|本期目录/Table of Contents|

[1]刘艳艳,丁颖,刘兴华,等.辣椒生长素抑制蛋白基因CaARP1的克隆及其对青枯菌侵染的响应[J].江苏农业科学,2023,51(21):13-19.
　Liu Yanyan,et al.Cloning of pepper auxin repressed protein gene CaARP1 and its response to Ralstonia solanacearum infection[J].Jiangsu Agricultural Sciences,2023,51(21):13-19.
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辣椒生长素抑制蛋白基因CaARP1的克隆及其对青枯菌侵染的响应(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第51卷
期数:
2023年第21期
页码:
13-19
栏目:
生物技术
出版日期:
2023-11-05

文章信息/Info

Title:
Cloning of pepper auxin repressed protein gene CaARP1 and its response to Ralstonia solanacearum infection
作者:
刘艳艳; 丁颖; 刘兴华; 郑佳秋
江苏沿海地区农业科学研究所，江苏盐城 224000
Author(s):
Liu Yanyan; et al
关键词:
辣椒; CaARP1基因; 基因克隆; 序列分析; 表达分析
Keywords:
-
分类号:
S641.301；S436.418.1+9
DOI:
-
文献标志码:
A
摘要:
生长素响应基因编码生长素抑制蛋白(ARP)，是非常重要的下调基因，能够抑制生长素(IAA)信号的转导，在植物的生长、发育、抗病、抗逆以及种子休眠等过程中发挥重要作用。为解析辣椒ARP1基因的序列特征和功能，以辣椒品种CM334为试材，克隆获得辣椒ARP1基因cDNA全长序列，命名为CaARP1，GenBank登录号为AAR83888.1。序列分析结果表明，辣椒CaARP1基因的cDNA全长228 bp，没有非翻译区，包含1个228 bp的开放阅读框架，编码75个氨基酸。CaARP1基因含有2个外显子和1个内含子，全长385 bp。CaARP1蛋白的分子量为8.298 ku，理论等电点为999，没有跨膜结构，不存在信号肽，为亲水性不稳定蛋白，二元结构元件多为无规则卷曲。CaARP1蛋白与同属茄科植物的马铃薯、番茄、黄果茄、烟草生长素抑制蛋白的同源性较高，序列一致性分别为95.00%、96.67%、93.24%、9189%。实时荧光定量PCR分析结果表明，CaARP1基因在青枯菌侵染1～7 d期间均呈现极显著下调的趋势，推测该基因可能在辣椒应答青枯病侵染中发挥重要作用。
Abstract:
-

参考文献/References:

［1］李成. 生长素抑制蛋白ARP1对烟草生长和抗病性的交叉调控作用［D］. 南京：南京农业大学，2015：1-5.
［2］刘艳艳，丁颖，郑佳秋，等. 植物PRRs和NLRs介导的免疫信号通路研究进展［J］. 江苏农业科学，2023，51(8)：43-50.
［3］Spoel S H，Dong X N.How do plants achieve immunity？ Defence without specialized immune cells［J］. Nature Reviews Immunology，2012，12(2)：89-100.
［4］Kepinski S，Leyser O.The Arabidopsis F-box protein TIR1 is an auxin receptor［J］. Nature，2005，435(7041)：446-451.
［5］Lee J，Han C T，Hur Y.Molecular characterization of the Brassica rapa auxin-repressed，superfamily genes，BrARP1 and BrDRM1［J］. Molecular Biology Reports，2013，40(1)：197-209.
［6］Gray W M，Kepinski S，Rouse D A，et al. Auxin regulates SCFTIR1-dependent degradation of AUX/IAA proteins［J］. Nature，2001，414(6861)：271-276.
［7］Kumar R，Agarwal P，Tyagi A K，et al. Genome-wide investigation and expression analysis suggest diverse roles of auxin-responsive GH3 genes during development and response to different stimuli in tomato (Solanum lycopersicum)［J］. Molecular Genetics and Genomics，2012，287(3)：221-235.
［8］Zhu Q，Li B Y，Mu S Y，et al. TTG2-regulated development is related to expression of putative auxin response factor genes in tobacco［J］. BMC Genomics，2013，14(1)：806.
［9］Hayashi K I.The interaction and integration of auxin signaling components［J］. Plant and Cell Physiology，2012，53(6)：965-975.
［10］Calderón Villalobos L I A，Lee S，de Oliveira C，et al. A combinatorial TIR1/AFB-Aux/IAA co-receptor system for differential sensing of auxin［J］. Nature Chemical Biology，2012，8(5)：477-485.
［11］Wang R H，Estelle M.Diversity and specificity：auxin perception and signaling through the TIR1/AFB pathway［J］. Current Opinion in Plant Biology，2014，21：51-58.
［12］Wu L M，Yu M，Holowachuk J，et al. Evaluation of a Brassica napus auxin-repressed gene induced by flea beetle damage and Sclerotinia sclerotiorum infection［J］. American Journal of Plant Sciences，2017，8(8)：1921-1952.
［13］Ulmasov T，Hagen G，Guilfoyle T J. ARF1，a transcription factor that binds to auxin response elements［J］. Science，1997，276(5320)：1865-1868.
［14］Abel S，Theologis A.Early genes and auxin action［J］. Plant Physiology，1996，111(1)：9-17.
［15］Guilfoyle T J，Hagen G.Auxin response factors［J］. Current Opinion in Plant Biology，2007，10(5)：453-460.
［16］Roosjen M，Paque S，Weijers D.Auxin response factors：output control in auxin biology［J］. Journal of Experimental Botany，2018，69(2)：179-188.
［17］Navarro L，Dunoyer P，Jay F，et al. A plant miRNA contributes to antibacterial resistance by repressing auxin signaling［J］. Science，2006，312(5772)：436-439.
［18］苏盼盼. 水稻生长素抑制基因OsARP1与Pid3介导的稻瘟病抗性关系的初步研究［D］. 南昌：南昌大学，2018：4-7.
［19］刘艳艳. CaSGT1和GaSRC2-1互作及其在PcINF1/CaSRC2-1激活辣椒免疫反应中的作用分析［D］. 福州：福建农林大学，2016.
［20］申磊. CaCDPK15、CaCBL1与CaCML13在辣椒应答青枯菌侵染或高温高湿胁迫中的作用分析［D］. 福州：福建农林大学，2020：3-27.
［21］Shen L，Liu Z Q，Yang S，et al. Pepper CabZIP63 acts as a positive regulator during Ralstonia solanacearum or high temperature-high humidity challenge in a positive feedback loop with CaWRKY40［J］. Journal of Experimental Botany，2016，67(8)：2439-2451.
［22］Livak K J，Schmittgen T D.Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCTmethod［J］. Methods，2001，25(4)：402-408.
［23］Zhao Y Y，Li C，Ge J，et al. Recessive mutation identifies auxin-repressed protein ARP1，which regulates growth and disease resistance in tobacco［J］. Molecular Plant-Microbe Interactions，2014，27(7)：638-654.
［24］Park S，Han K H.An auxin-repressed gene (RpARP) from black locust (Robinia pseudoacacia) is posttranscriptionally regulated and negatively associated with shoot elongation［J］. Tree Physiology，2003，23(12)：815-823.
［25］Zhou J M，Zhang Y L.Plant immunity：danger perception and signaling［J］. Cell，2020，181(5)：978-989.
［26］Wang Z X，Yang L Y，Jander G，et al. AIG2A and AIG2B limit the activation of salicylic acid-regulated defenses by tryptophan-derived secondary metabolism in Arabidopsis［J］. The Plant Cell，2022，34(11)：4641-4660.
［27］Bjornson M，Pimprikar P，Nürnberger T，et al. The transcriptional landscape of Arabidopsis thaliana pattern-triggered immunity［J］. Nature Plants，2021，7(5)：579-586.
［28］Macho A P，Schwessinger B，Ntoukakis V，et al. A bacterial tyrosine phosphatase inhibits plant pattern recognition receptor activation［J］. Science，2014，343(6178)：1509-1512.

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备注/Memo

备注/Memo:
收稿日期：2023-08-18
基金项目：江苏省农业科技自主创新资金［编号：CX(21)3031］；江苏省农业重大新品种创制专项(编号：PZCZ201715)。
作者简介：刘艳艳(1990—)，女，山东日照人，硕士，助理研究员，主要从事作物抗逆育种研究。E-mail：1052233980@qq.com。
通信作者：郑佳秋，硕士，副研究员，主要从事辣椒抗逆育种研究。E-mail：nky8236@163.com。
更新日期/Last Update: 2023-11-05