授,研究方向为动物分子育种。E-mail:junyanbai@163.com。
更新日期/Last Update: 2023-04-20