[1]付瑞敏,韩鸿鹏,张丽琴,等.葡萄霜霉病和白粉病拮抗菌的分离、鉴定和He-Ne 激光诱变[J].江苏农业科学,2013,41(08):122-125.
　Fu Ruimin,et al.Isolation and identification of antagonistic bacteria against grape downy mildew and powdery mildew，and its mutation under He-Ne laser irradiation[J].Jiangsu Agricultural Sciences,2013,41(08):122-125.
点击复制

葡萄霜霉病和白粉病拮抗菌的分离、鉴定和He-Ne 激光诱变()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第41卷
期数:
2013年08期
页码:
122-125
栏目:
植物保护
出版日期:
2013-08-25

文章信息/Info

Title:
Isolation and identification of antagonistic bacteria against grape downy mildew and powdery mildew，and its mutation under He-Ne laser irradiation
作者:
付瑞敏1; 2;  韩鸿鹏1;  张丽琴1;  张红1;  常慧萍1;  邢文会1;  杨雪1;  刘春雷1;  王丁1;  陈五岭2
1.河南教育学院，河南郑州 450046； 2.西北大学，陕西西安 710069
Author(s):
Fu Ruimin; et al
关键词:
葡萄; 霜霉病; 白粉病; 拮抗菌; 激光 诱变
Keywords:
-
分类号:
Q933
DOI:
-
文献标志码:
A
摘要:
从葡萄果园土壤中筛选出可有效抑制葡萄致病菌葡萄白粉病病原菌和葡萄霜霉病病原菌生长的菌株，通过表型生理学、生物化学研究，筛选出的菌株PT4被鉴定为枯草芽孢杆菌。为了增强PT4菌株的拮抗能力，对其进行 He-Ne 激光诱变，获得6株突变株，通过拮抗力和遗传稳定性的检测，对葡萄白粉病病原菌和葡萄霜霉病病原菌拮抗性最强的突变株PT46被挑选出来。根据试验结果，认为He-Ne 激光诱变拮抗菌的方法可以应用于葡萄霜霉病和葡萄白粉病的生物防治领域。
Abstract:
-

参考文献/References:

［1］Condit R，Hubbell S P. Abundance and DNA sequence of two-base repeat regions in tropical tree genomes［J］. Genome，1991，34(1)：66-71.
［2］Dai G H，Andary C，Cosson-Mondolot L，et al. Polyphenols and resistance of grapevine to downy mildew［J］. Acta Horticulturae，1994(381)：763-766.
［3］刘会宁，李华. 欧亚种葡萄对白粉病与霜霉病的抗性研究［J］. 东北农业大学学报，2004，35(3)：302-308.
［4］Shoda M. Bacterial control of plant disease［J］. Biosci Bioeng，2000，89(6)：515-521.
［5］Pamela G M. An effective biofungicide with novel modes of action［J］. Pesticide Outlook，2002，13(5)：193-194.
［6］Yu G，Jia X，Wen J，et al. Strain improvement of Streptomyces roseosporus for daptomycin production by rational screening of He-Ne laser and NTG induced mutants and kinetic modeling［J］. Applied Biochemistry and Biotechnology，2011，163(6)：729-743.
［7］Fani R，Bandi C，Bazzicalupo M，et al. Phylogeny of the genus Azospirillum based on 16S rDNA sequence［J］. FEMS Microbiology Letters，1995，129(2/3)：195-200.
［8］Han J，Sun L，Dong X，et al. Characterization of a novel plant growth-promoting bacteria strain Delftia tsuruhatensis HR4 both as a diazotroph and a potential biocontrol agent against various plant pathogens［J］. Systematic and Applied Microbiology，2005，28(1)：66-76.

