|本期目录/Table of Contents|

[1]陈坤,刘亚利,覃宏宇,等.基于SNP标记的玉米散粉吐丝间隔期QTL定位[J].江苏农业科学,2024,52(16):79-86.
 Chen Kun,et al.QTL mapping of maize anthesis silking interval based on SNP markers[J].Jiangsu Agricultural Sciences,2024,52(16):79-86.
点击复制

基于SNP标记的玉米散粉吐丝间隔期QTL定位(PDF)
分享到:

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第52卷
期数:
2024年第16期
页码:
79-86
栏目:
生物技术
出版日期:
2024-08-20

文章信息/Info

Title:
QTL mapping of maize anthesis silking interval based on SNP markers
作者:
陈坤1刘亚利1覃宏宇1周锦国1弓雪1韦正乙1钟昌松2杨耀迥1卢生乔1张述宽2
1.广西壮族自治区农业科学院玉米研究所,广西南宁 530007; 2.广西壮族自治区农业科学院,广西南宁 530007
Author(s):
Chen Kunet al
关键词:
玉米散粉吐丝间隔期SNP分子标记QTL
Keywords:
-
分类号:
S513.032
DOI:
-
文献标志码:
A
摘要:
散粉吐丝间隔期(ASI)性状是玉米耐旱性的重要指标之一,短ASI有利于提高玉米受精成功率从而提高籽粒产量,挖掘玉米ASI主效QTL对于选育高产、耐旱型玉米品种有重要意义。选择ASI间隔期长的自交系ZHF141和ASI间隔期短的自交系GH701构建204份F2、F2∶3家系为试验材料,在试验田重复种植不同家系并调查每株ASI天数,获得群体表型数据。利用分布全基因组的10K玉米SNP分子标记筛选多态性获得4 755个分子标记,过滤偏分离后得到2 478个分子标记,采用2种软件构建遗传图谱,软件QTL IciMapping v 4.2去除冗余标记后得到 1 807 个分子标记用于构建遗传图谱,软件JoinMap 4去除相似标记后得到2 084个分子标记用于构建遗传图谱。采用2种软件检测ASI性状QTL位点,软件QTL IciMapping v4.2用1 807个分子标记和表型数据共检测到4个QTL位点,分别位于第2、4、5、5染色体上,软件MapQTL用2 084个分子标记和表型数据共检测到6个QTL位点,分别位于第2、2、4、5、6、6染色体上,2种软件检测到调控玉米ASI性状的主效QTL均在第2染色体上,有部分QTL置信区间是相同的。本试验结果认为玉米ASI性状主效QTL位于第2染色体上。
Abstract:
-

参考文献/References:

