|本期目录/Table of Contents|

[1]王军华,王易芬,陈蕾蕾,等.除草剂草甘膦微生物降解技术研究进展[J].江苏农业科学,2016,44(04):8-12.
　Wang Junhua,et al.Research progress on microbial degradation of glyphosate[J].Jiangsu Agricultural Sciences,2016,44(04):8-12.
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第44卷
期数:
2016年04期
页码:
8-12
栏目:
专论
出版日期:
2016-04-25

文章信息/Info

Title:
Research progress on microbial degradation of glyphosate
作者:
王军华;  王易芬;  陈蕾蕾;  周庆新;  杜方岭
山东省农业科学院农产品研究所，山东济南 250100
Author(s):
Wang Junhua; et al
关键词:
草甘膦; 微生物降解; 降解途径; 原位修复
Keywords:
-
分类号:
S482.4；S182
DOI:
-
文献标志码:
A
摘要:
草甘膦是目前世界上使用量最大的除草剂品种之一，造成日益严重的环境污染和生态危害，并随着食物链在哺乳动物体内富集，进而危害人类健康；微生物降解是清除环境中草甘膦的最主要途径，是降低草甘膦危害的一种有效方法。对近几年筛选得到的草甘膦高效降解菌、主要降解途径和降解产物、草甘膦的原位修复研究进行了总结，探讨了微生物降解用于缓解草甘膦造成的环境污染的难题。
Abstract:
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参考文献/References:

［1］徐杰，蒋世云，傅凤鸣，等. EPSP合酶的研究进展［J］. 生物技术通报，2014(6)：40-50.
［2］向文胜，张文吉，王相晶，等. EPSP合成酶的特性及新抑制剂的研究进展［J］. 农药学学报，2000，2(2)：1-8.
［3］Williams G M，Kroes R，Munro I C. Safety evaluation and risk assessment of the herbicide Roundup and its active ingredient，glyphosate，for humans［J］. Regulatory Toxicology and Pharmacology，2000，31(2)：117-165.
［4］王慧，闫晓红，徐 杰，等. 我国抗草甘膦基因的发掘现状［J］. 农业生物技术学报，2014，22(1)：109-118.
［5］周垂帆，李莹，张晓勇，等. 草甘膦毒性研究进展［J］. 生态环境学报，2013，22(10)：1737-1743.
［6］刘攀. 草甘膦对土壤微生态的影响及其抗性和降解真菌的研究［D］. 长春：吉林大学，2009.
［7］邓晓，李雅琦. 草甘膦对土壤微生物影响的研究［J］. 农药，2005，44(2)：59-62.
［8］范瑾煜. 水环境中低浓度草甘膦及制剂对鲫鱼的毒性效应研究［D］. 南京：南京大学，2013.
［9］Aparicio V C，de Geronimo E，Marino D A，et al. Environmental fate of glyphosate and aminomethylphosphonic acid in surface waters and soil of agricultural basins［J］. Chemosphere，2013，93(9)： 1866-1873.
［10］Marc J，Mulner-Lorillon O，Boulben S，et al. Pesticide roundup provokes cell division dysfunction at the level of CDK1/cyclin B activation［J］. Chemical Research in Toxicology，2002，15(3)：326-331.
［11］Paganelli A，Gnazzo V，Acosta H，et al. Glyphosate-Based herbicides produce teratogenic effects on vertebrates by impairing retinoic acid signaling［J］. Chemical Research in Toxicology，2010，23(10)： 1586-1595.
［12］Clair E，Mesnage R，Travert C，et al. A glyphosate-based herbicide induces necrosis and apoptosis in mature rat testicular cells in vitro，and testosterone decrease at lower levels［J］. Toxicology in Vitro，2012，26(2)：269-279.
［13］Poulsen M S，Rytting E，Mose T，et al. Modeling placental transport： Correlation of in vitro BeWo cell permeability and ex vivo human placental perfusion［J］. Toxicology in Vitro，2009，23(7)：1380-1386.
［14］卢信，赵炳梓，张佳宝，等. 除草剂草甘膦的性质及环境行为综述［J］. 土壤通报，2005，36(5)：785-790.
