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[1]丛汉卿,齐尧尧,朱文丽,等.木薯PHOR1基因的序列分析及其乙烯和茉莉酸甲酯诱导表达特性[J].江苏农业科学,2016,44(11):29-33.
　Cong Hanqing,et al.Sequence analysis of PHOR1 gene and its expression characteristics induced by ethylene and methyl jasmonate in Manihot esculenta Crantz[J].Jiangsu Agricultural Sciences,2016,44(11):29-33.
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木薯PHOR1基因的序列分析及其乙烯
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第44卷
期数:
2016年11期
页码:
29-33
栏目:
生物技术
出版日期:
2016-11-25

文章信息/Info

Title:
Sequence analysis of PHOR1 gene and its expression characteristics induced by ethylene and methyl jasmonate in Manihot esculenta Crantz
作者:
丛汉卿; 齐尧尧; 朱文丽; 陈松笔; 李丽
中国热带农业科学院热带作物品种资源研究所/农业部热带作物种质资源利用重点开放实验室，海南儋州 571737
Author(s):
Cong Hanqing; et al
关键词:
木薯; PHOR1基因; 乙烯; 茉莉酸; 表达分析
Keywords:
-
分类号:
S533.01
DOI:
-
文献标志码:
A
摘要:
为研究木薯中PHOR1基因如何被逆境信号调控，根据杨树2个PHOR1同源基因搜索木薯基因组数据库，得到2个高度同源的木薯PHOR1基因，即MePHOR1_1和MePHOR1_2。序列分析表明，它们都具有E3泛素连接酶家族成员特征：含有U-box和armadillo重复序列。此外其启动子区具有乙烯响应元件和茉莉酸响应元件。以木薯品种华南八号悬浮培养细胞为材料，利用qRT-PCR检测MePHOR1_1和MePHOR1_2基因在乙烯利和茉莉酸甲酯处理后的表达特性。结果显示：在乙烯和茉莉酸甲酯分别处理后的细胞悬浮液中，2个PHOR1基因的表达变化呈现相似趋势，即乙烯处理后，2个基因的表达均出现先下降后缓慢上升的趋势；而对茉莉酸信号则呈现先上升后下降又上升再下降的波动性趋势，故推测2个PHOR1基因可被乙烯和茉莉酸信号调控，并可在后续的根生长和淀粉积累等一系列生理生化过程中发挥作用。
Abstract:
-

参考文献/References:

