«上一篇/Previous Article|本期目录/Table of Contents|下一篇/Next Article»

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:

第44卷

期数:

2016年08期

页码:

47-50

栏目:

生物技术

出版日期:

2016-08-25

文章信息/Info

Title:

Bioinformatics analysis of Capra hircus PMEL gene

作者:

李丽莎¹;  李祥龙¹; 2;  毛艳朋¹

1.河北农业大学，河北保定 071000；2.河北科技师范学院，河北秦皇岛 066004

Author(s):

Li Lisha; et al

关键词:

PMEL; 山羊; 生物信息

Keywords:

-

分类号:

Q785；S826.9⁺2

DOI:

-

文献标志码:

A

摘要:

基于生物基因组学数据库，对山羊PMEL基因进行生物信息学分析，为研究该基因的功能提供依据。根据GenBank中山羊PMEL基因编码蛋白序列(GenBank登录号：XP_005680442.1)，利用ExPASy数据库中的ProtParam、ProtScale程序分别预测理化性质、疏水性；利用CBS Prediction Servers数据库中的SignalP、TMHMM、Protfun程序分别预测信号肽、跨膜区及其功能；利用Predict Protein、PSORT Ⅱ Prediction、Motif Scan软件分别预测二级结构、亚细胞定位、功能位点，并对不同物种PMEL基因构建系统发育树。结果表明：山羊PMEL基因共编码685个氨基酸，属可溶性不稳定蛋白，无信号肽，在接近肽链的C-端存在1个跨膜区；二级结构以无规卷曲为主，该蛋白主要在内质网中发挥运输、结合、信号转导作用；不同物种PMEL基因的系统进化情况与生物进化过程中的动物学分类相符。山羊PMEL基因编码蛋白在生物进化中具有较强保守性，该基因结构和功能的分析为山羊PMEL基因遗传特性的进一步研究奠定了基础。

Abstract:

-

参考文献/References:

［1］Watt B，Raposo G，Marks M S. PMEL17：an amyloid determinant of organelle structure［M］//Rigacci S，Bucciantini M. Functional amyloid aggregation. Trivandrum，Kerala，India：Research Signpost，2010：89-113.
［2］Brouwenstijn N，Slager E H，Bakker A B，et al. Transcription of the gene encoding melanoma-associated antigen gp100 in tissues and cell lines other than those of the melanocytic lineage［J］. British Journal of Cancer，1997，76(12)：1562-1566.
［3］Leonhardt R M，Vigneron N，Hee J S，et al. Critical residues in the PMEL/Pmel17 N-terminus direct the hierarchical assembly of melanosomal fibrils［J］. Molecular Biology of the Cell，2013，24(7)：964-981.
［4］Kwon B S，Halaban R，Kim G S，et al. A melanocyte-specific complementary DNA clone whose expression is inducible by melanotropin and isobutylmethyl xanthine［J］. Molecular Biology & Medicine，1987，4(6)：339-355.
［5］Berson J F，Harper D C，Tenza D，et al. Pmel17 initiates premelanosome morphogenesis within multivesicular bodies［J］. Molecular Biology of the Cell，2001，12(11)：3451-3464.
［6］Solano F，Martínez E M，Jiménez C C，et al. New insights on the structure of the mouse silver locus and on the function of the silver protein［J］. Pigment Cell Research，2000，13(Suppl 8)：118-124.
［7］呼蕾，孙丽君，李元，等. 白癜风患者血清前黑素小体蛋白PMEL17自身抗体的检测［J］. 细胞与分子免疫学杂志，2013，29(4)：411-413.
［8］Martnez E M，Jimnez C C，Solano F，et al. The murine silver locus：coding and expression of a single transcript truncated by the silver mutation［J］. Mamm Genome，1999，10：1168-1171.
［9］Brunberg E，Andersson L，Cothran G，et al. A missense mutation in PMEL17 is associated with the silver coat color in the horse［J］. BMC Genetics，2006，7：46.
［10］Mészáros G，Petautschnig E，Schwarzenbacher H，et al. Genomic regions influencing coat color saturation and facial markings in Fleckvieh cattle［J］. Animal Genetics，2015，46(1)：65-68.
［11］Menotti R M，David V A，Eizirik E，et al. Mapping of the domestic cat “Silver” coat color locus identifies a unique genomic location for Silver in mammals［J］. The Journal of Heredity，2009，100(Suppl 1)：8-13.
［12］Kerje S，Sharma P，Gunnarsson U，et al. The dominant white，dun and smoky color variants in chicken are associated with insertion/deletion polymorphisms in the PMEL17 gene［J］. Genetics，2004，168(3)：1507-1518.
［13］Mcglinchey R P，Shewmaker F，Mcphie P，et al. The repeat domain of the melanosome fibril protein Pmel17 forms the amyloid core promoting melanin synthesis［J］. Proceedings of the National Academy of Sciences of the United States of America，2009，106(33)：13731-13736.
［14］Commo S，Bernard B A. Melanocyte subpopulation turnover during the human hair cycle：an immunohistochemical study［J］. Pigment Cell Research，2000，13(4)：253-259.
［15］Schmutz S M，Dreger D L. Interaction of MC1R and PMEL alleles on solid coat colors in Highland cattle［J］. Animal Genetics，2013，44(1)：9-13.
［16］王明亮，陈鹏，郝明超，等. 黄牛FSHR基因的生物信息学分析［J］. 甘肃农业大学学报，2012，47(4)：7-13.
［17］Adema G J，de Boer A J，Vogel A M，et al. Molecular characterization of the melanocyte lineage-specific antigen gp100［J］. The Journal of Biological Chemistry，1994，269(31)：20126-20133.
［18］Maresh G A，Wang W C，Beam K S，et al. Differential processing and secretion of the melanoma-associated ME20 antigen［J］. Archives of Biochemistry and Biophysics，1994，311(1)：95-102.

