[1]凌善锋,蔡福欢,刘彦文,等.3.5 mg/L水杨酸诱导斜生栅藻积累虾青素的分子机理[J].江苏农业科学,2016,44(04):287-290.
　Ling Shanfeng,et al.Molecular mechanism of astaxanthin accumulation of Scenedesmus obliquus induced by salicylic acid[J].Jiangsu Agricultural Sciences,2016,44(04):287-290.
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3.5 mg/L水杨酸诱导斜生栅藻积累虾青素
的分子机理()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第44卷
期数:
2016年04期
页码:
287-290
栏目:
畜牧兽医与水产蚕桑
出版日期:
2016-04-25

文章信息/Info

Title:
Molecular mechanism of astaxanthin accumulation of Scenedesmus obliquus induced by salicylic acid
作者:
凌善锋;  蔡福欢;  刘彦文;  朱勇;  韩梁;  程淼
荆楚理工学院生物工程学院，湖北荆门 448000
Author(s):
Ling Shanfeng; et al
关键词:
水杨酸; 斜生栅藻; 形态; 生物量; 虾青素; 分子机制
Keywords:
-
分类号:
S917
DOI:
-
文献标志码:
A
摘要:
在自然条件下，向对数生长期的斜生栅藻(Scenedesmus obliquus)培养液中加入3.5 mg/L的水杨酸溶液，进行胁迫培养以诱导藻类细胞内虾青素的合成与积累。在培养过程中，每隔3 d对藻液取样制片，然后用显微镜观察其生长状况及细胞形态变化。结果发现：经3.5 mg/L水杨酸诱导的藻的形态发生了明显变化，该水杨酸溶液对于斜生柵藻细胞来说是一种逆境胁迫因子，藻细胞内通过积累高达1.123 mg/g的虾青素来形成一种自我保护机制。八氢番茄红素脱氢酶(PDS)和β-胡萝卜素加氧酶(crtO)基因表达水平上调是藻细胞合成虾青素的主要调控机制。随着培养时间增加，藻细胞生物量呈时间依赖性增加(除15 d外)，最高达2.08×106个/mL。
Abstract:
-

参考文献/References:

［1］凌善锋. 天然虾青素产业的发展趋势分析［J］. 生物学通报，2014，49(1)：6-7.
［2］Qin S，Liu G X，Hu Z Y. The accumulation and metabolism of astaxanthin in Scenedesmus obliquus(Chlorophyceae)［J］. Process Biochemistry，2008，43： 795-802.
［3］高政权，孟春晓，刁英英.施用水杨酸对雨生红球藻中虾青素积累的影响［J］. 水产科学，2007，26(7)：377-380.
［4］Sanchez-Camargo A P，Meireles M，Ferreira A L，et al. Extraction of ω-3 fatty acids and astaxanthin from Brazilian redspotted shrimp waste using supercritical CO2 ethanol mixtures［J］. The Journal of Supercritical Fluids，2012，61(1)：71-77.
［5］凌善锋.雨生红球藻培养基和诱导条件优化［J］. 江苏农业科学，2013，41(11)：266-267.
［6］Gao Z Q，Meng C X，Zhang X W，et al. Induction of salicylic acid (SA) on transcriptional expression of eight carotenoid genes and astaxanthin accumulation in Haematococcus pluvialis［J］. Enzyme and Microbial Technology，2012，51(4)： 225-230.
［7］Lu Y D，Jiang P，Liu S F，et al. Methyl jasmonate- or gibberellins A3-induced astaxanthin accumulation is associated with up-regulation of transcription of beta-carotene ketolase genes (bkts) in microalga Haematococcus pluvialis［J］. Bioresource Technology，2010，101(16)： 6468-6474.
［8］Vidhyavathi R，Venkatachalam L，Sarada R，et al. Regulation of carotenoid biosynthetic genes expression and carotenoid accumulation in the green alga Haematococcus pluvialis under nutrient stress conditions［J］. Journal of Experimental Botany，2008，59(6)： 1409-1418.
［9］Gao Z Q，Meng C X，Chen Y C，et al. Comparison of astaxanthin accumulation and biosynthesis gene expression of three Haematococcus pluvialis strains upon salinity stress［J］. Journal of Applied Phycology，2015，27(5)：1853-1860.
［10］陈兴才，欧阳琴，黄亚治. 雨生红球藻物理破壁法提取虾青素研究［J］. 中国食品学报，2007，7(2)：48-52.
［11］姜淼，杨贤庆，李来好，等. 内源酶辅助提取虾壳虾青素的研究［J］. 南方水产科学，2011，7(2)：55-60.
［12］Raman V，Ravi S. Effect of salicylic acid and methyl jasmonate on antioxidant systems of Haematococcus pluvialis［J］. Acta Physiologiae Plantarum，2011，33(3)：1043-1049.
［13］Jin E，Lee C G，Polle J E. Secondary carotenoid accumulation in Haematococcus (Chlorophyceae)： Biosynthesis，regulation，and biotechnology［J］. Journal of Microbiology and Biotechnology，2006，16(6)： 821-831.
［14］Huang Jun Chao，Chen Feng，Sandmann G. Stress-related differential expression of multiple beta-carotene ketolase genes in the unicellular green alga Haematococcus pluvialis［J］. Journal of Biotechnology，2006，122(2)： 176-185.
［15］凌善锋. 产虾青素的斜生栅藻藻种的培育［J］. 湖北农业科学，2014，53(2)：283-285.

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备注/Memo

备注/Memo:
收稿日期：2015-11-22
基金项目：国家自然科学基金(编号：30671394)；药物合成与优化湖北省重点实验室开放基金(编号：OPP2014YB02)；湖北省教育厅科学技术研究项目(编号：Q20126101)；荆楚理工学院引进博士科研启动经费 (编号：2013BK05)；湖北省教育厅省属高校青年教师出国留学项目(编号：鄂教外函［2014］59号)。
通信作者简介：凌善锋(1971—)，男，浙江富阳人，博士，副教授，主要从事天然虾青素的产业化研究工作。E-mail：shanfeng86@163.com。
更新日期/Last Update: 2016-04-25