［15］Araújo A F，Monteiro R R，Abarkeli R B. Effect of glyphosate on the microbial activity of two Brazilian soils［J］. Chemosphere，2003，52(5)：799-804.
［16］Forlani G，Mangiagalli A，Nielsen E，et al. Degradation of the phosphonate herbicide glyphosate in soil： evidence for a possible involvement of unculturable microorganisms［J］. Soil Biology & Biochemistry，1999，31(7)： 991-997.
［17］Moore J K，Braymer H D，Larson A D. Isolation of a Pseudomonas sp. which utilizes the phosphonate herbicide glyphosate［J］. Applied and Environmental Microbiology，1983，46(2)： 316-320.
［18］Shinabarger D L，Braymer H D. Glyphosate catabolism by Pseudomonas sp. strain pg2982［J］. Journal of Bacteriology，1986，168(2)： 702-707.
［19］Jacob G S，Garbow J R，Hallas L E，et al. Metabolism of glyphosate in Pseudomonas sp. strain LBr［J］. Applied and Environmental Microbiology，1988，54(12)：2953-2958.
［20］Weidhase R，Albrecht B，Stock M，et al. Glyphosate utilization by Pseudomonas spec. GS［J］. Zentralblatt Fur Mikrobiologie，1990，145(6)：433-438.
［21］Zboinska E，Lejczak B，Kafarski P. Organophonate utilization by the wild-type strain of Psrudomonas fluorrsens［J］. Applied and Environmental Microbiology，1992，58(9)：2993-2999.
［22］Penalozavazquez A，Mena G L，Herreraestrella L，et al. Cloning and sequencing of the genes involved in glyphosate utilization by Pseudomonas pseydinallei［J］. Applied and Environmental Microbiology，1995，61(2)：538-543.
［23］Talbot H W，Johnson L M，Munnecke D M. Glyphosate utilization by Pseudomonas sp. and Alcaligenes sp. isolated from environmental sources［J］. Current Microbiology，1984，10(5)：255-259.
［24］Balthazor T M，Hallas L E. Glyphosate-degrading microorganisms from industrial activated-sludge［J］. Applied and Environmental Microbiology，1986，51(2)：432-434.
［25］Pipke R，Amrhein N，Jacob G S，et al. Metabolism of glyphosate in an Arthrobacter sp. GLP-1［J］. European Journal of Biochemistry，1987，165(2)：267-273.
［26］Pipke R A N，Characterization of a mutant of Arthrobacter sp. strain GLP-1 which utilizes the herbicide glyphosate as its sole source of phosphorus and nitrogen［J］. Applied and Environmental Microbiology，1988，54(11)： 2868-2870.
［27］Liu C M，Mclean P A，Sookdeo C C，et al. Degradation of the herbicide glyphosate by members of the family rhizobiaceae［J］. Applied and Environmental Microbiology，1991，57(6)： 1799-1804.
［28］Krzysko L T，Orlik A. The use of glyphosate as the sole source of phosphorus or carbon for the selection of soil-borne fungal strains capable to degrade this herbicide［J］. Chemosphere，1997，34(12)： 2601-2605.
［29］Romero M C，Reinoso E H，Kiernan A M，et al. Biodegradation of glyphosate by wild yeasts［J］. Revista Mexicana de Micologia，2004，19： 45-50.
［30］Klimek M，Lejczak B，Kafarski P，et al. Metabolism of the phosphonate herbicide glyphosate by a non-nitrate-utilizing strain of Penicillium chrysogenum［J］. Pest Management Science，2001，57(9)： 815-821.
［31］Obojska A，Lejczak B，Kubrak M. Degradation of phosphonates by streptomycete isolates［J］. Applied Microbiology and Biotechnology，1999，51(6)： 872-876.
［32］潘渠，杨志荣. 一株降解草甘磷的真菌分离鉴定［J］. 四川大学学报：自然科学版，2001，38(1)：131-133.
［33］石成春，郭养浩，王大奈，等. 草甘膦曲霉生物降解的动力学研究［J］. 中国环境科学，2005，25(3)：361-365.
