|本期目录/Table of Contents|

[1]段明.谷子EPSPS基因的分离、修饰及表达载体的构建[J].江苏农业科学,2016,44(05):51-55.
　Duan Ming.Separation， modification and construction of expression vector for millet EPSPS gene[J].Jiangsu Agricultural Sciences,2016,44(05):51-55.
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谷子EPSPS基因的分离、修饰及表达载体的构建(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第44卷
期数:
2016年05期
页码:
51-55
栏目:
生物技术
出版日期:
2016-05-25

文章信息/Info

Title:
Separation， modification and construction of expression vector for millet EPSPS gene
作者:
段明
山西农业大学实验教学中心，山西太谷 030801
Author(s):
Duan Ming
关键词:
谷子; 草甘膦; EPSPS基因; 表达载体
Keywords:
-
分类号:
Q78
DOI:
-
文献标志码:
A
摘要:
5-烯醇式丙酮酸莽草酸-3-磷酸合酶(EPSPS)是植物莽草酸合成途径中的一个重要酶，与芳香族氨基酸及其次生代谢物的合成有关。利用RT-PCR方法，从谷子中分离到5-烯醇式丙酮酸莽草酸-3-磷酸合酶基因SiEPSPS，全长1 573 bp，开放阅读框1 536 bp，编码511个氨基酸。同源序列比较发现，SiEPSPS氨基酸序列与小麦、短柄草、高粱的EPSPS序列高度同源。结构同源性分析表明，SiEPSPS与其他植物一样，均具有2个序列保守的结构域。对其启动子序列分析显示，该片段富含ABRE、TC-rich repeats、TCA-element等响应胁迫的顺式作用元件。针对草甘膦结合位点，对SiEPSPS基因进行定点修饰。为研究其功能，构建植物表达载体pWM101-EPSPS，并导入农杆菌进行检测。测序结果比对表明，突变型EPSPS基因在173位的脯氨酸变为丝氨酸，为后续EPSPS的真核表达奠定了基础。
Abstract:
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备注/Memo

备注/Memo:
收稿日期：2016-02-17
基金项目：山西省青年科技研究基金(编号：2015021142)；山西农业大学科技创新基金(编号：20142-12)。
作者简介：段明(1984—)，男，山东威海人，博士，讲师，主要从事植物抗逆分子生物学研究。E-mail：duanming840305@163.com。
更新日期/Last Update: 2016-05-25