[1]梁宇尧,钟昌桦,潘若云,等.百香果CBF基因家族鉴定与表达分析[J].江苏农业科学,2024,52(10):55-61.
 Liang Yuyao,et al.Identification and expression analysis of CBF gene family in Passiflora edulis[J].Jiangsu Agricultural Sciences,2024,52(10):55-61.
点击复制

百香果CBF基因家族鉴定与表达分析()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第52卷
期数:
2024年第10期
页码:
55-61
栏目:
生物技术
出版日期:
2024-05-20

文章信息/Info

Title:
Identification and expression analysis of CBF gene family in Passiflora edulis
作者:
梁宇尧钟昌桦潘若云任锐方庭
福建农林大学园艺学院/福建农林大学园艺植物遗传育种研究所,福建福州 350002
Author(s):
Liang Yuyaoet al
关键词:
百香果CBF基因低温胁迫表达分析
Keywords:
-
分类号:
S667.901
DOI:
-
文献标志码:
A
摘要:
百香果(Passiflora edulis Sims)原产热带地区,对低温十分敏感。CBF(C-repeat binding factor)基因家族是AP2转录因子家族的一类成员,在植物应对低温、干旱和盐等非生物胁迫中发挥重要作用。对百香果CBF基因家族进行鉴定和分析,并探究其在低温胁迫下的表达模式,可为进一步研究百香果CBF基因功能提供重要的参考信息。采用生物信息学方法鉴定百香果CBF基因家族成员,并对家族成员的序列组分、理化性质、系统进化关系、染色体定位、基因结构、保守基序、启动子顺式作用元件和转录因子结合位点等进行分析,通过qRT-PCR方法分析其在低温胁迫下的表达模式。结果显示,百香果基因组中共有5个CBF基因家族成员,其中3个分布在1号染色体上,2个分布在7号染色体上,被预测定位到细胞核、质膜和过氧化物酶体中;含有1~2个外显子,11~12个motif;系统进化显示5个百香果CBF基因、10个水稻CBF基因及6个拟南芥CBF基因共分成了2大亚组:亚组Ⅰ中包括拟南芥的全部6个CBF基因、百香果的全部5个CBF基因和水稻的4个CBF基因,水稻剩下的6个CBF基因分到了亚组Ⅱ中;启动子区域含有25种顺式作用元件,以光响应、激素与逆境胁迫响应元件为主;共有20个转录因子结合位点;3个百香果CBF基因在低温胁迫下表达量极显著上升,推测上述基因参与了百香果低温胁迫响应。
Abstract:
-

参考文献/References:

