[1]刘燕,曾维军,杨秀忠.威宁短柱油茶根围丛枝菌根真菌多样性调查[J].江苏农业科学,2024,52(17):268-276.
 Liu Yan,et al.Investigation on arbuscular mycorrhizal fungi diversity in rhizosphere of Camellia weiningensis[J].Jiangsu Agricultural Sciences,2024,52(17):268-276.
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威宁短柱油茶根围丛枝菌根真菌多样性调查()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第52卷
期数:
2024年第17期
页码:
268-276
栏目:
资源与环境
出版日期:
2024-09-05

文章信息/Info

Title:
Investigation on arbuscular mycorrhizal fungi diversity in rhizosphere of Camellia weiningensis
作者:
刘燕1曾维军1杨秀忠2
1.贵州省生物研究所,贵州贵阳 550009; 2.威宁自治县山地特色农业科学研究所,贵州威宁 553100
Author(s):
Liu Yanet al
关键词:
威宁短柱油茶丛枝菌根真菌高通量测序土壤因子多样性形态学鉴定
Keywords:
-
分类号:
S718.81;S794.4
DOI:
-
文献标志码:
A
摘要:
为了揭示高寒山区野生油茶(威宁短柱油茶)根围土壤丛枝菌根真菌(AMF)的结构与组成,解析土壤因子对AMF群落组成及其多样性的影响,为高寒山区抗逆性AMF资源的发掘及物种多样性研究提供理论依据,以贵州省高寒山区分布的威宁短柱油茶7个样地根围土壤为材料,采用高通量测序结合形态学鉴定的方法,研究威宁短柱油茶AMF的侵染情况、群落结构、遗传多样性、AMF种类,并进行土壤因子与AMF相关性分析。结果显示,威宁短柱油茶AMF侵染率在不同样地中存在差异。7个样地的AMF菌丝侵染率、丛枝侵染率、泡囊浸染率、总侵染率、侵染强度的平均值分别为59.52%、13.49%、51.59%、65.87%、61.11%。通过高通量测序,7个样地共鉴定到5属46种AMF,其中球囊霉属(Glomus)的相对丰度最高,为优势类群;VTX00223为优势虚拟种。7个样地的AMF群落组成和相对丰度差异较大,但ɑ多样性无显著差异。RDA分析表明,全磷、速效钾、碱解氮含量对无梗囊霉属(Acaulospora)的基因丰度有正向影响,盾巨孢囊霉属(Scutellospora)、类球囊霉属(Paraglomus)的基因丰度与全磷含量相关,而全钾含量影响球囊霉属的基因丰度。此外,通过形态学,鉴定到4属13种AMF;其中球囊霉属6种,无梗囊霉属5种,盾巨孢囊霉属、原囊霉属各1种;G.sp1出现频率最高,为7个样地共有。贵州高寒山区野生油茶根围土壤中的AMF资源丰富,土壤因子是影响AMF侵染的一个重要因素,AMF种类对土壤因子有一定选择性。
Abstract:
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备注/Memo

备注/Memo:
收稿日期:2024-07-20
基金项目:贵州省林业科研项目(编号:黔林科合〔2022〕05号);贵州科学院博士基金(编号:黔科院R字[2021]2号);贵州省科技计划(编号:黔科合支撑[2022]重点 017 号)。
作者简介:刘燕(1981—),女,贵州贵阳人,博士,副研究员,主要从事植物种苗繁育及菌根真菌研究。E-mail:472395057@qq.com。
更新日期/Last Update: 2024-09-05