|本期目录/Table of Contents|

[1]凤舞剑,张莹,韩波,等.基于转录组和AlphaFold快速鉴定水稻特异性响应稻瘟病菌侵染的转录因子和靶标[J].江苏农业科学,2025,53(4):23-30.
　Feng Wujian,et al.Rapid identification of rice-specific transcription factors and targets in response to Magnaporthe oryzae infection based on transcriptome and AlphaFold[J].Jiangsu Agricultural Sciences,2025,53(4):23-30.
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基于转录组和AlphaFold快速鉴定水稻特异性响应稻瘟病菌侵染的转录因子和靶标(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第53卷
期数:
2025年第4期
页码:
23-30
栏目:
抗病基因
出版日期:
2025-02-20

文章信息/Info

Title:
Rapid identification of rice-specific transcription factors and targets in response to Magnaporthe oryzae infection based on transcriptome and AlphaFold
作者:
凤舞剑; 张莹;  韩波; 强承魁
徐州生物工程职业技术学院/徐州市现代农业生物技术重点实验室，江苏徐州 221006
Author(s):
Feng Wujian; et al
关键词:
转录因子; 稻瘟病菌; 水稻; AlphaFold3; 作用靶标
Keywords:
-
分类号:
S435.111.4+1
DOI:
-
文献标志码:
A
摘要:
转录因子是调控植物免疫响应的关键因子之一。为鉴定水稻早期响应稻瘟病菌侵染的关键转录因子及相互作用的靶基因，探究早期病原菌诱导型转录因子的调控功能，为水稻的免疫机制研究和抗病种质创制提供理论依据，利用生物信息学方法对水稻基因组中的转录因子进行鉴定，通过水稻-稻瘟病菌互作转录组和Mfuzz表达模式特征分析，筛选受稻瘟病菌诱导的转录因子和参与调控的功能。利用AlphaFold3对关键的转录因子和靶基因构建蛋白-DNA相互作用的复合体模型，并对转录因子潜在的结合靶标进行筛选。结果在水稻中总共鉴定到1 948个转录因子，约89类转录因子。其中1、3、2、6号染色体的转录因子数目较多，分别为279、250、235、176个。共鉴定到673个转录因子受稻瘟病菌侵染水稻诱导，其中主要包含11%的WRKY、11%的MYB和11%的C2H2转录因子。这些转录因子主要调控植物信号转导、植物-病原菌的相互作用和MAPK信号、响应乙烯、响应水杨酸、响应茉莉酸、天然免疫和对细菌的防御响应等功能；鉴定到集合1和集合6中的转录因子受病原菌特异性诱导后表达量持续增加；利用AlphaFold3发现Os5t0343400编码的WRKY转录因子与SIB1基因启动子存在互作。结果表明，水稻在响应稻瘟病菌早期侵染中，约172个转录因子通过调控激素和免疫防御等途径响应稻瘟病菌的侵染，并预测到1个响应病原菌的转录因子及作用靶标在水稻免疫响应中发挥重要作用。
Abstract:
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备注/Memo

备注/Memo:
收稿日期：2024-11-04
基金项目：现代农业产业科技服务项目(编号：KC23139)；江苏省徐州市科技重点研发项目(编号：KC21121)。
作者简介：凤舞剑(1976—)，男，江苏睢宁人，硕士，副教授，主要从事农业有害生物菌原分离、鉴定及综合防控技术研究。E-mail：fwjedu@126.com。
更新日期/Last Update: 2025-02-20