［34］Sviridov A V，Shushkova T V，Zelenkova N F，et al. Distribution of glyphosate and methylphosphonate catabolism systems in soil bacteria Ochrobactrum anthropi and Achromobacter sp.［J］. Applied Microbiology and Biotechnology，2012，93(2)： 787-796.
［35］Hadi F，Mousavi A，Noghabi K A，et al. New bacterial strain of the genus Ochrobactrum with glyphosate-degrading activity［J］. Journal of Environmental Science and Health. Part B，Pesticides，Food Contaminants，and Agricultural Wastes，2013，48(3)： 208-213.
［36］康纪婷，吴翔，甘炳成，等. 高效有机磷降解菌BR13和BR57的分离和鉴定［J］. 西南农业学报，2011，24(2)：566-569.
［37］Selvi A A，Manonmani H K. Purification and characterization of carbon-phosphorus bond-cleavage enzyme from glyphosate degrading pseudomonas putida T5［J］. Preparative Biochemistry & Biotechnology，2015，45(4)：380-397.
［38］Moneke A N，Okpala G N，Anyanwu C U. Biodegradation of glyphosate herbicide in vitro using bacterial isolates from four rice fields［J］. African Journal of Biotechnology，2010，9(26)：4067-4074.
［39］Kryuchkova Y V，Burygin G L，Gogoleva N E，et al. Isolation and characterization of a glyphosate-degrading rhizosphere strain，Enterobacter cloacae K7［J］. Microbiological Research，2014，169(1)：99-105.
［40］Fan J，Yang G，Zhao H，et al. Isolation，identification and characterization of a glyphosate-degrading bacterium，Bacillus cereus CB4，from soil［J］. Journal of General and Applied Microbiology，2012，58(4)：263-271.
［41］Zhan T，Zhang K，Chen Y Y，et al. Improving glyphosate oxidation activity of glycine oxidase from bacillus cereus by directed evolution［J］. PLoS One，2013，8(11)：e79175.
［42］韩丽珍，刘飞，赵德刚. 1株草甘膦降解菌的分离鉴定及特性［J］. 贵州农业科学，2012，40(12)：139-142，145.
［43］叶明，陈九山，姚晓庆. 一株草甘膦降解菌分离鉴定及其降解特性研究［J］. 环境科学与技术，2009，32(3)：39-41.
［44］李冠喜，吴小芹，叶建仁. 多噬伯克霍尔德氏菌WS-FJ9对草甘膦的降解特性［J］. 生态学报，2013，33(21)：6885-6894.
［45］汤鸣强，孙丽花. 酵母菌S-2对草甘膦除草剂的降解特性［J］. 安徽农业科学，2010，38(4)：1992-1994.
［46］汤鸣强，尤民生. 抗草甘膦酵母菌ZM-1的分离鉴定及其生长降解特性［J］. 微生物学通报，2010，37(9)：1402-1409.
［47］Wu X H，Fu G M，Wan Y，et al. Isolation and identification of glyphosate-degraded strain Aspergillus oryzae sp. A-F02 and its degradation characteristics［J］. Plant Diseases and Pests，2010，1(5)：54-57.
［48］Kishore G M，Barry G F. Glyphosate tolerant plants：WO92/00377 ［P］. 1992-01-09.
［49］Kononova S V，Nesmeyanova M A. Phosphonates and their degradation by microorganisms［J］. Biochemistry，2002，67(2)：184-195.
［50］Kishore G M，Jacob G S. Degradation of glyphosate by Pseudomonas sp. PG2982 via a sarcosine intermediate［J］. Journal of Biological Chemistry，1987，262(25)：12164-12168.
［51］Avila L Z，Loo S H，Frost J W. Chemical and mutagenic analysis of aminomethylphosphonate biodegradation［J］. Journal of the American Chemical Society，1987，109(22)：6758-6764.
［52］Balthazor T M，Hallas L E. Glyphosate-Degrading microorganisms from industrial activated sludge［J］. Applied and Environmental Microbiology，1986，51(2)：432-434.
