[1]唐会会,郑玉才,王鹏非,等.牦牛PRDX5基因的克隆测序及其在牦牛与犏牛睾丸中的表达差异[J].江苏农业科学,2016,44(08):53-56.
　Tang Huihui,et al.Cloning and sequencing of PRDX5 gene in yak and its differential expression in testes of yak and sterile hybrid cattle-yak[J].Jiangsu Agricultural Sciences,2016,44(08):53-56.
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牦牛PRDX5基因的克隆测序
及其在牦牛与犏牛睾丸中的表达差异()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第44卷
期数:
2016年08期
页码:
53-56
栏目:
生物技术
出版日期:
2016-08-25

文章信息/Info

Title:
Cloning and sequencing of PRDX5 gene in yak and its differential expression in testes of yak and sterile hybrid cattle-yak
作者:
唐会会;  郑玉才;  王鹏非;  金素钰;  黄林
西南民族大学生命科学与技术学院，四川成都 610041
Author(s):
Tang Huihui; et al
关键词:
牦牛; 杂交雄性不育; PRDX5基因; 基因表达; 睾丸
Keywords:
-
分类号:
Q785；S823.8
DOI:
-
文献标志码:
A
摘要:
测定牦牛PRDX5基因序列，并比较其在牦牛和雄性不育犏牛睾丸组织中的表达差异，以探究该基因与犏牛雄性不育的关系。从牦牛睾丸组织中提取总RNA，采用RT-PCR技术克隆并测序获得牦牛PRDX5基因的cDNA序列；利用实时荧光定量RT-PCR技术检测该基因在牦牛与犏牛睾丸组织中的表达情况。结果表明：克隆获得的牦牛Prdx5序列长759 bp，包含长660 bp的CDS区，该序列与普通牛基因相差4个碱基，序列同源性为99.47%，相差的4个核苷酸导致推导的氨基酸序列存在3个氨基酸残基差异；Prdx5在牦牛和犏牛睾丸组织中均有表达，在牦牛睾丸组织中的表达量极显著高于犏牛(P<0.01)，是犏牛睾丸组织中表达量的6倍，提示Prdx5在犏牛睾丸组织中低水平表达可能与其雄性不育有关。
Abstract:
-

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备注/Memo

备注/Memo:
收稿日期：2015-06-24
基金项目：国家自然科学基金(编号：31240053)。
作者简介：唐会会(1995—)，女，贵州六盘水人。E-mail：18224022329@163.com。
通信作者：黄林，硕士，讲师，主要从事动物生物化学与分子遗传学研究。E-mail：huanglin200426@hotmail.com。
更新日期/Last Update: 2016-08-25