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[1]金素钰,王国生,徐亚欧,等.牦牛葡萄糖转运蛋白和单羧酸转运蛋白基因的克隆测序及在肌肉中的表达[J].江苏农业科学,2014,42(08):29-31.
　Jin Suyu,et al.Cloning， sequencing and expression in muscle of glucose transporter protein and single acid transporter protein genes in yak[J].Jiangsu Agricultural Sciences,2014,42(08):29-31.
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牦牛葡萄糖转运蛋白和单羧酸转运蛋白基因的克隆测序及在肌肉中的表达(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第42卷
期数:
2014年08期
页码:
29-31
栏目:
生物技术
出版日期:
2014-08-25

文章信息/Info

Title:
Cloning， sequencing and expression in muscle of glucose transporter protein and single acid transporter protein genes in yak
作者:
金素钰;  王国生;  徐亚欧;  林亚秋;  郑玉才
西南民族大学生命科学与技术学院，四川成都 610041
Author(s):
Jin Suyu; et al
关键词:
九龙牦牛; 葡萄糖转运蛋白; 单羧酸转运蛋白; 肌肉; 低氧适应; 基因表达
Keywords:
-
分类号:
S823.8+52
DOI:
-
文献标志码:
A
摘要:
采用RT-PCR方法克隆九龙牦牛(Bos grunniens)肌肉中葡萄糖转运蛋白(GLUT)和单羧酸转运蛋白(MCT)基因GlUT4、MCT1和MCT4的cDNA序列，并与普通牛(Bos taraus)进行基因序列和肌肉中mRNA水平的比较，以探索牦牛适应高原低氧的分子基础。结果表明，GlUT4、MCT1和MCT4的核苷酸序列和推导的氨基酸序列与普通牛的相似性均在99.5%以上。实时荧光定量PCR分析显示，成年九龙牦牛与黄牛背最长肌中GLUT4、MCT4基因 mRNA 水平无差别，而牦牛PMCT1基因mRNA水平显著低于黄牛(P<0.05)。乳酸脱氢酶(LDH)总活力和同工酶谱测定结果表明，牦牛背最长肌中LDH总活力、LDH5比例显著高于黄牛，显示出更强的无氧酵解能力。结合MCT1基因mRNA水平的差异发现，牦牛背最长肌对乳酸的氧化代谢能力低于黄牛，更倾向于无氧酵解，与其适应高原低氧有关。
Abstract:
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参考文献/References:

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备注/Memo

备注/Memo:
收稿日期：2013-11-07
基金项目：国家“973”计划前期研究专项(编号：2007CB116204)。
作者简介：金素钰(1965—)，女，重庆人，高级实验师，主要从事生物技术研究。
通信作者：郑玉才，博士，教授，主要从事高原动物分子遗传研究。E-mail：yucaizheng65@hotmail.com。
更新日期/Last Update: 2014-08-25