[1]刘洁,李娟娟,马香,等.微塑料对中国土壤微生物群落结构影响的综合分析[J].江苏农业科学,2025,53(15):255-261.
　Liu Jie,et al.Comprehensive analysis of impact of microplastics on soil microbial community structure in China[J].Jiangsu Agricultural Sciences,2025,53(15):255-261.
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微塑料对中国土壤微生物群落结构影响的综合分析()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第53卷
期数:
2025年第15期
页码:
255-261
栏目:
资源与环境
出版日期:
2025-08-05

文章信息/Info

Title:
Comprehensive analysis of impact of microplastics on soil microbial community structure in China
作者:
刘洁1; 2; 李娟娟1; 2; 马香1; 2; 迟雪1; 2; 唐燕琼1; 2; 刘柱1; 2; 李宏1; 2
1.海南大学生命健康学院，海南海口 570228； 2.海南大学海南省全健康研究重点实验室，海南海口 570228
Author(s):
Liu Jie; et al
关键词:
微塑料; 土壤; 微生物群落; 机器学习模型; 细菌分类; LDA; 芽孢杆菌属
Keywords:
-
分类号:
S154.3
DOI:
-
文献标志码:
A
摘要:
微生物是土壤生态系统的关键组成部分，微塑料的累积可能极大地扰乱了土壤中的微生物组成，进而对土壤的自然功能和生态系统的稳定性产生持久的负面效应。综合分析了来自我国的210个土壤样本，深入探讨了微塑料对土壤微生物群落结构的影响。研究显示，无论是可生物降解还是不可生物降解的微塑料，被引入土壤后均明显增加了样本中细菌群落的多样性和分布均匀性。特别是在细菌分类的门水平上，诸如放线菌门、变形菌门和厚壁菌门等主要菌群的相对比例出现了明显的变动。属水平上，盖勒氏菌属和芽孢杆菌属的相对丰度在微塑料处理的土壤样本中明显高于对照组。LDA分析进一步揭示了对土壤细菌群落分类具有显著影响的前20个关键微生物属。在健康土壤中，盖勒氏菌属等5个细菌属占主要地位，代表了土壤的健康基线。可生物降解微塑料促进了慢生根瘤菌属等12个属的富集，而不可生物降解微塑料则使土壤红杆菌属等3个属显著增加。通过构建的可解释机器学习模型，芽孢杆菌属被筛选为土壤微塑料降解的潜在生物标志物。本研究结果不仅揭示了微塑料对土壤微生物群落结构的影响，还为土壤环境的改善提供了参考依据。
Abstract:
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参考文献/References:

