|本期目录/Table of Contents|

[1]张婷,游小英.产铁载体的根际促生菌鉴定及其对月季生长和养分吸收的影响[J].江苏农业科学,2024,52(4):174-181.
 Zhang Ting,et al.Identification of rhizobacteria producing siderophores and their effects on growth and nutrient absorption of Rosa chinensis[J].Jiangsu Agricultural Sciences,2024,52(4):174-181.
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产铁载体的根际促生菌鉴定及其对月季生长和养分吸收的影响(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第52卷
期数:
2024年第4期
页码:
174-181
栏目:
园艺与林学
出版日期:
2024-02-20

文章信息/Info

Title:
Identification of rhizobacteria producing siderophores and their effects on growth and nutrient absorption of Rosa chinensis
作者:
张婷游小英
上海农林职业技术学院风景园林技术系,上海 201699
Author(s):
Zhang Tinget al
关键词:
假单胞菌产铁载体月季地上部根系生长养分吸收嗜铁素
Keywords:
-
分类号:
S685.120.6;S182
DOI:
-
文献标志码:
A
摘要:
以月季蓝丝带根际土为试材,采用铬天青S(CAS)琼脂平板测定法对根际促生菌进行分离,利用平板划线法对菌株进行分离提纯,分析不同分离菌株的促生效果和铁吸收能力,并探索铁载体物质对月季矿质元素吸收的影响。结果表明,通过CAS琼脂培养基共获得3株分离株(SP1、SP2、SP3),16S rRNA分子鉴定显示,SP1、SP2、SP3均属于假单胞菌属(Pseudomonas),分别为铜绿假单胞菌(P. aeruginosa)、喜昆虫假单胞菌(P. entomophila)、恶臭假单胞菌(P. putida)。其中,SP3促生效果、铁含量较佳,铁载体活性可达87.20%。此外,SP3可分泌嗜铁素将不溶性 Fe(OH)3 转化为可溶性铁,提高根系及地上部铁含量,且显著影响月季幼苗对其他元素(K、P、Ca、Mg、Zn、Mn)的吸收。综上,从月季根际分离得到的1株恶臭假单胞菌(SP3)具有较佳的产铁载体活性,可促进植株生长、铁含量及矿质养分吸收,或可作为开发高效菌肥的潜在资源。
Abstract:
-

参考文献/References:

[1]Liang G.Iron uptake,signaling,and sensing in plants[J]. Plant Communications,2022,3(5):100349.
[2]张林琳,刘星星,祝亚昕,等. 机理Ⅰ植物铁营养的吸收转运及信号调控机制研究进展[J]. 植物营养与肥料学报,2021,27(7):1258-1272.
[3]Ning X Y,Lin M F,Huang G H,et al. Research progress on iron absorption,transport,and molecular regulation strategy in plants[J]. Frontiers in Plant Science,2023,14:1190768.
[4]温佳辛,王超林,冯慧,等. 月季花色研究进展[J]. 园艺学报,2021,48(10):2044-2056.
[5]李晓彬,康跃虎. 滨海重度盐碱地微咸水滴灌水盐调控及月季根系生长响应研究[J]. 农业工程学报,2019,35(11):112-121.
[6]Jiao X,He G,Cui Z,et al. Agri-environment policy for grain production in China:toward sustainable intensification[J]. China Agricultural Economic Review,2018,10(1):78-92.
[7]张建鹏. 化肥减量配施微生物菌肥及土壤调理剂对重茬马铃薯生长发育和土壤质量的影响[J]. 江苏农业科学,2023,51(7):205-212.
[8]周益帆,白寅霜,岳童,等. 植物根际促生菌促生特性研究进展[J]. 微生物学通报,2023,50(2):644-666.
[9]刘晓婷,姚拓,马亚春,等. 植物根际促生菌发酵液中植物激素含量的液液萃取-高效液相色谱测定法[J]. 草业科学,2022,39(10):2222-2228.
[10]黄靖,陈婵. 接种促生菌对金线莲生物活性成分及土壤细菌群落的影响[J]. 江苏农业科学,2022,50(23):184-191.
[11]李文祥,张天谣,贾颜,等. 含ACC脱氨酶的根际促生菌对切花月季生长及生理的影响[J]. 山东农业科学,2022,54(4):42-48.
[12]彭雯杰,詹伊婧,雷鹏,等. 阿斯青霉菌XK-12产铁载体特性及其抑菌活性[J]. 江苏农业学报,2022,38(1):73-80.
[13]Delaporte-Quintana P,Lovaisa N C,Rapisarda V A,et al. The plant growth promoting bacteria Gluconacetobacter diazotrophicus and Azospirillum brasilense contribute to the iron nutrition of strawberry plants through siderophores production[J]. Plant Growth Regulation,2020,91(2):185-199.
[14]Gu S H,Wei Z,Shao Z Y,et al. Competition for iron drives phytopathogen control by natural rhizosphere microbiomes[J]. Nature Microbiology,2020,5(8):1002-1010.
[15]Lurthy T,Cantat C,Jeudy C,et al. Impact of bacterial siderophores on iron status and ionome in pea[J]. Frontiers in Plant Science,2020,11:730.
[16]田洁,唐有林,王启璋,等. 植物根际促生菌Pseudomonas putida UW4对菊花扦插苗生长及生理特性的影响[J]. 植物生理学报,2023,59(1):179-190.
[17]葛淼淼,薄永琳,刘宸,等. 土壤产铁载体细菌的筛选及其对铁氧化物的活化与利用[J]. 微生物学通报,2023,50(3):1062-1072.
[18]Hoegy F,Mislin G L A,Schalk I J.Pyoverdine and pyochelin measurements[M]//Pseudomonas methods and protocols.New York:Humana,2014:293-301.
[19]Trapet P,Avoscan L,Klinguer A,et al. The Pseudomonas fluorescens siderophore pyoverdine weakens Arabidopsis thaliana defense in favor of growth in iron-deficient conditions[J]. Plant Physiology,2016,171(1):675-693.
[20]刘金涛,姚凡,李臻园,等. 植物铁素吸收机制研究进展[J]. 热带农业科学,2022,42(5):26-33.
[21]张文静,程建峰,刘婕,等. 植物铁素(Fe)营养的生理研究进展[J]. 中国农学通报,2021,37(36):103-110.
[22]Pang Z Q,Chen J A,Wang T H,et al. Linking plant secondary metabolites and plant microbiomes:a review[J]. Frontiers in Plant Science,2021,12:621276.
[23]Duan Y N,Chen R,Zhang R,et al. Isolation,identification,and antibacterial mechanisms of Bacillus amyloliquefaciens QSB-6 and its effect on plant roots[J]. Frontiers in Microbiology,2021,12:746799.
[24]Zhuang L B,Li Y,Wang Z S,et al. Synthetic community with six Pseudomonas strains screened from garlic rhizosphere microbiome promotes plant growth[J]. Microbial Biotechnology,2021,14(2):488-502.
[25]Mridha S,Kümmerli R.Coordination of siderophore gene expression among clonal cells of the bacterium Pseudomonas aeruginosa[J]. Communications Biology,2022,5:545.
[26]Liu D,Yang Q Q,Ge K,et al. Promotion of iron nutrition and growth on peanut by Paenibacillus illinoisensis and Bacillus sp.strains in calcareous soil[J]. Brazilian Journal of Microbiology,2017,48(4):656-670.
[27]王亚楠,陈莹莹,吴玉洪,等. 甲基营养型芽孢杆菌对黄瓜促生作用及其机理研究[J]. 北方园艺,2020(12):1-7.
[28]刘邮洲,沈佳慧,乔俊卿,等. 芽孢杆菌嗜铁素研究进展[J]. 江苏农业学报,2023,39(1):266-276.
[29]刘邮洲,沈佳慧,乔俊卿,等. 芽孢杆菌嗜铁素研究进展[J]. 江苏农业学报,2023,39(1):266-276.
[30]Zhao L,Wang Y,Kong S.Effects of Trichoderma asperellum and its siderophores on endogenous auxin in Arabidopsis thaliana under iron-deficiency stress[J]. International Microbiology,2020,23(4):501-509.
[31]Jin C W,Li G X,Yu X H,et al. Plant Fe status affects the composition of siderophore-secreting microbes in the rhizosphere[J]. Annals of Botany,2010,105(5):835-841.
[32]常竣泊,马哲宇,丁忠杰,等. 植物种子铁储存、运输和再利用分子机制的研究进展[J]. 浙江大学学报(农业与生命科学版),2021,47(4):473-480.
[33]Coleto I,Marín-Pea A J,Urbano-Gámez J A,et al. Interaction of ammonium nutrition with essential mineral cations[J]. Journal of Experimental Botany,2023,74(19):6131-6144.

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备注/Memo

备注/Memo:
收稿日期:2023-09-14
基金项目:上海市现代农业产业技术体系项目(编号:KY2-0000-21-05)。
作者简介:张婷(1987—),女,江苏丹阳人,硕士,讲师,主要从事园林植物栽培养护研究工作。E-mail:tingz280026@163.com。
通信作者:游小英,硕士,副教授,主要从事园林植物培育研究工作。E-mal:xyyoush@163.com。
更新日期/Last Update: 2024-03-20