[1]张馨怡,郭宝佃,韩晨阳,等.猕猴桃溃疡病菌Ⅲ型效应蛋白HopAH1的三维结构与生化功能分析[J].江苏农业科学,2024,52(12):121-127.
 Zhang Xinyi,et al.Three-dimensional structure and biochemical function analysis of HopAH1, a type Ⅲ effector protein of Pseudomonas syringae pv. actinidiae[J].Jiangsu Agricultural Sciences,2024,52(12):121-127.
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猕猴桃溃疡病菌Ⅲ型效应蛋白HopAH1的三维结构与生化功能分析()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第52卷
期数:
2024年第12期
页码:
121-127
栏目:
植物保护
出版日期:
2024-06-20

文章信息/Info

Title:
Three-dimensional structure and biochemical function analysis of HopAH1, a type Ⅲ effector protein of Pseudomonas syringae pv. actinidiae
作者:
张馨怡1郭宝佃2韩晨阳3赵延存2刘凤权12
1.海南大学热带农林学院,海南海口 570228; 2.江苏省农业科学院植物保护研究所,江苏南京 210014; 3.南京农业大学园艺学院,江苏南京 210095
Author(s):
Zhang Xinyiet al
关键词:
猕猴桃溃疡病菌效应蛋白三维结构生化功能
Keywords:
-
分类号:
S436.634.1+9
DOI:
-
文献标志码:
A
摘要:
猕猴桃溃疡病危害严重,影响猕猴桃的产量。对猕猴桃溃疡病菌14个保守的Ⅲ型效应蛋白进行了功能域预测分析,发现HopAH1属于糖基水解酶超级家族。搜索同源蛋白并结合进化树分析发现,HopAH1与丁香假单胞菌番茄致病变种的效应蛋白亲缘关系较近,而与其他同源蛋白的亲缘关系相对较远。Alpha Fold2 预测三维结构发现,HopAH1具有糖基水解酶GH5家族典型的桶状结构。酶活性测定试验发现HopAH1不能水解槐树豆胶、羧甲纤维素钠以及对硝基苯基-β-D半乳糖苷等3种底物,推测可能存在其他特异性底物。使用农杆菌介导的蛋白瞬时表达体系在本氏烟中表达HopAH1,发现不能引起烟草细胞坏死。研究结果将为HopAH1的作用机制研究提供参考。
Abstract:
-

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2023-12-14
基金项目:国家自然科学基金(编号:32202261);江苏省自然科学基金(编号:BK20210156)。
作者简介:张馨怡(1997—),女,陕西咸阳人,硕士研究生,从事植物病理学研究。E-mail:zhangxinyi0302@163.com。
通信作者:郭宝佃,博士,助理研究员,从事植物与病原互作研究,E-mail:guo_baodian@163.com;刘凤权,博士,教授,从事植物细菌病害研究,E-mail:fqliu20011@sina.com。
更新日期/Last Update: 2024-06-20