|本期目录/Table of Contents|

[1]邓文楷,彭艳,杨成,等.建兰MYB基因家族鉴定及在盐胁迫下的表达分析[J].江苏农业科学,2023,51(21):19-28.
 Deng Wenkai,et al.Identification of MYB gene family in Cymbidium ensifolium and its expression analysis under salt stress[J].Jiangsu Agricultural Sciences,2023,51(21):19-28.
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建兰MYB基因家族鉴定及在盐胁迫下的表达分析(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第51卷
期数:
2023年第21期
页码:
19-28
栏目:
生物技术
出版日期:
2023-11-05

文章信息/Info

Title:
Identification of MYB gene family in Cymbidium ensifolium and its expression analysis under salt stress
作者:
邓文楷1彭艳1杨成1李婷婷2潘贵英3
1.贵州民族大学生态环境工程学院,贵州贵阳 550025; 2.贵州民族大学研究生工作部,贵州贵阳 550025;3.贵州民族大学工程实训中心,贵州贵阳 550025
Author(s):
Deng Wenkaiet al
关键词:
建兰MYB转录因子盐胁迫基因组qRT-PCR
Keywords:
-
分类号:
S682.310.1
DOI:
-
文献标志码:
A
摘要:
植物MYB家族是最大的转录因子家族,在植物胁迫反应中起着重要作用。基于全基因组分析,对建兰(Cymbidium ensifolium)MYB家族成员进行鉴定和生物信息学分析,并分析相关基因在不同盐胁迫类型下的表达情况。结果表明,从建兰基因组中共鉴定出136个CeMYB转录因子,包括27个1R-MYB、102个R2R3-MYB、5个3R-MYB和2个4R-MYB。通过与拟南芥MYB进行系统发育得到20个基因簇,表明这些CeMYB可能具有多种生物学功能。亚细胞定位、染色体定位及保守域显示,136个CeMYB分布在18条染色体上,大多数CeMYB位于细胞核中,且具有典型的氨基酸序列重复;基序预测分析表明,多个保守元件主要位于CeMYB的N端,表明它们的功能相对保守。转录组表达分析表明,S13亚家族的CeMYB61和CeMYB89可能是调控盐胁迫的关键基因,qRT-PCR验证进一步表明,CeMYB61、CeMYB89在不同组织中表现为根系>假鳞茎>叶片>花序,其表达水平、模式与植株Na+浓度分布一致,证实CeMYB61、CeMYB89是建兰耐受盐胁迫的指示基因。研究为进一步探索MYB基因的潜在机制及盐胁迫下候选基因的挖掘提供了理论基础。
Abstract:
-

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备注/Memo

备注/Memo:
收稿日期:2023-05-13
基金项目:贵州省特色林业产业项目(编号:特林研2020-04);贵州省大学生创新创业项目(编号:S202010672021)。
作者简介:邓文楷(1996—),男,四川自贡人,硕士研究生,主要从事园艺环境工程研究。E-mail:dl18222235394@163.com。
通信作者:彭艳,博士,副教授,主要从事生物环境地球化学研究。E-mail:yan.jane.peng@ gmail.com。
更新日期/Last Update: 2023-11-05