|本期目录/Table of Contents|

[1]张瑞,雍晓雨,周俊,等.分子生物学技术在产甲烷古菌多样性研究中的应用[J].江苏农业科学,2015,43(01):16-20.
　Zhang Rui,et al.Application of molecular biology technology in diversity research of methanogenic archaea[J].Jiangsu Agricultural Sciences,2015,43(01):16-20.
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分子生物学技术在产甲烷古菌多样性研究中的应用(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第43卷
期数:
2015年01期
页码:
16-20
栏目:
专论
出版日期:
2015-01-25

文章信息/Info

Title:
Application of molecular biology technology in diversity research of methanogenic archaea
作者:
张瑞1;  雍晓雨1; 2;  周俊1; 2;  吴美容1;  王舒雅1; 2;  闫志英3;  陈怡露1;  郑涛1; 2
1.南京工业大学生物与制药工程学院，江苏南京 211816； 2.南京工业大学生物能源研究所，江苏南京 211816；
3.中国科学院成都生物研究所，四川成都 610041
Author(s):
Zhang Rui; et al
关键词:
产甲烷古菌; 生物多样性; 分子生物学技术; 检测; 复合应用
Keywords:
-
分类号:
Q939.97
DOI:
-
文献标志码:
A
摘要:
产甲烷菌在自然界中分布广泛，是一类重要的严格厌氧原核微生物，其参与的产甲烷作用通常发生在厌氧发酵过程的最后一步，可将无机物或有机化合物最终转化成甲烷和二氧化碳。由于产甲烷菌独特的厌氧代谢机制，使其在自然界碳素循环过程中起着重要作用，因此对于产甲烷菌的多样性以及代谢机制的研究越来越受到人们的关注。相对于传统的培养检测方法，分子生物学技术对于产甲烷菌的多样性及其群落结构的检测更为便捷、准确和科学。介绍了产甲烷古菌的系统分类学发展，系统地阐述了产甲烷菌定性和定量的分子生物学检测方法，总结了不同技术在产甲烷菌多样性研究中的最新成果，最后提出多种技术的复合应用将成为研究的热点。
Abstract:
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备注/Memo

备注/Memo:
收稿日期：2014-03-07
基金项目：国家“973”计划(编号：2013CB733502)；国家自然科学基金(编号：21307058、21207065)；中国科学院环境与应用微生物重点实验室开放基金(编号：KLCAS-2013-05)；江苏省农业自主创新资金［编号：CX(13)3045］；江苏省高校自然科学研究面上项目(编号：13KJB610006)。
作者简介：张瑞(1990—)，女，江苏连云港人，硕士，主要从事微生物和分子生物学的研究。E-mail：zhangrui1030@njtech.edu.cn。
通信作者：郑涛，男，教授，主要从事生物能源、生物材料方向的研究。Tel：(025)58139929；E-mail：zhengtao@njtech.edu.cn。
更新日期/Last Update: 2015-01-25