|本期目录/Table of Contents|

[1]徐娅,黄佑岗,李树杏.水稻核糖体失活蛋白家族基因OsRIP8的生物信息学与功能分析[J].江苏农业科学,2024,52(4):71-79.
　Xu Ya,et al.Bioinformatics and functions analysis of ribosome-inactivating proteins (RIPs) family gene OsRIP8 in rice[J].Jiangsu Agricultural Sciences,2024,52(4):71-79.
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水稻核糖体失活蛋白家族基因OsRIP8的生物信息学与功能分析(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第52卷
期数:
2024年第4期
页码:
71-79
栏目:
生物技术
出版日期:
2024-02-20

文章信息/Info

Title:
Bioinformatics and functions analysis of ribosome-inactivating proteins (RIPs) family gene OsRIP8 in rice
作者:
徐娅; 黄佑岗; 李树杏
贵州省农业科学院水稻研究所，贵州贵阳 550006
Author(s):
Xu Ya; et al
关键词:
水稻; 核糖体失活蛋白; 生物信息学分析; 功能分析; 基因表达
Keywords:
-
分类号:
S511.01
DOI:
-
文献标志码:
A
摘要:
核糖体失活蛋白(RIPs)是一种能使真核细胞核糖体功能被抑制的毒蛋白。OsRIP8是属于该家族的一个功能未知基因。为研究该基因生物学功能，通过数据库对其进行生物信息学分析，利用qRT-PCR验证其表达模式，同时构建OsRIP8-RNAi 干涉载体并利用农杆菌转化水稻，采用PCR技术鉴定出转基因阳性植株，分析阳性植株的表达和农艺性状，初步揭示该基因的功能。结果表明，水稻OsRIP8蛋白为不稳定亲水蛋白，不含信号肽及完整的跨膜结构域，蛋白结构以α螺旋为主；在OsRIP8基因启动子区域存在着与植物生长发育、生物与非生物胁迫以及植物激素响应有关的元件；OsRIP8基因的共表达基因富集到多种大分子的生物合成与蛋白代谢等途径；qRT-PCR分析结果表明，OsRIP8基因在发育的小穗及抽穗前1 d的花药中优先表达；RNAi干涉后OsRIP8基因的表达下调造成植株花粉育性和结实率显著降低。暗示该基因对水稻小穗的发育具有重要作用，可能参与调控花粉发育过程。
Abstract:
-

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备注/Memo

备注/Memo:
收稿日期：2023-04-12
基金项目：贵州省科学技术基金(编号：黔科合基础［2020］1Z020)；贵州省农业科学院青年科技基金(编号：黔农科院青年基金［2019］01号)；贵州省科技计划(编号：黔科合支撑［2022］重点028号)；贵州省水稻研究所青年基金(编号：黔水稻所青年基金［2021］018号)。
作者简介：徐娅(1991—)，女，贵州贵阳人，硕士，助理研究员，研究方向为水稻生殖发育生物学。E-mail：xu_ya_1991@163.com。
通信作者：李树杏，硕士，正高级农艺师，研究方向为水稻栽培生理学。E-mail：24406882@qq.com。
更新日期/Last Update: 2024-03-20