[1]孙晓梅,黄金光.禾谷镰刀菌甾醇14α脱甲基酶基因cDNA克隆及生物信息学分析[J].江苏农业科学,2016,44(03):31-36.
　Sun Xiaomei,et al.cDNA cloning and bioinformatics analysis of sterol 14α-demethylase gene in Fusarium graminearum[J].Jiangsu Agricultural Sciences,2016,44(03):31-36.
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禾谷镰刀菌甾醇14α脱甲基酶基因cDNA克隆
及生物信息学分析()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第44卷
期数:
2016年03期
页码:
31-36
栏目:
生物技术
出版日期:
2016-03-25

文章信息/Info

Title:
cDNA cloning and bioinformatics analysis of sterol 14α-demethylase gene in Fusarium graminearum
作者:
孙晓梅1;  黄金光2
1.青岛农业大学动漫与传媒学院，山东青岛 266109；
2.山东省植物病虫害综合防控重点实验室/青岛农业大学农学与植物保护学院，山东青岛 266109
Author(s):
Sun Xiaomei; et al
关键词:
禾谷镰刀菌; 甾醇14α脱甲基酶; 基因克隆; 抗药性; 生物信息学分析; cDNA
Keywords:
-
分类号:
S182；Q785
DOI:
-
文献标志码:
A
摘要:
克隆获得禾谷镰刀菌(Fusarium graminearum)2个甾醇14α脱甲基酶CYP51蛋白(CYP51A、CYP51B)基因cDNA序列，明确其氨基酸序列典型特征，为研究蛋白质结构及其抗药性机制奠定基础。采用RT-PCR技术，克隆2个CYP51蛋白基因cDNA序列，利用相关生物信息软件对其序列进行生物信息学分析。结果克隆到2条cDNA序列，长度分别为1 524、1 581 bp，分别编码507、526个氨基酸；2个蛋白分子量分别为57.5、59.3 ku，等电点分别为6
Abstract:
-

参考文献/References:

［1］张洪滨，柳金伟，刘秉江，等. 山东省小麦赤霉病菌种群组成及其致病力分化［J］. 植物保护学报，2013，40(1)：27-32.
［2］Dean R，van Kan J A，Pretorius Z A，et al. The top 10 fungal pathogens in molecular plant pathology［J］. Molecular Plant Pathology，2012，13(4)：414-430.
［3］龚双军，杨立军，向礼波，等. 2013年湖北省小麦赤霉病菌对多菌灵和戊唑醇的敏感性［J］. 农药学学报，2014，16(5)：610-613.
［4］Alcazar-Fuoli L，Mellado E，Garcia-Effron G，et al. Ergosterol biosynthesis pathway in Aspergillus fumigatus［J］. Steroids，2008，73(3)：339-347.
［5］Bean T P，Cools H J，Lucas J A，et al. Sterol content analysis suggests altered eburicol 14α-demethylase (CYP51) activity in isolates of Mycosphaerella graminicola adapted to azole fungicides［J］. FEMS Microbiol Lett，2009，296(2)：266-273.
［6］Lepesheva G I，Waterman M R. Sterol 14α-demethylase cytochrome P450(CYP51)，a P450 in all biological kingdoms［J］. Biochim Biophys Acta，2007，1770(3)：467-477.
［7］Ghosoph J M，Schmidt L S，Margosan D A，et al. Imazalil resistance linked to a unique insertion sequence in the PdCYP51 promoter region of Penicillium digitatum［J］. Postharvest Biology and Technology，2007，44(1)：9-18.
［8］Mellado E，Garcia-Effron G，Alcázar-Fuoli L，et al. A new Aspergillus fumigatus resistance mechanism conferring in vitro cross-resistance to azole antifungals involves a combination of cyp51A alterations［J］. Antimicrobial Agents and Chemotherapy，2007，51(6)：1897-1904.
［9］Snelders E，van der Lee H A L，Kuijpers J，et al. Emergence of azole resistance in Aspergillus fumigatus and spread of a single resistance mechanism［J］. PLoS Medicine，2008，5(11)：e219.
［10］Kudo M，Ohi M，Aoyama Y，et al. Effects of Y132H and F145L substitutions on the activity，azole resistance and spectral properties of Candida albicans sterol 14-demethylase P450 (CYP51)：a live example showing the selection of altered P450 through interaction with environmental compounds［J］. Journal of Biochemistry，2005，137(5)：625-632.
［11］Park H G，Lee I S，Chun Y J，et al. Heterologous expression and characterization of the sterol 14α-demethylase CYP51F1 from Candida albicans［J］. Archives of Biochemistry and Biophysics，2011，509(1)：9-15.
［12］Becher R，Wirsel S G. Fungal cytochrome P450 sterol 14α-demethylase (CYP51) and azole resistance in plant and human pathogens［J］. Applied Microbiology and Biotechnology，2012，95(4)：825-840.
［13］Morio F，Loge C，Besse B，et al. Screening for amino acid substitutions in the Candida albicans Erg11 protein of azole-susceptible and azole-resistant clinical isolates：new substitutions and a review of the literature［J］. Diagnostic Microbiology and Infectious Disease，2010，66(4)：373-384.
［14］Caas-Gutiérrez G P，Angarita-Velásquez M J，Restrepo-Flórez J M，et al. Analysis of the CYP51 gene and encoded protein in propiconazole-resistant isolates of Mycosphaerella fijiensis［J］. Pest Management Science，2009，65(8)：892-899.
［15］Wyand R A，Brown J K. Sequence variation in the CYP51 gene of Blumeria graminis associated with resistance to sterol demethylase inhibiting fungicides［J］. Fungal Genetics and Biology，2005，42(8)：726-735.
［16］Leroux P，Walker A S. Multiple mechanisms account for resistance to sterol 14α-demethylation inhibitors in field isolates of Mycosphaerella graminicola［J］. Pest Management Science，2011，67(1)：44-59.
［17］Stammler G，Cordero J，Koch A，et al. Role of the Y134F mutation in cyp51 and overexpression of cyp51 in the sensitivity response of Puccinia triticina to epoxiconazole［J］. Crop Protection，2009，28(10)：891-897.
［18］Mullins J G L，Parker J E，Cools H J，et al. Molecular modelling of the emergence of azole resistance in Mycosphaerella graminicola［J］. PLoS One，2011，6(6)：e20973.
［19］Podust L M，Stojan JPoulos T L，Waterman M R. Substrate recognition sites in 14α-sterol demethylase from comparative analysis of amino acid sequences and X-ray structure of Mycobacterium tuberculosis CYP51［J］. J Inorg Biochem，2001，87(4)：227-235.
［20］Cools H J，Fraaije B A. Update on mechanisms of azole resistance in Mycosphaerella graminicola and implications for future control［J］. Pest Management Science，2013，69(2)：150-155.
［21］Lepesheva G I，Waterman M R. Structural basis for conservation in the CYP51 family［J］. Biochimica et Biophysica acta，2011，1814(1)：88-93.
［22］Fan J，Urban M，Parker J E，et al. Characterization of the sterol 14α-demethylases of Fusarium graminearum identifies a novel genus-specific CYP51 function［J］. The New Phytologist，2013，198(3)：821-835.
［23］Cuomo C A，Güldener U，Xu J R，et al. The fusarium graminearum genome reveals a Link between localized polymorphism and pathogen specialization［J］. Science，2007，317(5843)：1400-1402.
［24］Gasteiger E，Hoogland C，Gattiker A，et al. The proteomics protocols handbook［M］. Totowa，New Jersey：Humana Press，2005：571-607.
［25］Marchler-Bauer A，Lu S，Anderson J B，et al. CDD：a conserved domain database for the functional annotation of proteins［J］. Nucleic Acids Research，2011，39：D225-D229.
［26］Aoyama Y，Noshiro M，Gotoh O，et al. Sterol 14-demethylase P450 (P45014DM*) is one of the most ancient and conserved P450 species［J］. Journal of Biochemistry，1996，119(5)：926-933.
［27］Werck-Reichhart D，Feyereisen R. Cytochromes P450：a success story［J］. Genome Biology，2000，1(6)：1-9.
［28］Becher R，Weihmann F，Deising H B，et al. Development of a novel multiplex DNA microarray for Fusarium graminearum and analysis of azole fungicide responses［J］. BMC Genomics，2011，12(1)：52.

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备注/Memo

备注/Memo:
收稿日期：2015-03-11
基金项目：国家自然科学基金(编号：31471735)；国家科技基础条件平台子项目(编号：2005DKA21207-17)；山东省“泰山学者”建设工程专项经费(编号：TSSD201316)；青岛农业大学高层次人才科研基金(编号：631217)。
作者简介：孙晓梅(1975—)，女，山东莱州人，硕士，副教授，主要从事生物信息学研究。E-mail：xmsun@qau.edu.cn。
通信作者：黄金光，博士，教授，主要从事蛋白质结构生物学、分子植物病理学研究。E-mail：jghuang@qau.edu.cn。
更新日期/Last Update: 2016-03-25