[1]李少昆,赵久然,董树亭,等. 中国玉米栽培研究进展与展望[J]. 中国农业科学,2017,50(11):1941-1959.
[2]刘成,申海兵,石云素,等. 水分胁迫后玉米雌雄穗开花间隔时间(ASI)与产量和抗旱性的关系研究[J]. 新疆农业科学,2008,45(4):609-612.
[3]Almeida G D,Makumbi D,Magorokosho C,et al. QTL mapping in three tropical maize populations reveals a set of constitutive and adaptive genomic regions for drought tolerance[J]. Theoretical and Applied Genetics,2013,126(3):583-600.
[4]Ribaut J M,Hoisington D A,Deutsch J A,et al. Identification of quantitative trait loci under drought conditions in tropical maize.1.Flowering parameters and the anthesis-silking interval[J]. Theoretical and Applied Genetics,1996,92(7):905-914.
[5]Li X H,Liu X D,Li M S,et al. Identification of quantitative trait loci for anthesis-silking interval and yield components under drought stress in maize[J]. Acta Botanica Sinica,2003,45(7):852-857.
[6]王迪,李永祥,王阳,等. 基于两个相关群体的玉米花期相关性状QTL定位[J]. 中国农业科学,2010,43(13):2633-2644.
[7]Agrama H A S,Moussa M E. Mapping QTLs in breeding for drought tolerance in maize (Zea mays L.)[J]. Euphytica,1996,91(1):89-97.
[8]Zhu J J,Wang X P,Sun C X,et al. Mapping of QTL associated with drought tolerance in a semi-automobile rain shelter in maize (Zea mays L.)[J]. Agricultural Sciences in China,2011,10(7):987-996.
[9]Hu X M,Wang G H,Du X M,et al. QTL analysis across multiple environments reveals promising chromosome regions associated with yield-related traits in maize under drought conditions[J]. The Crop Journal,2021,9(4):759-766.
[10]侯清桂,张君,田磊,等. 基于SNP标记连锁图谱的玉米花期性状QTL定位[J]. 玉米科学,2021,29(6):41-49.
[11]杨媛,刘亚苹,王建华,等. 联合QTL定位和转录组分析揭示玉米开花期的遗传基础[J]. 玉米科学,2023,31(1):40-47.
[12]陈俊宇,卢峰,陈婉莹,等. 玉米生育期相关性状QTL定位[J]. 江苏农业科学,2022,50(8):63-68.
[13]郭爽,王栋,聂蕾,等. 玉米开花期相关性状的QTL定位与候选基因分析[J]. 种子,2023,42(6):1-8.
[14]李真,刘文童,杨硕,等. 玉米花期性状的全基因组关联分析[J]. 分子植物育种,2020,18(1):37-45.
[15]齐欣,黄洁,王延波,等. 玉米开花期相关性状的全基因组关联分析[J/OL]. 分子植物育种,2023:1-10(2023-03-01)[2024-01-02].http://kns.cnki.net/kcms/detail/46.1068.S.20230228.1659.023.html.
[16]杨慧丽,林亚楠,张怀胜,等. 玉米开花期性状的QTL及杂种优势位点定位[J]. 作物学报,2017,43(5):678-690.
[17]钱甫,张占琴,陈树宾,等. 基于GWAS和WGCNA分析挖掘玉米花期相关候选基因[J]. 作物学报,2023,49(12):3261-3276.
[18]Coles N D,McMullen M D,Balint-Kurti P J,et al. Genetic control of photoperiod sensitivity in maize revealed by joint multiple population analysis[J]. Genetics,2010,184(3):799-812.
[19]Ducrocq S,Madur D,Veyrieras J B,et al. Key impact of Vgt1 on flowering time adaptation in maize:evidence from association mapping and ecogeographical information[J]. Genetics,2008,178(4):2433-2437.
[20]Salvi S,Sponza G,Morgante M,et al. Conserved noncoding genomic sequences associated with a flowering-time quantitative trait locus in maize[J]. Proceedings of the National Academy of Sciences of the United States of America,2007,104(27):11376-11381.
[21]Meng X,Muszynski M G,Danilevskaya O N. The FT-like ZCN8 gene functions as a floral activator and is involved in photoperiod sensitivity in maize[J]. The Plant Cell,2011,23(3):942-960.
[22]李燕,陶柯宇,胡悦,等. 玉米ZCN7在调控花期抗旱性中的作用[J]. 中国农业科学,2023,56(16):3051-3061.
[23]Thornsberry J M,Goodman M M,Doebley J,et al. Dwarf8 polymorphisms associate with variation in flowering time[J]. Nature Genetics,2001,28(3):286-289.
[24]Huang C,Sun H Y,Xu D Y,et al. ZmCCT9 enhances maize adaptation to higher latitudes[J]. Proceedings of the National Academy of Sciences,2018,115(2):334-341.
[25]Colasanti J,Yuan Z,Sundaresan V. The indeterminate gene encodes a zinc finger protein and regulates a leaf-generated signal required for the transition to flowering in maize[J]. Cell,1998,93(4):593-603.
[26]Muszynski M G,Dam T,Li B L,et al. Delayed flowering1 encodes a basic leucine zipper protein that mediates floral inductive signals at the shoot apex in maize[J]. Plant Physiology,2006,142(4):1523-1536.
[27]Yan J B,Yang X H,Shah T,et al. High-throughput SNP genotyping with the goldengate assay in maize[J]. Molecular Breeding,2010,25(3):441-451.
[28]Meng L,Li H H,Zhang L Y,et al. QTL IciMapping:Integrated software for genetic linkage map construction and quantitative trait locus mapping in biparental populations[J]. The Crop Journal,2015,3(3):269-283.
[29]武博洋,彭云玲,赵小强,等. 玉米苗期在盐胁迫下耐盐相关QTL分析[J]. 分子植物育种,2019,17(1):154-160.
[30]McCouch S R,Cho Y G,Yano M,et al. Report on QTL nomenclature[J]. Rice Genetics Newsletter,1997,14:11-13.
[31]陈俊宇,卢峰,陈婉莹,等. 玉米生育期相关性状QTL定位[J]. 江苏农业科学,2022,50(8):63-68.
[32]张春宵,李淑芳,金峰学,等. 用3种方法定位玉米萌发期和苗期的耐盐和耐碱相关性状QTL[J]. 作物学报,2019,45(4):508-521.
[33]苏成付,赵团结,盖钧镒. 不同统计遗传模型QTL定位方法应用效果的模拟比较[J]. 作物学报,2010,36(7):1100-1107.
[34]石超男,张战辉,唐贵良,等. 基于RIL群体和IF2群体的玉米开花期相关性状QTL分析[J]. 河南农业大学学报,2016,50(2):147-156.
[35]Messmer R,Fracheboud Y,Bnziger M,et al. Drought stress and tropical maize:QTL-by-environment interactions and stability of QTLs across environments for yield components and secondary traits[J]. Theoretical and Applied Genetics,2009,119(5):913-930.
[36]曹浩飞,王彬,毛克举,等. 基于单片段代换系群体的玉米开花期性状QTL分析[J]. 河南农业大学学报,2014,48(1):6-10.
[37]Fu F L,Feng Z L,Gao S B,et al. Evaluation and quantitative inheritance of several drought-relative traits in maize[J]. Agricultural Sciences in China,2008,7(3):280-290.
[38]Wang L W,Zhou Z Q,Li R G,et al. Mapping QTL for flowering time-related traits under three plant densities in maize[J]. The Crop Journal,2021,9(2):372-379.

