|本期目录/Table of Contents|

[1]王丽丽,杨谦.接种枯草芽孢杆菌和丛枝菌根真菌促进红三叶修复石油污染土壤[J].江苏农业科学,2016,44(05):526-529.
 Wang Lili,et al.Effect of inoculation with plant growth-promoting rhizobacteria (PGPR) of bacillus subtilis and arbuscular mycorrhizal fungi of Glomus geosporum on phytoremediation of Trifolium pratense to petroleum contaminated soil[J].Jiangsu Agricultural Sciences,2016,44(05):526-529.
点击复制

接种枯草芽孢杆菌和丛枝菌根真菌促进红三叶
修复石油污染土壤
(PDF)
分享到:

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第44卷
期数:
2016年05期
页码:
526-529
栏目:
资源与环境
出版日期:
2016-05-25

文章信息/Info

Title:
Effect of inoculation with plant growth-promoting rhizobacteria (PGPR) of bacillus subtilis and arbuscular mycorrhizal fungi of Glomus geosporum on phytoremediation of Trifolium pratense to petroleum contaminated soil
作者:
王丽丽12 杨谦1
1.哈尔滨工业大学生命科学与技术学院,黑龙江哈尔滨;2.哈尔滨市第一中学生物组,黑龙江哈尔滨
Author(s):
Wang Liliet al
关键词:
枯草芽孢杆菌丛枝菌根真菌红三叶石油污染土壤修复
Keywords:
-
分类号:
X53
DOI:
-
文献标志码:
A
摘要:
在温室内通过盆栽试验的方法,比较对照组(CK)、单纯接种枯草芽孢杆菌(Bacillus subtilis,简称Bs)、单纯接种丛枝菌根真菌——地球囊霉(Glomus geosporum,简称Gg)、混合接种Gg+Bs的红三叶种植于石油污染的土壤中后,植株与土壤理化性状差异。实验结果表明,经过60 d修复后,与对照组相比,单纯或混合接种处理均能显著增加红三叶地上和地下干生物量(P<0.05),可溶性糖含量和脯氨酸含量均显著升高(P<0.05),叶片CAT、POD活性显著升高(P<0.05),丙二醛含量明显减少(P<0.05)。在各种接种处理中,红三叶根际与非根际土壤脱氢酶活性和土壤中总石油烃降解率均显著升高(P<0.05)。Bs和Gg在红三叶修复石油污染土壤和植物抗石油胁迫的能力方面有协同作用,接种Bs+Gg处理修复效果要优于单独接种Bs或Gg。
Abstract:
-

参考文献/References:

[1]耿春女,李培军,陈素华,等. 不同AM真菌对三叶草耐油性的影响[J]. 应用与环境生物学报,2002,8(6):648-652.
[2]Arshad M,Shaharoona B,Mahmood T. Inoculation with plant growth promoting rhizobacteria containing ACC-deaminase partially eliminates the effects of water stress on growth,yield and ripening of Pisum sativum L.[J]. Pedosphere,2008,18(5):611-620
[3]赵硕伟,沈嘉澍,沈标.复合菌群的构建及其对石油污染土壤修复的研究[J]. 农业环境科学学报,2011,30(8):1567-1572.
[4]Huang X D,El-Alawi Y,Gurska J,et al. A multi-process phytoremediation system for decontamination of persistent total petroleum hydrocarbons (TPHs) from soils[J]. Microchemical Journal,2005,81:139-147.
[5]Hong S H,Ryu H W,Kim J,et al. Rhizoremediation of diesel-contaminated soil using the plant growth-promoting rhizobacterium Gordonia sp. S2RP-17[J]. Biodegradation,2011,22:593-601.
[6]龙伟文,王平,冯新梅,等. PGPR与AMF相互关系的研究进展[J]. 应用生态学报,2000,11(2):311-314.
[7]秦芳玲,田中民. 同时接种解磷细菌与丛枝菌根真菌对低磷土壤红三叶草养分利用的影响[J]. 西北农林科技大学学报:自然科学版,2009,37(6):151-157.
[8]张瑞芹,卢致霖,陈洁雯,等. AM真菌影响三叶草根系抗氧化酶活性的系统效应[J]. 微生物学通报,2011,38(3):322-327.
[9]张颖,高景慧.镉胁迫对红三叶种子萌发及幼苗生理特性的影响[J]. 西北农业学报,2007,16(3):57-59.
[10]何玮,蒋安,王琳,等. PEG 干旱胁迫对红三叶抗性生理生化指标的影响研究[J]. 中国农学通报,2013,29(5):5-10.
[11]孔令慧,赵桂琴. 不同品种红三叶苗期对4 ℃低温胁迫的生理响应[J]. 中国草地学报,2013,35(3):31-37.
[12]吉云秀. 含ACC脱氨酶PGPR分离及提高植物抗逆性[D]. 大连:大连海事大学,2008.
[13]Phillips J M,Hayman D S.Improved procedures for clearingand staining parasitic and vesicular-arbuscular mycorrhizalfungi for rapid assessment of infection[J]. Trans Br Mycol Soc,1970,55:158-161.
[14]李玲. 植物生理学实验指导[M]. 北京:科学出版社,2009:10-11.
[15]刘光崧. 土壤理化分析与剖面描述[M]. 北京:中国标准出版社,1996.
[16]简令成,王红. 逆境植物细胞生物学[M]. 北京:科学出版社,2009.
[17]何洁,贺鑫.石油对翅碱蓬生长及生理特性的影响[J]. 农业环境科学学报 2011,30(4):650-655.
[18]岳冰冰,李鑫,任芳菲,等. 石油污染对紫花苜蓿部分生理指标的影响[J]. 草业科学,2011,28(2):236-240.
[19]李小利,刘国彬. 土壤石油污染对植物苗期生长和土壤呼吸的影响[J]. 水土保持学报,2007,21(3):95-98.
[20]张金林,李惠茹,郭姝媛,等. 高等植物适应盐逆境研究进展[J]. 草业学报,2015,24(12):220-236.
[21]Linderman R G. Role of VAM fungi in biocontrol[M]//Pfleger F L,Linderman R G. Mycorrhizae and plant health.St Paul,MN:APS Press,1994:1-26.
[22]Kohler J,Hernández J A,Caravaca F,et al. Induction of antioxidant enzymes is involved in the greater effectiveness of a PGPR versus AM fungi with respect to increasing the tolerance of lettuce to severe salt stress[J]. Environmental and Experimental Botany,2009,65:245-252.
[23]Kohler J,Caravaca F,Carrasco L,et al. Interactions between a plant growth-promoting rhizobacterium,an AM fungus and a phosphate-solubilising fungus in the rhizosphere of Lactuca sativa[J]. Applied Soil Ecology,2007,35:480-487.
[24]耿春女,李培军,陈素华,等. 不同丛枝菌根真菌对万寿菊生长及柴油降解率的影响[J]. 应用生态学报,2003,14(10):1775-1779.

