[1]李倩,赵振军,崔龙波,等.MG132对小鼠卵母细胞排卵后老化的调控作用[J].江苏农业科学,2013,41(11):217-220.
　Li Qian,et al.Regulatory role of MG132 on aging of post-ovulatory mouse oocytes[J].Jiangsu Agricultural Sciences,2013,41(11):217-220.
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MG132对小鼠卵母细胞排卵后老化的调控作用()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第41卷
期数:
2013年11期
页码:
217-220
栏目:
畜牧兽医
出版日期:
2013-11-25

文章信息/Info

Title:
Regulatory role of MG132 on aging of post-ovulatory mouse oocytes
作者:
李倩1; 2;  赵振军1;  崔龙波1;  石慧1;  刘晓玲1;  王敏强1
1.烟台大学生命科学学院，山东烟台 264005； 2.山东农业大学动物科技学院，山东泰安 271018
Author(s):
Li Qian; et al
关键词:
小鼠; 卵母细胞; MG132; 老化; 皮质颗粒; BCL2
Keywords:
-
分类号:
A
DOI:
-
文献标志码:
Q492
摘要:
为了研究蛋白酶体抑制剂MG132对小鼠卵母细胞排卵后老化的调控作用，找到抑制卵母细胞老化的方案，通过孤雌激活和胚胎发育试验系统研究了MG132在不同温度下对小鼠卵母细胞老化的调控作用，并通过体外受精发育以及细胞免疫组化试验，分别研究了15 ℃时MG132对卵母细胞受精后胚胎发育能力、皮质颗粒分布与BCL2蛋白水平的影响。结果表明，15 ℃时卵母细胞在添加1 μmol/L MG132的HCZB中培养12 h，然后在37 ℃恢复6 h，是筛选的抑制卵母细胞老化的优化方案，说明一定低温联合适当浓度的蛋白酶体抑制
Abstract:
-

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期：2013-07-24
基金项目：山东省自然科学基金(编号：ZR2011CQ003)。
作者简介：李倩(1980—)，女，山东临沂人，博士，讲师，主要研究方向为动物细胞工程。E-mail：ydskyshx@163.com。
更新日期/Last Update: 2013-11-25