［53］Dick R E，Quinn J P. Glyphosate-Degrading isolates from environmentel-samples-occurrence and pathways of degradation［J］. Applied Microbiology and Biotechnology，1995，43(3)：545-550.
［54］Bazot S，Lebeau T. Simultaneous mineralization of glyphosate and diuron by a consortium of three bacteria as free-and/or immobilized-cells formulations［J］. Applied Microbiology and Biotechnology，2008，77(6)：1351-1358.
［55］Jacob G S，Schaefer J，Stejskal E O，et al. Solid-state NMR determination of glyphosate metabolism in a Pseudomonas sp.［J］. Journal of Biological Chemistry，1985，260(10)： 5899-5905.
［56］Sviridov A V，Zelenkova N F，Vinokurova N G，et al. New approaches to identification and activity estimation of glyphosate degradation enzymes［J］. Biochemistry，2011，76(6)： 720-725.
［57］Tomita M，Okuyama T，Watanabe S，et al. High-performance liquid chromatographic determination of glyphosate and (aminomethyl) phosphonic acid in human serum after conversion into p-toluenesulphonyl derivatives［J］. Journal of Chromatography，1991，566(1)： 239-243.
［58］Roy D N，Konar S K. Development of an analytical method for the determination of glyphosate and (aminomethyl) phosphonic acid residues in soils by nitrogen-selective gas chromatography［J］. Journal of Agricultural and Food Chemistry，1989，37(2)： 441-443.
［59］Alferness P L，Iwata Y A. Phosphonic acid in Soil，plant and animal matrixes，and water by capillary gas chromatography with mass-selective detection［J］. Journal of Agricultural and Food Chemistry，1994，42(12)： 2751-2759.
［60］Kudzin Z H，Gralak D K，Drabowicz J，et al. Novel approach for the simultaneous analysis of glyphosate and its metabolites［J］. Journal of Chromatography，2002，947(1)： 129-141.
［61］王彦辉，李欣，周小毛，等. 草甘膦铵盐在苎麻田的残留及消解动态［J］. 农药学学报，2010，12(2)：201-206.
［62］Zelenkova N F，Vinokurova N G，Leontievskii A A. Determination of Amine-Containing phosphonic acids and amino acids as dansyl derivatives［J］. Journal of Analytical Chemistry，2010，65(11)： 1143-1147.
［63］Tomita M，Okuyama T，Nigo Y，et al. Determination of glyphosate and its metabolite，(aminomethyl)phosphonic acid，in serum using capillary electrophoresis［J］. Journal of Chromatography，1991，571(1/2)： 324-330.
［64］曹丽伟，梁丝柳，谭小芳，等. 毛细管电泳-激光诱导荧光法测定草甘膦、草胺膦及氨甲基膦酸［J］. 色谱，2012，30(12)：1295-1300.
［65］Veiga F，Zapata J M，Marcos M F，et al. Dynamics of glyphosate and aminomethyl phosphonic acid in a forest soil in Galicia，north-west Spain［J］. Science of the Total Environment，2001，271(1/2/3)： 135-144.
［66］Sorensen S R，Schultz A，Jacobsen O S，et al. Sorption，desorption and mineralisation of the herbicides glyphosate and MCPA in samples from two Danish soil and subsurface profiles［J］. Environmental Pollution，2006，141(1)： 184-194.
［67］Ermakova I T，Kiseleva N I，Shushkova T A，et al. Bioremediation of glyphosate-contaminated soils［J］. Applied Microbiology and Biotechnology，2010，88(2)：585-594.
［68］Shushkova T，Ermakova I，Leontievsky A. Glyphosate bioavailability in soil［J］. Biodegradation，2010，21(3)：403-410.

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备注/Memo

备注/Memo:
收稿日期：2015-03-19
基金项目：国家国际科技合作专项(编号：2013DFR30920)。
作者简介：王军华(1985—)，女，博士，助理研究员，主要从事食品微生物基础研究。E-mail：wangjunhua85@126.com。
通信作者：杜方岭，研究员，主要研究方向为农产品加工。E-mail: dufl@saas.ac.cn。
更新日期/Last Update: 2016-04-25