相似文献/References:

[1]郭凤柳,张海颖,李勇,等.马铃薯疮痂病拮抗菌株B1的鉴定及防效测定[J].江苏农业科学,2013,41(05):90.
 Guo Fengliu,et al.Identification and control efficiency of biocontrol strain B1 for potato scab pathogens[J].Jiangsu Agricultural Sciences,2013,41(05):90.
[2]夏彦飞,郑伟,苏盼盼,等.枯草芽孢杆菌OKB105菌株不同培养基发酵上清液的杀线活性[J].江苏农业科学,2014,42(12):144.
 Xia Yanfei,et al.Nematicidal activities of different fermentation cultured supernatants of Bacillus subtilis strain OKB105 against plant-parasitic nematodes[J].Jiangsu Agricultural Sciences,2014,42(05):144.
[3]刘琴,王艳,徐健,等.枯草芽孢杆菌Bs-1013的抑菌活性及其包衣对黄瓜发芽生长的影响[J].江苏农业科学,2013,41(11):117.
 Liu Qin,et al.Antibiotic activity of Bacillus subtilis Bs-1013 and effect of its coating agent on seed germination and growth of cucumber[J].Jiangsu Agricultural Sciences,2013,41(05):117.
[4]李少朋,毕银丽,彭星.接种丛枝菌根真菌对矿井水回灌玉米生长的影响[J].江苏农业科学,2016,44(05):112.
 Li Shaopeng,et al.Effects of inoculation with AMF on growth of maize after recharging with mining water[J].Jiangsu Agricultural Sciences,2016,44(05):112.
[5]伍善东,刘冬华,郭照辉,等.1株高效解无机磷细菌JP-7的分离、鉴定及溶磷能力分析[J].江苏农业科学,2015,43(12):374.
 Wu Shandong,et al.Isolation and identification of a high efficiency inorganic phosphorus-dissolving bacteria from soil and its analysis of phosphate solubilization[J].Jiangsu Agricultural Sciences,2015,43(05):374.
[6]张婷婷,马磊.蜡样芽孢杆菌启动子片段克隆及转录活性分析[J].江苏农业科学,2014,42(04):25.
 Zhang Tingting,et al.Cloning and transcription of promoter fragment in Bacillus cereus[J].Jiangsu Agricultural Sciences,2014,42(05):25.
[7]关一鸣,潘晓曦,王莹,等.哈茨木霉菌、枯草芽孢杆菌对人参灰霉病和根腐病病原菌的拮抗作用[J].江苏农业科学,2014,42(05):123.
 Guan Yiming,et al.Antagonism of Trichoderma harzianum and Bacillus subtilis against Botrytis cinerea Pers. and Fusarium solani on ginseng[J].Jiangsu Agricultural Sciences,2014,42(05):123.
[8]林志伟,肖亚静,郭春兰,等.枯草芽孢杆菌21代谢物对大豆茄镰孢菌的抑菌机制[J].江苏农业科学,2015,43(11):175.
 Lin Zhi Wei,et al.Primary study on restrain mechanism of Bacillus subtilis strain 21 metabolite to soybean Fusarium solani[J].Jiangsu Agricultural Sciences,2015,43(05):175.
[9]范瑛阁,李 莎,赵 静,等.枯草芽孢杆菌H1和H2对黄瓜的促生作用[J].江苏农业科学,2015,43(07):158.
 Fan Yinge,et al.Promoting effect of Bacillus subtilis H1 and H2 on cucumber[J].Jiangsu Agricultural Sciences,2015,43(05):158.
[10]谢鹏,付胜勇,常玲玲,等.枯草芽孢杆菌对乳鸽生长性能、小肠形态和结直肠菌群的影响[J].江苏农业科学,2015,43(06):190.
 Xie Peng,et al.Effects of Bacillus subtilis on growth performance,intestinal morphology and colorectal microbiota of pigeon squabs[J].Jiangsu Agricultural Sciences,2015,43(05):190.

备注/Memo

备注/Memo:
收稿日期:2016-01-20
基金项目:国家“863”计划(编号:2012AA021404)。
作者简介:王丽丽(1980—),女,黑龙江哈尔滨人,博士研究生,主要从事环境微生物方面的研究。E-mail:wanglilihit@126.com。
通信作者:杨谦,教授,博士生导师,主要从事环境微生物方面的研究。E-mail:yangq@hit.edu.cn。
更新日期/Last Update: 2016-05-25