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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:

第51卷

期数:

2023年第14期

页码:

70-75

栏目:

生物技术

出版日期:

2023-07-20

文章信息/Info

Title:

Establishment and application of RPA-LFD technique for rapid detection of bean yellow mosaic virus

作者:

朱宇翔¹; 2; 秦嘉超¹; 2; 季英华³; 陈新¹; 陈学好²; 崔晓艳¹

1.江苏省农业科学院经济作物研究所，江苏南京 210014； 2.扬州大学园艺与植物保护学院，江苏扬州 225009； 3. 江苏省农业科学院植物保护研究所，江苏南京 210014

Author(s):

Zhu Yuxiang; et al

关键词:

菜豆黄花叶病毒; 重组酶聚合酶扩增; 侧向流动试纸条; 病毒检测

Keywords:

-

分类号:

S436.43

DOI:

-

文献标志码:

A

摘要:

根据菜豆黄花叶病毒(bean yellow mosaic virus，简称BYMV)外壳蛋白基因序列的保守区设计特异性引物和探针，建立蚕豆中BYMV的重组酶聚合酶扩增(recombinase polymerase amplification，简称RPA)检测方法。同时，将该方法与侧向流动试纸条(LFD)检测方法相结合，建立RPA-LFD快速检测方法，并进行特异性和灵敏度验证。结果表明，该方法可在 37~42 ℃等温条件下进行，30 min即可完成检测。灵敏度试验表明，采用 RPA-LFD方法检测BYMV的灵敏性是PCR方法的 100 倍。在特异性试验方面，与同属于马铃薯Y病毒属亲缘关系较近的大豆花叶病毒(soybean mosaic virus，简称SMV)、菜豆普通花叶病毒(bean common mosaic virus，简称BCMV)和芜菁花叶病毒(turnip mosaic virus，简称TuMV)无交叉反应。因此，本研究建立的菜豆黄花叶病毒RPA-LFD技术检测快速、灵敏且高效，有望成为我国菜豆黄花叶病毒田间诊断与防控的实用性技术手段。

Abstract:

-

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相似文献/References:

备注/Memo

备注/Memo:

收稿日期：2022-10-08
基金项目：国家食用豆产业技术体系建设专项(编号：CARS-08-G15)；江苏现代农业(特粮特经)产业技术体系集成创新中心项目(编号：JATS［2019］399)。
作者简介：朱宇翔(1996—)，男，江苏南通人，硕士研究生，从事豆类作物病毒病的研究。E-mail：1017980413@qq.com。
通信作者：崔晓艳，博士，研究员，从事大豆花叶病毒及豆类作物抗病遗传育种研究，E-mail：cxy@jaas.ac.cn；陈学好，博士，教授，从事黄瓜品质性状和抗逆性状研究，E-mail：xhchen@yzu.edu.cn。

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更新日期/Last Update: 2023-07-20