［1］Amador V，Monte E，Martínez-García J L，et al. Gibberellins signal nuclear import of PHOR1，a photoperiod-responsive protein with homology to Drosophila armadillo［J］. Cell，2001，106(3)：343-354.
［2］Olszewski N，Sun T P，Gubler F. Gibberellin signaling：biosynthesis，catabolism，and response pathways［J］. Plant Cell，2002，14：S61-S80.
［3］Monte E，Amador V，Russo E，et al. PHOR1，a U-Box GA signaling component with a role in proteasome degradation? ［J］. Journal of Plant Growth Regulation，2003，22：152-162.
［4］Pringa E，Martinez-Noel G，Muller U，et al. Interaction of the RING finger-related U-box motif of a nuclear dot protein with ubiquitin-conjugating enzymes［J］. Journal of Biological Chemistry，2001，276：19617-19623.
［5］Conconi A，Smerdon M J，Howe G A，et al. The octadecanoid signalling pathway in plants mediates a response to ultraviolet radiation［J］. Nature，1991，383(6603)：826-829.
［6］Turner J G，Ellis C，Devoto A. The jasmonate signal pathway［J］. Plant Cell，2002，14(Suppl1)：S153-S164.
［7］Bleecker B，Kende H. Ethylene：a gaseous signal molecule in plants［J］. Annual Review of Cell and Developmental Biology，2000，16：1-18.
［8］Johnson P R，Ecker J R. The ethylene gas signal transduction pathway：a molecular perspective［J］. Annual Review of Genetics，1998，32：227-254.
［9］Zawaski C，Ma C，Strauss S H，et al. PHOTOPERIOD RESPONSE 1 (PHOR1) -like genes regulate shoot/root growth，starch accumulation，and wood formation in Populus［J］. Journal of Experimental Botany，2012，63(15)：5623-5634.
［10］Prochnik S，Marri P R，Desany B，et al. The cassava genome：current progress，future directions［J］. Tropical Plant Biology，2012，5：88-94.
［11］Brunak S，Engelbrecht J，Knudsen S. Prediction of human mRNA donor and acceptor sites from the DNA sequence［J］. Journal of Molecular Biology，1991，220：49-65.
［12］Hebsgaard S M，Korning P G，Tolstrup N，et al. Splice site prediction in Arabidopsis thaliana pre-mRNA by combining local and global sequence information［J］. Nucleic Acids Research，2010，24(17)：3439-3452.
［13］Hu B，Jin J P，Guo A Y，et al. GSDS 2.0：an upgraded gene feature visualization server［J］. Bioinformatics，2015，31(8)：1296-1297.
［14］Solovyev V V，Shahmuradov I A. PromH：Promoters identification using orthologous genomic sequences［J］. Nucleic Acids Research，2003，31(13)：3540-3545.
［15］Lescot M，Déhais P，Moreau Y，et al. PlantCARE，a database of plant cis-acting regulatory elements and a portal to tools for in silico analysis of promoter sequences［J］. Nucleic Acids Research，2002，30(1)：325-327.
［16］Gasteiger E，Gattiker A，Hoogland C，et al. ExPASy：the proteomics server for in-depth protein knowledge and analysis［J］. Nucleic Acids Research，2003，31(13)：3784-3788.
［17］Hunter S，Apweiler R，Attwood T K，et al. InterPro：the integrative protein signature database［J］. Nucleic Acids Research，2009，37(Database issue)：D211-D215.
［18］Bailey T L，Elkan C. Fitting a mixture model by expectation maximization to discover motifs in biopolymers［J］. Intell Syst Mol Biol，1994，2：28-36.
［19］Bailey T L，Gribskov M. Combining evidence using p-values：application to sequence homology searches［J］. Bioinformatics，1998，14(1)：48-54.
［20］Krogh A，Larsson B，Von Heijne G，et al. Predicting transmembrane protein topology with a hidden Markov model：application to complete genomes［J］. Journal of Molecular Biology，2001，305：567-580.
［21］Hua S J，Sun Z R. Support vector machine approach for protein subcellular localization prediction［J］. Bioinformatics，2015，17(8)：721-728.
［22］Jones D T. Protein secondary structure prediction based on position-specific scoring matrices［J］. Journal of Molecular Biology，1999，292：195-202.
［23］Arnold K，Bordoli L，Kopp J，et al. The SWISS-MODEL Workspace：a web-based environment for protein structure homology modelling［J］. Bioinformatics，2006，22：195-201.
［24］Khan I K，Wei Q，Chitale M，et al. PFP/ESG：automated protein function prediction servers enhanced with Gene Ontology visualization tool［J］. Bioinformatics，2015，31(2)：271-272.
［25］Martinelli F，Uratsu S L，Reagan R L，et al. Gene regulation in parthenocarpic tomato fruit［J］. Journal of Experimental Botany，2009，60：3873-3890.
［26］Jia T，Wei D，Meng S，et al. Identification of regulatory genes implicated in continuous flowering of longan (Dimocarpus longan L.) ［J］. PLoS One，2014，9(12)：e114568.
[HJ1.76mm] ［27］ORourke J A，Yang S S，Miller S S，et al. An RNA-Seq transcriptome analysis of orthophosphate-deficient white lupin reveals novel insights into phosphorus acclimation in plants［J］. Plant Physiology，2013，161：705-724.
［28］Hermans C，Hammond J P，White P J，et al. How do plants respond to nutrient shortage by biomass allocation? ［J］. Trends in Plant Science，2006，11：610-617.
［29］Thomas S G，Sun T P. Update on gibberellin signaling：a tale of the tall and the short［J］. Plant Physiology，2004，135：668-676.
［30］Bjorklund S，Antti H，Uddestrand I，et al. Cross-talk between gibberellin and auxin in development of Populus wood：gibberellin stimulates polar auxin transport and has a common transcriptome with auxin［J］. The Plant Journal，2007，52：499-511.
［31］Gou J Q，Strauss S H，Tsai C J，et al. Gibberellins regulate lateral root formation in Populus through interactions with auxin and other hormones［J］. Plant Cell，2010，22：623-39.
［32］Mauriat M，Sandberg L G，Moritz T. Proper gibberellin localization in vascular tissue is required to control auxin-dependent leaf development and bud outgrowth in hybrid aspen［J］. The Plant Journal，2011，67：805-816.
［33］曾文丹，罗兴录. 2个淀粉含量不同木薯品种SSⅡ基因序列及不同生育期淀粉含量比较［J］. 江苏农业科学，2015，43(5)：35-38.
［34］安飞飞，简纯平，杨摇龙，等. 木薯幼苗叶绿素含量及光合特性对盐胁迫的响应［J］. 江苏农业学报，2015，31(3)：500-504.

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备注/Memo

备注/Memo:
收稿日期：2015-11-02
基金项目：中央级公益性科研院所基本科研业务费专项资金(编号：1630032014036)。
作者简介：丛汉卿(1983—)，男，山东潍坊人，博士，助理研究员，主要从事分子生物学和生物信息学研究。E-mail：hiigara@yeah.net。
通信作者：李丽，女，湖北天门人，博士，副研究员，主要从事分子生物学研究。E-mail：chenli907@163.com。
更新日期/Last Update: 2016-11-25