牦牛胃蛋白酶原C基因的克隆及序列分析-《江苏农业科学》

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Title:: Cloning and sequence analysis of yak pepsinogen C gene

作者:: 朱小翌¹; 2; 张林¹; 2; 骆美蓉²; 3; 江伟华¹; 2; 林忠荔¹; 2; 刘益丽¹; 2; 江明锋¹; 2; 1.动物遗传育种学国家民委-教育部共建重点实验室四川成都 610041； 2.西南民族大学青藏高原研究院，四川成都 610041；3.青藏高原动物遗传资源保护与利用四川省重点实验室，四川成都 610041

Author(s):: Zhu Xiaoyi; et al

关键词:: 牦牛; PGC基因; 克隆; 序列分析

Keywords:: -

分类号:: A

DOI:: -

文献标志码:: TS201.2+5；Q785

摘要:: 采用RT-PCR技术，成功获得了牦牛胃蛋白酶原C基因，用ProtParam在线工具对牦牛胃PGC蛋白的基本性质进行测定。结果表明，其相对分子质量为42 931.6，pI值为4.45，半衰期为30 h，不稳定系数为40.76，总平均亲水性为0.041。对该蛋白的二级、三级结构分析表明，牦牛胃蛋白酶结构与黄牛没有明显区别，均含有5个α-螺旋、25个β-折叠，三维结构也基本一致。

Abstract:: -

参考文献/References:

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