[1]包雨莹,彭先蕊,潘若云,等.百香果SUS基因家族鉴定与表达分析[J].江苏农业科学,2024,52(11):45-51.
 Bao Yuying,et al.Identification and expression analysis of SUS gene family in Passiflora edulis[J].Jiangsu Agricultural Sciences,2024,52(11):45-51.
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百香果SUS基因家族鉴定与表达分析()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第52卷
期数:
2024年第11期
页码:
45-51
栏目:
生物技术
出版日期:
2024-06-05

文章信息/Info

Title:
Identification and expression analysis of SUS gene family in Passiflora edulis
作者:
包雨莹彭先蕊潘若云任锐方庭
福建农林大学园艺学院/福建农林大学园艺植物遗传育种研究所,福建福州 350002
Author(s):
Bao Yuyinget al
关键词:
百香果蔗糖合酶高温胁迫表达分析
Keywords:
-
分类号:
S667.901
DOI:
-
文献标志码:
A
摘要:
百香果(Passiflora edulis Sims)虽原产热带地区,但高温也会影响其开花坐果,进而影响产量。果实糖含量不仅影响果实风味品质,还可以作为渗透调节物质参与高温、干旱、盐害等逆境胁迫。蔗糖合成酶(sucrose synthase,SUS)是蔗糖代谢的关键酶之一,在植物糖积累及非生物胁迫响应中具有重要功能。对百香果SUS基因家族成员进行鉴定和生物信息学分析,并分析其在低温胁迫下的表达变化。运用生物信息学方法,对百香果蔗糖合成酶基因进行全基因组鉴定与基因结构、系统发育关系、蛋白质保守功能域、染色体定位分析,利用转录组数据分析其表达模式,利用qRT-PCR技术分析其在高温处理下的表达。结果显示,百香果中包含5个SUS基因,分布在4条染色体上,外显子数量为13~15个。5个SUS基因家族成员可分为三大类,即SUSⅠ、SUSⅡ、SUSⅢ,百香果SUS基因家族成员启动子中含有激素响应、逆境响应相关的顺式作用元件。表达模式分析表明,PeSUS3基因在百香果叶片和果皮中的表达量均较高。qRT-PCR分析表明,PeSUS1、PeSUS2基因在高温处理后表达量显著下降,PeSUS5基因表达量显著性上升,说明PeSUS1、PeSUS2、PeSUS5基因可能在百香果高温胁迫中发挥重要的调控作用。
Abstract:
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参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2023-07-27
基金项目:福建省自然科学基金(编号:2021J05022);福建省种业创新与产业化工程项目(编号:zycxny2021010);福建省高原学科建设经费(编号:102/71201801101)。
作者简介:包雨莹(1999—),女,浙江台州人,硕士研究生,主要从事果树遗传育种方面的研究。E-mail:304492734@qq.com。
通信作者:方庭,博士,副教授,主要从事果树遗传育种方面的研究。E-mail:fangting@fafu.edu.cn。
更新日期/Last Update: 2024-06-05