[1]刘紫烟,刘佳乐,朱圆圆,等. 木本植物低温应答机制研究进展[J]. 西北林学院学报,2022,37(2):1-7.
[2]Agarwal P K,Agarwal P,Reddy M K,et al. Role of DREB transcription factors in abiotic and biotic stress tolerance in plants[J]. Plant Cell Reports,2006,25(12):1263-1274.
[3]Jaglo K R,Kleff S,Amundsen K L,et al. Components of the Arabidopsis C-repeat/dehydration-responsive element binding factor cold-response pathway are conserved in Brassica napus and other plant species[J]. Plant Physiology,2001,127(3):910-917.
[4]Gilmour S J,Sebolt A M,Salazar M P,et al. Overexpression of the Arabidopsis CBF3 transcriptional activator mimics multiple biochemical changes associated with cold acclimation[J]. Plant Physiology,2000,124(4):1854-1865.
[5]Zhang X,Fowler S G,Cheng H M,et al. Freezing-sensitive tomato has a functional CBF cold response pathway,but a CBF regulon that differs from that of freezing-tolerant Arabidopsis[J]. The Plant Journal:for Cell and Molecular Biology,2004,39(6):905-919.
[6]解盼,刘蔚,康郁,等. 甘蓝型油菜CBF基因家族的鉴定和表达分析[J]. 作物学报,2021,47(12):2394-2406.
[7]Cook D,Fowler S,Fiehn O,et al. A prominent role for the CBF cold response pathway in configuring the low-temperature metabolome of Arabidopsis[J]. Proceedings of the National Academy of Sciences of the United States of America,2004,101(42):15243-15248.
[8]Savitch L V,Allard G,Seki M,et al. The effect of overexpression of two Brassica CBF/DREB1-like transcription factors on photosynthetic capacity and freezing tolerance in Brassica napus[J]. Plant and Cell Physiology,2005,46(9):1525-1539.
[9]Gilmour S J,Zarka D G,Stockinger E J,et al. Low temperature regulation of the Arabidopsis CBF family of AP2 transcriptional activators as an early step in cold-induced COR gene expression[J]. The Plant Journal,1998,16(4):433-442.
[10]Thomashow M F. Molecular basis of plant cold acclimation:insights gained from studying the CBF cold response pathway[J]. Plant Physiology,2010,154(2):571-577.
[11]Haake V,Cook D,Riechmann J,et al. Transcription factor CBF4 is a regulator of drought adaptation in Arabidopsis[J]. Plant Physiology,2002,130(2):639-648.
[12]Lehti-Shiu M D,Uygun S,Moghe G D,et al. Molecular evidence for functional divergence and decay of a transcription factor derived from whole-genome duplication in Arabidopsis thaliana[J]. Plant Physiology,2015,168(4):1717-1734.
[13]Dubouzet J G,Sakuma Y,Ito Y,et al. OsDREB genes in rice,Oryza sativa L.,encode transcription activators that function in drought-,high-salt- and cold-responsive gene expression[J]. The Plant Journal,2003,33(4):751-763.
[14]刘龙博,郑树轩,郑洁. 石榴低温响应因子CBF基因家族鉴定及其表达分析[J]. 西北农业学报,2022,31(9):1154-1167.
[15]贾会霞,李建波,孙佩,等. 胡杨CBF基因家族的鉴定及表达特性分析[J]. 分子植物育种,2017,15(2):492-500.
[16]隋晓燕,胡日生,晏伟杰,等. 烟草CBF基因全基因组鉴定及响应低温胁迫表达分析[J]. 分子植物育种,2023,21(3):799-808.
[17]牛先前,江莉,林秋金,等. 福建百香果果实品质优势区域划分[J]. 中国农学通报,2023,39(7):40-45.
[18]Ma D N,Dong S S,Zhang S C,et al. Chromosome-level reference genome assembly provides insights into aroma biosynthesis in passion fruit (Passiflora edulis)[J]. Molecular Ecology Resources,2021,21(3):955-968.
[19]Munhoz C F,Santos A A,Arenhart R A,et al. Analysis of plant gene expression during passion fruit-Xanthomonas axonopodis interaction implicates lipoxygenase 2 in host defence[J]. Annals of Applied Biology,2015,167(1):135-155.
[20]Livak K J,Schmittgen T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method[J]. Methods,2001,25(4):402-408.
[21]Medina J,Bargues M,Terol J,et al. The Arabidopsis CBF gene family is composed of three genes encoding AP2 domain-containing proteins whose expression is regulated by low temperature but not by abscisic acid or dehydration[J]. Plant Physiology,1999,119(2):463-470.
[22]汪泽文,杨依维,王鹏飞,等. 欧李DREB基因家族的鉴定与分析[J]. 植物生理学报,2020,56(3):413-422.
[23]Castillon A,Shen H,Huq E. Phytochrome interacting factors:central players in phytochrome-mediated light signaling networks[J]. Trends in Plant Science,2007,12(11):514-521.
[24]Leivar P,Monte E,Oka Y,et al. Multiple phytochrome-interacting bHLH transcription factors repress premature seedling photomorphogenesis in darkness[J]. Current Biology,2008,18(23):1815-1823.
[25]Kami C,Lorrain S,Hornitschek P,et al. Light-regulated plant growth and development[J]. Current Topics in Developmental Biology,2010,91:29-66.
[26]Jiang B C,Shi Y T,Zhang X Y,et al. PIF3 is a negative regulator of the CBF pathway and freezing tolerance in Arabidopsis[J]. Proceedings of the National Academy of Sciences of the United States of America,2017,114(32):E6695-E6702.
[27]李丽容,杨凯,陈惠,等. 百香果寒冻害低温等级指标研究[J]. 自然灾害学报,2023,32(1):191-198.
[28]Yang X Y,Wang R,Jing H H,et al. Three novel C-repeat binding factor genes of Dimocarpus longan regulate cold stress response in Arabidopsis[J]. Frontiers in Plant Science,2020,11:1026.

相似文献/References:

[1]包雨莹,彭先蕊,潘若云,等.百香果SUS基因家族鉴定与表达分析[J].江苏农业科学,2024,52(11):45.
 Bao Yuying,et al.Identification and expression analysis of SUS gene family in Passiflora edulis[J].Jiangsu Agricultural Sciences,2024,52(10):45.
[2]张小英,李嘉昱,王叶,等.微生物菌剂对百香果生长发育及果实产量品质的影响[J].江苏农业科学,2024,52(13):155.
 Zhang Xiaoying,et al.Influences of microbial agents on growth, fruit yield and quality of Passiflora edulis[J].Jiangsu Agricultural Sciences,2024,52(10):155.
[3]王叶,张小英,陈彩霞,等.有机肥配施微生物菌剂对百香果土壤及果实品质的影响[J].江苏农业科学,2026,54(3):181.
 Wang Ye,et al.Influences of organic fertilizer combined with microbial agent on soil and fruit quality of passion fruit[J].Jiangsu Agricultural Sciences,2026,54(10):181.

备注/Memo

备注/Memo:
收稿日期:2023-07-22
基金项目:福建省自然科学基金(编号:2021J05022);福建省种业创新与产业化工程项目(编号:zycxny2021010);福建省高原学科建设经费(编号:102/71201801101)。
作者简介:梁宇尧(1998—),男,山西阳泉人,硕士研究生,从事果树遗传育种方面的研究。E-mail:670915524@qq.com。
通信作者:方庭,博士,副教授,从事果树遗传育种方面的研究。E-mail:fangting@fafu.edu.cn。
更新日期/Last Update: 2024-05-20