［1］Wang T，Hu M H，Xu G G，et al. Microplastic accumulation via trophic transfer：can a predatory crab counter the adverse effects of microplastics by body defence？［J］. Science of the Total Environment，2021，754：142099.
［2］Xu H W，Qu Q，Chen Y H，et al. Responses of soil enzyme activity and soil organic carbon stability over time after cropland abandonment in different vegetation zones of the Loess Plateau of China［J］. CATENA，2021，196：104812.
［3］Torres F G，Dioses-Salinas D C，Pizarro-Ortega C I，et al. Sorption of chemical contaminants on degradable and non-degradable microplastics：Recent progress and research trends［J］. Science of the Total Environment，2021，757：143875.
［4］Wei W，Chen X M，Peng L，et al. The entering of polyethylene terephthalate microplastics into biological wastewater treatment system affects aerobic sludge digestion differently from their direct entering into sludge treatment system［J］. Water Research，2021，190：116731.
［5］Hou L Y，Kumar D，Yoo C G，et al. Conversion and removal strategies for microplastics in wastewater treatment plants and landfills［J］. Chemical Engineering Journal，2021，406：126715.
［6］Nizzetto L，Futter M，Langaas S. Are agricultural soils dumps for microplastics of urban origin？［J］. Environmental Science & Technology，2016，50(20)：10777-10779.
［7］Okoroafor T，Haile S，Velasquez-Orta S. Life cycle assessment of microbial electrosynthesis for commercial product generation［J］. Journal of Hazardous，Toxic，and Radioactive Waste，2021，25(1)：04020062.
［8］Deng H，He J X，Feng D，et al. Microplastics pollution in mangrove ecosystems：a critical review of current knowledge and future directions［J］. Science of the Total Environment，2021，753：142041.
［9］钱亚茹，石磊磊，沈茜，等. 淡水环境中微塑料污染及毒性效应研究进展［J］. 环境工程技术学报，2022，12(4)：1096-1104.
［10］Wang S X，Hu M H，Zheng J H，et al. Ingestion of nano/micro plastic particles by the mussel Mytilus coruscus is size dependent［J］. Chemosphere，2021，263：127957.
［11］王志超，李哲，李嘉辰，等. 聚乙烯微塑料对盐渍化土壤微生物群落的影响［J］. 环境科学，2024，45(5)：3088-3097.
［12］Fei Y F，Huang S Y，Zhang H B，et al. Response of soil enzyme activities and bacterial communities to the accumulation of microplastics in an acid cropped soil［J］. Science of the Total Environment，2020，707：135634.
［13］李成涛，吴婉晴，梁右才，等. 含有苯环结构的PBAT微塑料生物降解对土壤微生物群落结构的影响［J］. 陕西科技大学学报，2023，41(2)：22-30.
［14］Lewis S，Nash A，Li Q H，et al. Comparison of 16S and whole genome dog microbiomes using machine learning［J］. BioData Mining，2021，14(1)：41.
［15］Jasner Y，Belogolovski A，Ben-Itzhak M，et al. Microbiome preprocessing machine learning pipeline［J］. Frontiers in Immunology，2021，12：677870.
［16］Liu Y T，Zhang P，Sheng H M，et al. 16S rRNA gene sequencing and machine learning reveal correlation between drug abuse and human host gut microbiota［J］. Addiction Biology，2023，28(10)：e13311.
［17］Peng S Y，Luo M，Long D B，et al. Full-length 16S rRNA gene sequencing and machine learning reveal the bacterial composition of inhalable particles from two different breeding stages in a piggery［J］. Ecotoxicology and Environmental Safety，2023，253：114712
［18］Machado A A D，Lau C W，Kloas W，et al. Microplastics can change soil properties and affect plant performance［J］. Environmental Science & Technology，2019，53(10)：6044-6052.
［19］Cao J，Wang Q，Lei Y M，et al. Accumulation of microplastics and TCEP pollutants in agricultural soil：exploring the links between metabolites and gut microbiota in earthworm homeostasis［J］. Environment International，2022，170：107590.
［20］Zhang P，Wang J，Huang L，et al. Microplastic transport during desertification in drylands：abundance and characterization of soil microplastics in the amu darya-Aral sea basin，central Asia［J］. Journal of Environmental Management，2023，348：119353.
［21］Rong L L，Zhao L F，Zhao L C，et al. LDPE microplastics affect soil microbial communities and nitrogen cycling［J］. Science of the Total Environment，2021，773：145640.
［22］Zhang S W，Pei L，Zhao Y X，et al. Effects of microplastics and nitrogen deposition on soil multifunctionality，particularly C and N cycling［J］. Journal of Hazardous Materials，2023，451：131152.