相似文献/References:

[1]胡新岗,黄银云.1例引入性波尔杂交羊传染性胸膜肺炎的诊治[J].江苏农业科学,2013,41(04):189.
[2]卢炜,张磊,刘静,等.口服半胱胺对肉羊生产性能和血清激素水平的影响[J].江苏农业科学,2014,42(11):241.
　Lu Wei,et al().Effects of oral cysteamine on production performance and serum hormone levels of mutton sheep[J].Jiangsu Agricultural Sciences,2014,42(08):241.
[3]宋德荣,王嘉福,周大荣,等.贵州黑马羊新品种培育[J].江苏农业科学,2013,41(06):163.
　Song Derong,et al.Breeding of new Guizhou dark horse sheep variety[J].Jiangsu Agricultural Sciences,2013,41(08):163.
[4]劳雪芬,曹铮,汤里平,等.富硒女贞子对山羊生产性能、血液学和血清生化指标的影响[J].江苏农业科学,2016,44(03):256.
　Lao Xuefen,et al.Effects of Se-enriched Ligustrum lucidum fruits on performance，hematology and serum biochemical indices of goats[J].Jiangsu Agricultural Sciences,2016,44(08):256.
[5]骆美蓉,江明锋,张鹏,等.山羊分子生物学研究进展[J].江苏农业科学,2014,42(01):46.
　Luo Meirong,et al.Research progress of molecular biology of goat[J].Jiangsu Agricultural Sciences,2014,42(08):46.
[6]于鸿浩,闫海龙,朱海鲸,等.山羊卵泡数量对卵母细胞体外成熟的影响[J].江苏农业科学,2015,43(09):232.
　Yu Honghao,et al.Effect of follicle number on oocyte in vitro mature of goat[J].Jiangsu Agricultural Sciences,2015,43(08):232.
[7]胡新岗,黄银云,徐婷婷,等.江苏泰州地区山羊传染性胸膜肺炎血清学调查[J].江苏农业科学,2015,43(07):228.
　Hu Xingang,et al.Serological survey of contagious caprine pleuropneumonia in Taizhou，Jiangsu Province[J].Jiangsu Agricultural Sciences,2015,43(08):228.
[8]卢劲晔,刘静,卢炜,等.日粮能量水平对山羊瘤胃上皮细胞周期的影响[J].江苏农业科学,2014,42(06):145.
　Lu Jingye,et al.Effect of dietary energy on rumen epithelium cell cycle of goats[J].Jiangsu Agricultural Sciences,2014,42(08):145.
[9]杨家大.山羊心脏型脂肪酸结合蛋白基因mRNA的组织表达谱分析[J].江苏农业科学,2015,43(06):24.
　Yang Jiada.Analysis of tissue expression profiles of H-FABP gene in goats[J].Jiangsu Agricultural Sciences,2015,43(08):24.
[10]白云峰,高立鹏,涂远璐,等.精饲料水平对育肥山羊玉米苞叶自由采食量及生产性能的影响[J].江苏农业科学,2015,43(05):201.
　Bai Yunfeng,et al.Effect of concentrated feed levels on free intake and production performance of meat goats fed corn bract feed[J].Jiangsu Agricultural Sciences,2015,43(08):201.