|本期目录/Table of Contents|

[1]龚意辉,唐诗眙,周桂花,等.黄荆坪竹根椒叶绿体基因组密码子使用偏好性及影响因素分析[J].江苏农业科学,2023,51(20):28-34.
 Gong Yihui,et al.Codon usage bias of Capsicum annuum L. cv. Huangjingping chloroplast genome and its influence factors[J].Jiangsu Agricultural Sciences,2023,51(20):28-34.
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黄荆坪竹根椒叶绿体基因组密码子使用偏好性及影响因素分析(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第51卷
期数:
2023年第20期
页码:
28-34
栏目:
生物技术
出版日期:
2023-10-20

文章信息/Info

Title:
Codon usage bias of Capsicum annuum L. cv. Huangjingping chloroplast genome and its influence factors
作者:
龚意辉1唐诗眙1周桂花1陈磊1张斌1李鹏2
1.湖南人文科技学院农业与生物技术学院,湖南娄底 417000; 2.湘潭市农业科学研究所,湖南湘潭 411134
Author(s):
Gong Yihuiet al
关键词:
黄荆坪竹根椒叶绿体基因组密码子偏好性最优密码子
Keywords:
-
分类号:
S641.301
DOI:
-
文献标志码:
A
摘要:
为了解黄荆坪竹根椒(Capsicum annuum L. cv Huang Jingping)叶绿体基因组密码子使用模式,以其51条蛋白编码序列(coding sequence,CDS)为研究对象,采用Codon W 1.4.2、CUSP、 Excel 2010、SPSS16.0等软件对黄荆坪竹根椒密码子的GC含量、有效密码子值(ENC)、RSCU、中性绘图、ENC-plot、PR2-plot、最优密码子及其影响因素进行详细的分析。结果表明,黄荆坪竹根椒密码子第1位(GC1)、第2位(GC2)、第3位(GC3)中的GC含量分别为4268%、36.25%、27.89%,其中 GC1>GC2>GC3,表明密码子第3位碱基以A/U结尾为主;ENC值介于34.10%~5431%之间,平均值为46.55%,表明黄荆坪竹根椒密码子偏好性较弱。相关性分析结果表明:(1)GC1 与 GC2 呈极显著正相关,GCall与 GC1、GC2、GC3 均为极显著正相关;(2)ENC 与 GC1、GC2均不存在显著相关关系,但与 GC3 存在极显著相关关系。RSCU分析结果显示:在黄荆坪竹根椒叶绿体基因组中共有30个高频密码子的RSCU>1,其中以U、A、G结尾的高频密码子数分别为16、13、1个,表明黄荆坪竹根椒密码子第3位偏好以A或U结尾。ENC-plot绘图分析结果表明,黄荆坪竹根椒基因组中大多数基因位于标准曲线下方,有13个基因的ENC 比值介于-0.05~0.05 之间,说明选择压力主要影响黄荆坪竹根椒密码子的偏好性;PR2-plot 分析结果表明,黄荆坪竹根椒叶绿体基因组中大部分基因位于平面图的右下方,即 T>A、G>C,表明碱基突变、自然选择压力等多种因素共同影响黄荆坪竹根椒密码子的偏好性。在黄荆坪竹根椒叶绿体基因组中共筛选出GCA、GCU、AGA等19个最优密码子并全部以A/U结尾。本研究结果可为进一步开展黄荆坪竹根椒性状改良、遗传进化及叶绿体基因工程的应用提供分子理论支撑。
Abstract:
-

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备注/Memo

备注/Memo:
收稿日期:2022-12-22
基金项目:湖南省教育厅科学研究重点项目(编号:20A281);湖南省自然科学基金面上项目(编号:2021JJ30376);湖南省科技特派员服务乡村振兴项目(编号:2022NK4218);湘潭市农业科学研究所横向项目(编号:380220590112)。
作者简介:龚意辉(1988—),男,湖南涟源人,博士,讲师,主要从事园艺作物分子生物学方向研究。E-mail:gyhzgh@163.com。
通信作者:李鹏,农艺师,主要从事果蔬分子生物学方向研究。E-mail:907653797@qq.com。
更新日期/Last Update: 2023-10-20