|本期目录/Table of Contents|

[1]朱桃,张双玉,张路阳,等.枯草芽孢杆菌4种环脂肽抗猪δ冠状病毒的研究[J].江苏农业科学,2024,52(4):182-186，192.
　Zhu Tao,et al.Study on resistance of four cyclic lipopeptides of Bacillus subtilis against porcine delta coronavirus[J].Jiangsu Agricultural Sciences,2024,52(4):182-186，192.
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枯草芽孢杆菌4种环脂肽抗猪δ冠状病毒的研究(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第52卷
期数:
2024年第4期
页码:
182-186，192
栏目:
畜牧兽医与水产蚕桑
出版日期:
2024-02-20

文章信息/Info

Title:
Study on resistance of four cyclic lipopeptides of Bacillus subtilis against porcine delta coronavirus
作者:
朱桃1; 2; 张双玉1; 2; 张路阳1; 钟纯燕3; 陈悦雯1; 李基棕2; 李彬2; 罗楚平1
1.淮阴工学院，江苏淮安 223002； 2.江苏省农业科学院兽医研究所，江苏南京 210014；3.黔西南民族职业技术学院生物工程系，贵州兴义 562400
Author(s):
Zhu Tao; et al
关键词:
表面活性素; 罗克霉素; 泛革素; 杆菌霉素L; 猪δ冠状病毒
Keywords:
-
分类号:
S852.65+1
DOI:
-
文献标志码:
A
摘要:
为探究从枯草芽孢杆菌Bs 916中提取的表面活性素、罗克霉素、泛革素及杆菌霉素L 4种抗菌肽对猪δ冠状病毒(PDCoV)的抗病毒作用。首先采用CCK-8法确定抗菌肽对细胞无毒性的最佳浓度范围，随后分别采用RT-qPCR、Western blot及IFA 3种方法检测Bs 916中4种环脂肽对PDCoV的抗病毒效果。结果显示，4种环脂肽在浓 度≤50 μg/mL时对细胞无毒性，同时具有良好的抗病毒效果。相同条件下，罗克霉素抗病毒效果最佳，其次为表面活性素。本研究从枯草芽孢杆菌Bs 916中提取的表面活性素、罗克霉素、泛革素及杆菌霉素L可抑制PDCoV在细胞中的增殖，具有良好的抗病毒作用，其中以罗克霉素和表面活性素的抗病毒效果最佳，可为抗PDCoV药物研发提供理论支持。
Abstract:
-

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相似文献/References:

[1]王皓楠,靳鹏飞,康迅,等.解淀粉芽孢杆菌HAB-6抑菌物质及其相关基因的分析[J].江苏农业科学,2018,46(04):79.
　Wang Haonan,et al.Analysis of Bacillus amyloliquefaciens HAB-6 inhibitory substance and its related genes[J].Jiangsu Agricultural Sciences,2018,46(4):79.

备注/Memo

备注/Memo:
收稿日期：2023-05-17
基金项目：江苏省重点研发计划(编号：BE2021324)；江苏省农业自主创新资金(编号：［CX(21)3127］)。
作者简介：朱桃(1994—)，女，河南商丘人，硕士研究生，主要从事非洲猪瘟疫苗研究。E-mail：1694490993@qq.com。
通信作者：李彬，博士，研究员，主要从事动物腹泻病防控技术等研究，E-mail：libinana@126.com；罗楚平，博士，教授，主要从事微生物代谢产物研究，E-mail：luochuping@163.com。
更新日期/Last Update: 2024-03-20