|本期目录/Table of Contents|

[1]丁锦平.棉花病毒诱导基因沉默体系构建[J].江苏农业科学,2013,41(06):38-40.
 Ding Jinping.Establishment of virus induced gene silencing system in cotton[J].Jiangsu Agricultural Sciences,2013,41(06):38-40.
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棉花病毒诱导基因沉默体系构建(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第41卷
期数:
2013年06期
页码:
38-40
栏目:
生物技术
出版日期:
2013-06-25

文章信息/Info

Title:
Establishment of virus induced gene silencing system in cotton
作者:
丁锦平
商丘师范学院生命科学学院/商丘师范学院植物与微生物互作重点实验室,河南商丘 476000
Author(s):
Ding Jinping
关键词:
棉花基因沉默病毒载体TRVPDS
Keywords:
-
分类号:
Q786
DOI:
-
文献标志码:
A
摘要:
探索了病毒诱导基因沉默载体在棉花上的应用,由烟草脆裂病毒(TRV)改造的pTV00载体,构建了棉花标记PDS基因病毒诱导基因沉默的载体,成功建立了棉花病毒诱导基因沉默体系。
Abstract:
-

参考文献/References:

[1]陶小荣,周雪平,崔晓峰,等. 病毒诱导的基因沉默及其在植物基因功能研究中的应用[J]. 生物化学与生物物理进展,2004,31(9):777-783.
[2]Burch-Smith T M,Anderson J C,Martin G B,et al. Applications and advantages of virus-induced gene silencing for gene function studies in plants[J]. The Plant Journal,2004,39(5):734-746.
[3]Kumagai M H,Donson J,Della-Cioppa G,et al. Cytoplasmic inhibition of carotenoid biosynthesis with virus-derived RNA[J]. Proc Natl Acad Sci USA,1995,92(5):1679-1683.
[4]Ratcliff F,Martin-Hernandez A M,Baulcombe D C. Tobacco rattle virus as a vector for analysis of gene function by silencing[J]. The Plant Journal,2001,25(2):237-245.
[5]Turnage M A,Muangsan N,Peele C G,et al. Germinivirus-based vectors for gene silencing in Arabidopsis[J]. The Plant Journal,2002,30(1):107-114.
[6]Holzberg S,Brosio P,Gross C,et al. Barley stripe mosaic virus-induced gene silencing in a monocot plant[J]. The Plant Journal,2002,30(3):315-327.
[7]John R T,Idris A M,Judith K B,et al. Geminivirus-mediated gene silencing from cotton leaf crumple virus is enhanced by low temperature in cotton[J]. Plant Physiology,2008,148:41-50.
[8]张增艳,姚乌兰,辛志勇. 植物基因功能鉴定新工具——病毒诱导基因沉默技术的研究进展[J]. 植物遗传资源学报,2006,7(1):100-105.

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备注/Memo

备注/Memo:
收稿日期:2013-01-01
基金项目:河南省科技攻关(编号:122102110213);河南省软科学研究(编号:122400410015)。
作者简介:丁锦平(1976—),女,河南长垣人,博士,讲师,研究方向为棉花种质创新及分子生物学。E-mail:jinpingding@163.com。
更新日期/Last Update: 2013-06-25