|本期目录/Table of Contents|

[1]张洁.干旱盐碱共胁迫下玉米miR398的表达[J].江苏农业科学,2016,44(07):45-47.
 Zhang Jie.Expression profile of miR398 in maize under co-stress of drought and salinity[J].Jiangsu Agricultural Sciences,2016,44(07):45-47.
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干旱盐碱共胁迫下玉米miR398的表达(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第44卷
期数:
2016年07期
页码:
45-47
栏目:
生物技术
出版日期:
2016-07-25

文章信息/Info

Title:
Expression profile of miR398 in maize under co-stress of drought and salinity
作者:
张洁
南通大学农业部南方平原玉米科学观测实验站,江苏南通 226001
Author(s):
Zhang Jie
关键词:
玉米干旱盐碱miR398
Keywords:
-
分类号:
S513.032
DOI:
-
文献标志码:
A
摘要:
分析干旱盐碱共胁迫下玉米miR398的表达变化,阐明miR398与玉米水势胁迫生理指标关系。干旱盐碱共胁迫处理72 h,利用qRT-PCR技术分别验证了miR398及其他对应的靶基因在盐胁迫条件下的表达变化情况;应用ELISA和茚三铜方法分别检测玉米的ABA (脱落酸)和脯氨酸含量。结果表明:经过干旱盐碱共胁迫处理,玉米出现了明显的水分亏缺症状,24、48、72 h各处理组玉米ABA和脯氨酸含量与对照组比较上升明显,差异有统计学意义(P<0.05);RT-PCR检测显示,24、48、72 h各处理组玉米中miR398的表达比对照组显著降低,差异有统计学意义(P<0.05)。说明干旱盐碱共胁迫条件下玉米miR398表达下调,可能参与抗逆相关基因ABA的调控。试验为后续抗旱抗盐碱玉米遗传性状研究提供了基础数据。
Abstract:
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参考文献/References:

[1]Gwirtz J A,Garcia-Casal M N. Processing maize flour and corn meal food products[J]. Annals of the New York Academy of Sciences,2014,1312:66-75.
[2]Ciniglia C,Mastrobuoni F,Scortichini M,et al. Oxidative damage and cell-programmed death induced in Zea mays L. by allelochemical stress [J]. Ecotoxicology,2015,24(4):926-937.
[3]张正斌,徐萍,张建华,等. 作物抗旱节水相关基因的标记和克隆及转基因研究进展[J]. 西北植物学报,2002,22(6):1537-1544.
[4]Ferdous J,Li Y,Reid N,et al. Identification of reference genes for quantitative expression analysis of microRNAs and mRNAs in barley under various stress conditions[J]. PLoS One,2015,10(3):e0118503.
[5]Zhou M,Luo H.Role of microRNA319 in creeping bentgrass salinity and drought stress response[J]. Plant Signal Behav,2014,9(4):e28700.
[6]李绍军,龚月桦,王俊儒,等. 关于茚三酮法测定脯氨酸含量中脯氨酸与茚三酮反应之探讨[J]. 植物生理学通讯,2005,41(3):365-368.
[7]Wang Y G,An M,Zhou S F,et al. Expression profile of maize microRNAs corresponding to their target genes under drought stress[J]. Biochemical Genetics,2014,52(11/12):474-493.
[8]Syu Y Y,Hung J H,Chen J C,et al. Impacts of size and shape of silver nanoparticles on Arabidopsis plant growth and gene expression[J]. Plant Physiology and Biochemistry,2014,83:57-64.
[9]Sunkar R,Kapoor A,Zhu J K. Posttranscriptional induction of two Cu/Zn superoxide dismutase genes in Arabidopsis is mediated by downregulation of miR398 and important for oxidative stress tolerance[J]. The Plant Cell,2006,18(8):2051-2065.
[10]Zhao M,Tai H,Sun S,et al. Cloning and characterization of maize miRNAs involved in responses to nitrogen deficiency[J]. PLoS One,2012,7(1):e29669.
[11]冯艳,周蓉,付蓉,等. 玉米遗传多样性分析及耐盐种质筛选[J]. 江苏农业科学,2015,43(7):59-61.
[12]汤菊香,赵元增,单长卷. 水杨酸对盐胁迫下新单29玉米幼苗生理特性的影响[J]. 江苏农业科学,2015,43(6):93-95.
[13]岳润清,铁双贵,韩小花,等. 农杆菌介导的转拟南芥AtCHX23基因玉米及其耐盐性[J]. 江苏农业学报,2014,30(4):726-732.
[14]刘芳,付艳,高树仁,等. 玉米幼苗的盐胁迫反应及玉米耐盐性的鉴定[J]. 黑龙江八一农垦大学学报,2007,19(6):22-26.
[15]崔美燕. 玉米苗期耐碱种质资源评价及盐碱条件下部分性状遗传分析[D]. 大庆:黑龙江八一农垦大学,2009.
[16]付艳,高树仁,王振华. 玉米种质苗期耐盐性的评价[J]. 玉米科学,2009,17(1):36-39,50.

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备注/Memo

备注/Memo:
收稿日期:2015-04-30
基金项目:农业部南方平原玉米科学观测实验站开放课题(编号:NT201514)。
作者简介:张洁(1980—),男,安徽舒城人,博士,副教授,主要从事玉米表观遗传学研究。E-mail:zhangjie@ntu.edu.cn。
更新日期/Last Update: 2016-07-25