相似文献/References:

[1]芮东明,刘亚柏,刘吉祥,等.句容市葡萄产业现状及葡萄栽培技术特点[J].江苏农业科学,2013,41(04):136.
[2]毛妮妮,刘照亭,刘伟忠,等.观赏性葡萄开屏式培植技术[J].江苏农业科学,2014,42(12):224.
　Mao Nini,et al.Spreading tail cultivation techniques of ornamental grapes[J].Jiangsu Agricultural Sciences,2014,42(08):224.
[3]刘伟忠,毛妮妮,郭建,等.醉金香葡萄的引种表现及省力化栽培技术[J].江苏农业科学,2015,43(12):199.
　Liu Weizhong,et al.Introduction performance and laborsaving cultural techniques of “Zuijinxiang” grape[J].Jiangsu Agricultural Sciences,2015,43(08):199.
[4]汪纯凤,王勇,陈英,等.抗葡萄白腐病菌放线菌株的筛选、纯化与鉴定[J].江苏农业科学,2015,43(12):166.
　Wang Chunfeng,et al.Screening，purification and identification of Actinomycetes with resistance to Coniothyrium diplodiella[J].Jiangsu Agricultural Sciences,2015,43(08):166.
[5]陈剑侠.葡萄叶片和果实对硒的吸收和累积特性[J].江苏农业科学,2013,41(07):154.
　Chen Jianxia.Absorption and accumulation of selenium in leaves and fruits of grape during different growth stages[J].Jiangsu Agricultural Sciences,2013,41(08):154.
[6]吕良玉,温晓蕾,杨娜娜,等.葡萄生长后期不同叶位与霜霉病发病级下叶片的光合特性[J].江苏农业科学,2013,41(09):141.
　Lv Liangyu,et al.Photosynthetic characteristics of leaves with different leaf positions and grades of downy mildew in late growth stage of grape[J].Jiangsu Agricultural Sciences,2013,41(08):141.
[7]钱亚明,吴伟民,赵密珍,等.江苏5个葡萄试验示范基地土壤肥力状况调查分析[J].江苏农业科学,2013,41(09):145.
　Qian Yaming,et al.Investigation and analysis of soil fertility in five grape pilot demonstration bases of Jiangsu Province[J].Jiangsu Agricultural Sciences,2013,41(08):145.
[8]王西成,钱亚明,赵密珍,等.江苏地区设施葡萄常用品种需冷量研究[J].江苏农业科学,2013,41(10):120.
　Wang Xicheng,et al.Study on chilling requirements of common grape varieties cultivated in greenhouse in Jiangsu Province[J].Jiangsu Agricultural Sciences,2013,41(08):120.
[9]李鹏程,郭绍杰,李铭,等.新型复混调节剂对葡萄生长及果实性状的影响[J].江苏农业科学,2013,41(12):166.
　Li Pengcheng,et al.Effects of new compound modifier on growth and fruit characters of Vitis amurensis Rupr.[J].Jiangsu Agricultural Sciences,2013,41(08):166.
[10]曹婷,朱明,丁莎莎,等.茶多酚复配剂对新美人指葡萄贮藏品质的影响[J].江苏农业科学,2016,44(05):336.
　Cao Ting,et al.Effect of tea polyphenols complex agent on preservation of “New Manicure Finger” grape[J].Jiangsu Agricultural Sciences,2016,44(08):336.
[11]李宝燕,王英姿,刘学卿,等.3种杀菌剂对葡萄霜霉病菌的毒力测定和田间药效试验[J].江苏农业科学,2014,42(01):98.
　Li Baoyan,et al.Toxicity tests and field control effects of three fungicides against grape downy mildew pathogen[J].Jiangsu Agricultural Sciences,2014,42(08):98.
[12]巩培杰,康军,包若煊,等.葡萄霜霉菌株的收集及侵染12个葡萄品种后症状差异分析[J].江苏农业科学,2024,52(4):149.
　Gong Peijie,et al.Collection of grape downy mildew strains and analysis of symptom differences after infecting 12 grape varieties[J].Jiangsu Agricultural Sciences,2024,52(08):149.

备注/Memo

备注/Memo:
收稿日期：2013-05-13
基金项目：河南省科技攻关重点项目(编号122102310171)；陕西省重大科技创新项目(编号2009ZKC04-16)；河南教育学院青年科研项目(编号20100103)。
作者简介：付瑞敏(1981—)，女，河南郑州人，博士研究生，讲师，从事农业及食品微生物研究。E-mail：angelaminmin@163.com。
通信作者：陈五岭，教授，博士生导师，从事农业、环境及食品微生物研究。E-mail：wuling.chen@263.net。
更新日期/Last Update: 2013-08-25