|本期目录/Table of Contents|

[1]赵盼盼,王丽,袁园园,等.提高CRISPR/Cas9系统靶向编辑效率方法的研究进展[J].江苏农业科学,2017,45(01):12-15.
 Zhao Panpan,et al.Research progress on methods of enhancing CRISPR/Cas9 system target editing effects[J].Jiangsu Agricultural Sciences,2017,45(01):12-15.
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提高CRISPR/Cas9系统靶向编辑效率方法的研究进展(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第45卷
期数:
2017年01期
页码:
12-15
栏目:
专论与综述
出版日期:
2017-01-05

文章信息/Info

Title:
Research progress on methods of enhancing CRISPR/Cas9 system target editing effects
作者:
赵盼盼 王丽 袁园园 常卫东 王林嵩
河南师范大学生命科学学院,河南新乡 453007
Author(s):
Zhao Panpanet al
关键词:
CRISPR/Cas9系统向导RNA脱靶效应农作物
Keywords:
-
分类号:
S188
DOI:
-
文献标志码:
A
摘要:
近年来,CRISPR/Cas9系统经过一系列改造后已成为继锌指核酸酶ZFNs和TALENs后的新型高效定点编辑的新技术,目前,该技术已成功应用于人类细胞、斑马鱼、小鼠以及细菌的基因组精确编辑,但是该技术在农作物等植物中的应用还比较受限,且其脱靶效应等问题还有待解决。本文首先简要综述了CRISPR/Cas9系统的发展历程、结构组成和作用机制及其在农作物中的应用,进而综述了近年来探索出的提高CRISPR/Cas9系统靶向编辑效率的方法。最后,对基因组编辑技术在农作物和作物育种上的应用进行了展望。
Abstract:
-

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相似文献/References:

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[2]杨海峰,甘晓雪,薄高峰,等.利用CRISPR/Cas9系统编辑银腺杨84K LAZY基因[J].江苏农业科学,2022,50(18):258.
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备注/Memo

备注/Memo:
收稿日期:2016-07-08
基金项目:国家自然科学基金(编号:31601241);河南省高等学校重点科研项目(编号:17A180007);河南师范大学博士启动课题(编号:qd14172)。
作者简介:赵盼盼(1991—),女,河南濮阳人,硕士研究生,研究方向为植物抗逆。E-mail:zhaoppan1504183002@163.com。
通信作者:王林嵩,博士,教授。E-mail:wls@htu.cn。
更新日期/Last Update: 2017-01-05