［23］Puglisi E，Romaniello F，Galletti S，et al. Selective bacterial colonization processes on polyethylene waste samples in an abandoned landfill site［J］. Scientific Reports，2019，9(1)：14138.
［24］Li X N，Yao S，Bian Y R，et al. The combination of biochar and plant roots improves soil bacterial adaptation to PAH stress：Insights from soil enzymes，microbiome，and metabolome［J］. Journal of Hazardous Materials，2020，400：123227.
［25］Zhou Y J，Zhou S L. Role of microplastics in microbial community structure and functions in urban soils［J］. Journal of Hazardous Materials，2023，459：132141.
［26］Liao Y L，Tang Q X，Yang J Y. Microplastic characteristics and microplastic-heavy metal synergistic contamination in agricultural soil under different cultivation modes in Chengdu，China［J］. Journal of Hazardous Materials，2023，459：132270.
［27］Long B B，Li F Y，Wang K，et al. Impact of plastic film mulching on microplastic in farmland soils in Guangdong province，China［J］. Heliyon，2023，9(6)：e16587.
［28］Wang Y J，Liu L，Cao S，et al. Spatio-temporal variation of soil microplastics as emerging pollutant after long-term application of plastic mulching and organic compost in apple orchards［J］. Environmental Pollution，2023，328：121571.
［29］Li N Y，Qu J H，Yang J Y. Microplastics distribution and microbial community characteristics of farmland soil under different mulch methods［J］. Journal of Hazardous Materials，2023，445：130408.
［30］Li W L，Xiao Y. Microplastics increase soil microbial network complexity and trigger diversity-driven community assembly［J］. Environmental Pollution，2023，333：122095.
［31］Shen H R，Sun Y H，Duan H B，et al. Effect of PVC microplastics on soil microbial community and nitrogen availability under laboratory-controlled and field-relevant temperatures［J］. Applied Soil Ecology，2023，184：104794.
［32］Yang L Y，Liang H Y，Wu Q，et al. Biochar alleviated the toxic effects of microplastics-contaminated geocarposphere soil on peanut (Arachis hypogaea L.) pod development：roles of pod nutrient metabolism and geocarposphere microbial modulation［J］. Journal of the Science of Food and Agriculture，2024，104(5)：2990-3001.
［33］Iqbal B，Javed Q，Khan I，et al. Influence of soil microplastic contamination and cadmium toxicity on the growth，physiology，and root growth traits of Triticum aestivum L.［J］. South African Journal of Botany，2023，160：369-375.
［34］Sun B，Bai Z H，Bao L J，et al. Bacillus subtilis biofertilizer mitigating agricultural ammonia emission and shifting soil nitrogen cycling microbiomes［J］. Environment International，2020，144：105989.
［35］Hui C，Wei R，Jiang H，et al. Characterization of the ammonification，the relevant protease production and activity in a high-efficiency ammonifier Bacillus amyloliquefaciens DT［J］. International Biodeterioration & Biodegradation，2019，142：11-17.
［36］Wang J J，Zhao S Q，Xu S，et al. Co-inoculation of antagonistic Bacillus velezensis FH-1 and Brevundimonas diminuta NYM3 promotes rice growth by regulating the structure and nitrification function of rhizosphere microbiome［J］. Frontiers in Microbiology，2023，14：1101773.
［37］Hossain S，Shukri Z N A，Waiho K，et al. Biodegradation of polyethylene (PE)，polypropylene (PP)，and polystyrene (PS) microplastics by floc-forming bacteria，Bacillus cereus strain SHBF2，isolated from a commercial aquafarm［J］. Environmental Science and Pollution Research International，2024，31(22)：32225-32245.
［38］Kim B，Lee S W，Jung E M，et al. Biosorption of sub-micron-sized polystyrene microplastics using bacterial biofilms［J］. Journal of Hazardous Materials，2023，458：131858.

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备注/Memo

备注/Memo:
收稿日期：2024-08-09
基金项目：海南省自然科学基金高层次人才项目(编号：322RC589)；国家自然科学基金地区科学基金(编号：32060153)。
作者简介：刘洁(1999—)，男，安徽阜阳人，硕士研究生，主要从事微塑料相关的生物信息学研究。E-mail：19942452067@163.com。
通信作者：李宏，博士，副教授，主要从事生物信息学研究。E-mail：lihongbio@hainanu.edu.cn。
更新日期/Last Update: 2025-08-05