|本期目录/Table of Contents|

[1]丁夏威,田沺,陈健文,等.甘薯WRKY基因家族鉴定及其响应茎腐病侵染的表达分析[J].江苏农业科学,2024,52(21):25-39.
　Ding Xiawei,et al.Identification of WRKY gene family and their expression analysis in response to stem rot infection in Ipomoea batatas[J].Jiangsu Agricultural Sciences,2024,52(21):25-39.
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甘薯WRKY基因家族鉴定及其响应茎腐病侵染的表达分析(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第52卷
期数:
2024年第21期
页码:
25-39
栏目:
生物技术
出版日期:
2024-11-05

文章信息/Info

Title:
Identification of WRKY gene family and their expression analysis in response to stem rot infection in Ipomoea batatas
作者:
丁夏威1; 2; 田沺2; 3; 陈健文1; 陈景益2; 王章英2; 邹宏达2; 黄立飞2
1.华南农业大学农学院，广东广州510640； 2.广东省农业科学院作物研究所/广东省农作物遗传改良重点实验室，广东广州 510640； 3.仲恺农业工程学院农学院，广东广州 510640
Author(s):
Ding Xiawei; et al
关键词:
甘薯; WRKY; 茎腐病; 生物信息学; 表达模式
Keywords:
-
分类号:
S531.03；S435.311
DOI:
-
文献标志码:
A
摘要:
WRKY基因家族是植物中重要的转录因子家族，在植物响应生物和非生物胁迫过程中起到非常重要的调控作用。通过生物信息学方法在甘薯基因组(pasi3)鉴定了84个WRKY家族基因，分布于15条染色体，编码氨基酸数量为120~838个，等电点为4.89~10.74。系统发育树将其分为Ⅰ、Ⅱ、Ⅲ簇，其中Ⅱ簇又分为5个亚群(Ⅱ-a、Ⅱ-b、Ⅱ-c、Ⅱ-d、Ⅱ-e)。保守结构域显示，IbWRKY基因具有高度保守的WRKYGQK基序，其中有11个基因的保守基序发生了变异。IbWRKY基因的启动子中存在多个与生长发育、激素响应和应激反应相关的顺式作用元件。共线性分析结果表明，甘薯与拟南芥、番茄和马铃薯等双子叶植物的WRKY基因具有相似的进化历程。IbWRKY基因在甘薯中具有显著的组织特异性，且在生物、非生物胁迫和激素处理下表达差异显著。73个IbWRKY基因参与了茎腐病胁迫响应，其中IbWRKY20/40/6/84等4个基因与茎腐病抗性密切相关。本研究鉴定了84个甘薯WRKY基因，并明确了其家族特征、进化和表达模式。同时，挖掘到了4个与茎腐病抗性密切相关的WRKY基因。
Abstract:
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备注/Memo

备注/Memo:
收稿日期：2023-10-13
基金项目：国家甘薯产业技术体系建设专项(编号：CARS-10)；广东省科技计划(编号：2023B0202010019)。
作者简介：丁夏威(1998—)，女，河南周口人，硕士，从事甘薯抗病遗传育种。E-mail：dingxiawei0224@163.com。
通信作者：黄立飞，博士，研究员，从事甘薯抗病遗传育种研究，E-mail：hlf157@163.com；邹宏达，博士，副研究员，从事甘薯遗传育种，E-mail：zouhongda@gdaas.cn。
更新日期/Last Update: 2024-11-05