|本期目录/Table of Contents|

[1]刘斯超,许涛,董秀芬,等.番茄SlIAA16沉默转基因植株的获得及功能初探[J].江苏农业科学,2017,45(05):29-33.
　Liu Sichao,et al.Acquisition of transgenic tomato plant with SlIAA16 gene silencing and preliminary investigation on its function[J].Jiangsu Agricultural Sciences,2017,45(05):29-33.
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番茄SlIAA16沉默转基因植株的获得及功能初探(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第45卷
期数:
2017年05期
页码:
29-33
栏目:
生物技术
出版日期:
2017-03-05

文章信息/Info

Title:
Acquisition of transgenic tomato plant with SlIAA16 gene silencing and preliminary investigation on its function
作者:
刘斯超1; 2; 许涛1; 董秀芬1; 付欣1; 郑鹏靖2; 李兵2
1.沈阳农业大学园艺学院，辽宁沈阳 110161； 2.河北省承德市蔬菜技术推广站，河北承德 067000
Author(s):
Liu Sichao; et al
关键词:
番茄; 生长素; SlIAA16; 载体构建; 基因表达
Keywords:
-
分类号:
Q785；Q786
DOI:
-
文献标志码:
A
摘要:
Aux/IAA(auxin/indole-3-acetic acid)作为生长素早期响应因子，其蛋白产物能够特异性地结合生长素响应因子(auxin response factor，ARF)，从而调控生长素响应基因的表达，在整个植物生长素信号转导过程中发挥着重要的作用，进而影响植物的生长发育。本研究通过构建沉默载体，在高效遗传转化体系下获得转基因植株，发现利用Gateway克隆技术将番茄生长素早期响应基因SlIAA16 Domain Ⅱ区部分序列连入具有正反2个attr区域并连接1个内含子的pB7GWIWG2(Ⅰ)载体，可在植株体内形成发卡结构导致植物基因沉默，成功构建基因沉默表达载体名为SlIAA RNAi；此外，抗生素喷施和PCR扩增以及实时定量检测进一步鉴定，共获得12棵SlIAA16 RNAi阳性植株。本研究结果将为进一步明确SlIAA的生物学功能和阐明生长素的作用机理提供参考。
Abstract:
-

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期：2016-04-04
基金项目：国家自然科学基金(编号：30900982)。
作者简介：刘斯超(1988—)，男，山东聊城人，硕士，从事蔬菜栽培与生理研究。E-mail：m13644976629@163.com。
更新日期/Last Update: 2017-03-05