|本期目录/Table of Contents|

[1]刘丽丽,陈银霞,聂蔚丹,等.LRR-RLKs参与番茄抗根结线虫病途径中的功能分析与验证[J].江苏农业科学,2024,52(4):63-71.
　Liu Lili,et al.Functional analysis and verification of LRR RLKs in tomato defense against root-knot nematode[J].Jiangsu Agricultural Sciences,2024,52(4):63-71.
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LRR-RLKs参与番茄抗根结线虫病途径中的功能分析与验证(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第52卷
期数:
2024年第4期
页码:
63-71
栏目:
生物技术
出版日期:
2024-02-20

文章信息/Info

Title:
Functional analysis and verification of LRR RLKs in tomato defense against root-knot nematode
作者:
刘丽丽; 陈银霞; 聂蔚丹; 冯成蒿; 王超楠; 杨中敏; 杜崇
新疆农业大学园艺学院，新疆乌鲁木齐830052
Author(s):
Liu Lili; et al
关键词:
LRR-RLK; 番茄; 抗病; 根结线虫; VIGS
Keywords:
-
分类号:
S436.412
DOI:
-
文献标志码:
A
摘要:
前期以对南方根结线虫(Meloidogyne incognita)具有热稳定抗性的野生秘鲁番茄LA3858(Mi-3/Mi-3)为试材，采用不同土壤温度处理形成抗感态，进行转录组测序(RNA-seq)。本研究以该测序数据为依托，筛选出8个编码LRR-RLKs的差异表达基因(DEGs)并进行相关分析与功能验证。结果表明，目标LRR-RLKs基因分布在番茄的1号、2号、3号、5号、7号和8号染色体上，编码蛋白均被定位于细胞膜上；在目标LRR-RLKs中，只有编码Solyc07g0558103的氨基酸是碱性氨基酸；组织特异性表达分析发现，Solyc05g056370.3在栽培番茄Heinz1706根部较其他基因高水平表达，并且接种线虫后，该基因在LA3858抗病材料根部响应迅速，24 h内积累水平明显高于其他基因；同时，系统进化树分析发现，Solyc05g056370.3所在分组大部分蛋白与油菜素类信号传导、细胞死亡控制、发病机制等免疫防卫进程相关；进一步VIGS功能验证揭示，Solyc05g056370.3正向调控番茄对根结线虫的抗性。综上，Solyc05g056370.3参与番茄介导抗病并正反馈响应防卫进程，是很有价值的抗性改良资源，因此，该基因的深入挖掘对后续番茄抗根结线虫病新材料的选育具有理论指导意义。
Abstract:
-

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备注/Memo

备注/Memo:
收稿日期：2023-05-28
基金项目：新疆维吾尔自治区自然科学基金青年基金(编号：2022D01B95)；2020年新疆维吾尔自治区“天池博士计划”项目(编号：390000017)；新疆维吾尔自治区重点研发计划子课题(编号：2022B02032-2)。
作者简介：刘丽丽(1998—)，女，河南周口人，硕士研究生，研究方向为番茄分子遗传育种。E-mail：206346559@qq.com。
通信作者：杜崇，博士，硕士生导师，主要从事番茄分子遗传育种研究。E-mail：godv2018@163.com。
更新日期/Last Update: 2024-03-20