|本期目录/Table of Contents|

[1]李莉萍,王瑞,梁万文,等.应用免疫冷冻超薄切片技术观察罗非鱼肠道M样细胞[J].江苏农业科学,2015,43(09):264-266.
 Li Liping,et al.Study on intestinal microfold cells of tilapia by ultra-thin cryosectioning and immunolabeling techniques[J].Jiangsu Agricultural Sciences,2015,43(09):264-266.
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应用免疫冷冻超薄切片技术观察罗非鱼肠道M样细胞(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第43卷
期数:
2015年09期
页码:
264-266
栏目:
水产养殖·特种种养
出版日期:
2015-09-25

文章信息/Info

Title:
Study on intestinal microfold cells of tilapia by ultra-thin cryosectioning and immunolabeling techniques
作者:
李莉萍1 王瑞1 梁万文1 黄婷1 甘西1 罗永巨1 李健2 石云良2 陈明1
1.广西水产科学研究院广西水产遗传育种与健康养殖重点实验室,广西南宁 530021; 2.广西大学动物科学技术学院,广西南宁 530005
Author(s):
Li Lipinget al
关键词:
罗非鱼M细胞荆豆凝集素
Keywords:
-
分类号:
S917;S942.5
DOI:
-
文献标志码:
A
摘要:
为鉴定罗非鱼肠道M样细胞及各肠道分布情况,利用FITC标记的荆豆凝集素(UEA-1)对罗非鱼前、中、后肠冰冻切片进行直接免疫荧光显微观察。结果发现,在罗非鱼前、中、后肠均观察到M样细胞;中肠和后肠M样细胞数量比前肠明显多。免疫组M样细胞数量显著比空白对照组多,且免疫组前肠数量明显多于空白对照组前肠。研究表明,在罗非鱼前、中、后肠存在M样细胞,肠道接触无乳链球菌弱毒抗原之后,在数量、分布上均发生变化,初步表明M样细胞参与肠道免疫应答。
Abstract:
-

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备注/Memo

备注/Memo:
收稿日期:2014-09-17
基金项目:广西科技发展“十二五”规划重大专项课题(编号:桂科重14121004-2-4);广西水产遗传育种与健康养殖重点实验室资助项目(编号:14-A-03-02);广西壮族自治区“八桂学者”岗位专项资金(编号:BGXZ-LFY-04);广西水产畜牧兽医局科技计划(编号:桂渔牧科1304527);广西水产畜牧科技推广应用项目(编号:桂渔牧科201452011)。
作者简介:李莉萍(1979—),女,广西南宁人,硕士,副研究员,主要从事水产疫苗与免疫技术研
更新日期/Last Update: 2015-09-25