|本期目录/Table of Contents|

[1]刘哲,许园园,苏小俊.萝卜抽薹相关SRAP分子标记筛选与分析[J].江苏农业科学,2016,44(08):74-76.
 Liu Zhe,et al.Screening and analysis of radish bolting related SRAP molecular markers[J].Jiangsu Agricultural Sciences,2016,44(08):74-76.
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萝卜抽薹相关SRAP分子标记筛选与分析(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第44卷
期数:
2016年08期
页码:
74-76
栏目:
生物技术
出版日期:
2016-08-25

文章信息/Info

Title:
Screening and analysis of radish bolting related SRAP molecular markers
作者:
刘哲 许园园 苏小俊
江苏省农业科学院蔬菜研究所,江苏南京 210014
Author(s):
Liu Zheet al
关键词:
萝卜晚抽薹分子标记遗传距离
Keywords:
-
分类号:
Q943;S631.103
DOI:
-
文献标志码:
A
摘要:
利用抽薹性状差异较大的晚抽薹萝卜品种L2和早抽薹萝卜品种E6配制杂交组合,构建F2群体。通过田间调查,选用极晚和极早抽薹单株分别构建晚抽池(BSA-1)和早抽池(BSA-2),用288对SRAP组合对两亲本进行PCR分析,共筛选出166对SRAP引物组合。通过2个基因池及基因池中各单株对筛选出的引物进行复筛,获得引物组合me3+em9,其在晚抽池中可扩增出多态性片段LB;对156株F2单株进行验证发现,LB与晚抽薹基因紧密连锁,其遗传距离为5.7 cM。
Abstract:
-

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2015-06-15
基金项目:“十二五”农村领域国家科技计划(编号:2012BAD02B01);江苏省科技支撑计划(编号:BE2013429);江苏省农业科技自主创新资金[编号:CX(12)2006]。
作者简介:刘哲(1987—),男,山东曹县人,硕士,主要从事十字花科蔬菜遗传育种研究。E-mail:641509581@qq.com。
通信作者:苏小俊,博士,研究员,主要从事瓜类和十字花科蔬菜遗传育种研究。Tel:(025)84391259;E-mail:xiaojunsu@yahoo.com。
更新日期/Last Update: 2016-08-25