相似文献/References:

[1]孙建伟.水涝胁迫对玉米细胞保护酶同工酶的影响[J].江苏农业科学,2013,41(04):85.
[2]刘荣,张卫建,齐华,等.密植型玉米“中单909”高产群体结构特征[J].江苏农业科学,2013,41(05):56.
 Liu Rong,et al.Study on high yield population structure of close planting maize cultivar “Zhongdan 909”[J].Jiangsu Agricultural Sciences,2013,41(16):56.
[3]沈浜凯,肖龙云,冯乃杰,等.黄腐酸和AM真菌对玉米幼苗抗旱性的影响[J].江苏农业科学,2013,41(05):64.
 Shen Bangkai,et al.Effects of fulvic acid and AM fungi on drought resistance of maize seedlings[J].Jiangsu Agricultural Sciences,2013,41(16):64.
[4]张金然,缑艳霞,孙丽鹏.固氮螺菌157对玉米、向日葵的促生长作用[J].江苏农业科学,2014,42(12):116.
 Zhang Jinran,et al.Effects of Azospirillum 157 on growth of maize and sunflower[J].Jiangsu Agricultural Sciences,2014,42(16):116.
[5]白小军,吴燕,牛艳,等.玉米中乙草胺和莠去津残留量GC-MS/MS分析法的建立[J].江苏农业科学,2014,42(11):334.
 Bai Xiaojun,et al().Establishment of GC-MS/MS analysis method of acetochlor and atrazine residues in maize[J].Jiangsu Agricultural Sciences,2014,42(16):334.
[6]邹晓威,王娜,刘芬,等.玉米抗病相关基因在玉米与玉米丝黑穗病菌、玉米黑粉病菌互作过程中的表达差异分析[J].江苏农业科学,2014,42(11):150.
 Zou Xiaowei,et al(0).Different expression of resistance-related genes between Sporisorium reilianum and Ustilago maydis interact with corn[J].Jiangsu Agricultural Sciences,2014,42(16):150.
[7]杨洪兴,陈静,陈艳萍.江苏省玉米机械化生产的发展及育种对策思考[J].江苏农业科学,2014,42(11):116.
 Yang Hongxing,et al().Development and breeding strategy of mechanized production of maize in Jiangsu Province[J].Jiangsu Agricultural Sciences,2014,42(16):116.
[8]张丽妍,霍剑锋,孟繁盛,等.不同肥料、施肥水平及施用方法对玉米产量、性状及效益的影响[J].江苏农业科学,2014,42(11):119.
 Zhang Liyan,et al (9).Effects of different fertilizers,fertilizer levels and fertilizing methods on yield,characters and benefit of maize[J].Jiangsu Agricultural Sciences,2014,42(16):119.
[9]王雷,崔震海,张立军.玉米C4型PEPC全长基因的克隆与表达载体构建[J].江苏农业科学,2014,42(11):26.
 Wang Lei,et al().Cloning and expression vector construction of full-length C4 type PEPC gene in maize[J].Jiangsu Agricultural Sciences,2014,42(16):26.
[10]雷恩,赵光明,刘艳红.不同稀释浓度松土保水剂对玉米营养生长的影响[J].江苏农业科学,2013,41(06):77.
 Lei En,et al.Effect of different dilutions of super absorbent polymer on vegetative growth of maize[J].Jiangsu Agricultural Sciences,2013,41(16):77.

备注/Memo

备注/Memo:
收稿日期:2024-01-30
基金项目:广西创新驱动发展专项资金项目(编号:桂科AA22068095);广西农业科学院基本科研业务专项(编号:桂农科2021YT016);广西农业科学院基本科研业务专项(编号:桂农科2023YM57)。
作者简介:陈坤(1984—),男,硕士,助理研究员,主要从事玉米遗传育种研究。E-mail:kuncn@sina.com。
通信作者:卢生乔,副研究员,主要从事玉米遗传育种研究,E-mail:252673079@qq.com;张述宽,研究员,从事作物遗传育种研究,E-mail:790039187@qq.com。
更新日期/Last Update: 2024-08-20