|本期目录/Table of Contents|

[1]武慧,宋明霞,宁思淇,等.紫花苜蓿的抗菌肽基因Rev4遗传转化[J].江苏农业科学,2017,45(07):44-46.
　Wu Hui,et al.Genetic transformation of antimicrobial peptide gene Rev4] in Medicago sativa[J].Jiangsu Agricultural Sciences,2017,45(07):44-46.
点击复制

紫花苜蓿的抗菌肽基因Rev4遗传转化(PDF)
分享到：

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第45卷
期数:
2017年07期
页码:
44-46
栏目:
生物技术
出版日期:
2017-04-20

文章信息/Info

Title:
Genetic transformation of antimicrobial peptide gene Rev4] in Medicago sativa
作者:
武慧;  宋明霞;  宁思淇;  武翠玲;  周晓馥
吉林师范大学植物资源科学与绿色生产吉林省重点实验室，吉林四平 136000
Author(s):
Wu Hui; et al
关键词:
紫花苜蓿; 抗菌肽; 遗传转化
Keywords:
-
分类号:
Q785
DOI:
-
文献标志码:
A
摘要:
抗菌肽是机体先天免疫的重要组成成分，是许多生物体抵抗外来致病菌侵袭的第1道屏障。Indolicidin是一个13氨基酸残基肽，从牛的嗜中性粒细胞的胞浆小颗粒分离而来，具有广谱抗菌性，Rev4是其人工设计的反向序列。利用经典叶盘法，以根癌农杆菌A.GV3850介导紫花苜蓿遗传转化，GUS瞬时表达检测转化体系建立成功；压力筛选后叶片基因组DNA的PCR成功检测到抗菌肽基因条带，PCR产物回收测序得到13株转化紫花苜蓿。
Abstract:
-

参考文献/References:

［1］ Samac D A，Bucciarelli B，Miller S S，et al. Transgene silencing of sucrose synthase in alfalfa (Medicago sativa L.) stem vascular tissue suggests a role for invertase in cell wall cellulose synthesis［J］. BMC Plant Biology，2015，15(1)： 283.
［2］Aung B，Gruber M，Amyot L，et al. MicroRNA156 as a promising tool for alfalfa improvement［J］. Plant Biotechnology Journal，2015，13(6)： 779-790.
［3］Zhang Y M，Liu Z H，Wen Z Y，et al. The vacuolar Na+-H+ antiport gene TaNHX2 confers salt tolerance on transgenic alfalfa (Medicago sativa)［J］. Functional Plant Biology，2012，39(8)： 708-716.
［4］Zhang Y，Zhang H，Liu Z，et al. The wheat NHX antiporter gene TaNHX2 confers salt tolerance in transgenic alfalfa by increasing the retention capacity of intracellular potassium［J］. Plant Molecular Biology，2015，87(3)： 317-327.
［5］ Anupa G，Bhat M A，Srivastava A K，et al. Cationic antimicrobial peptide，magainin down-regulates secretion of pro-inflammatory cytokines by early placental cytotrophoblasts［J］. Reproductive Biology and Endocrinology，2015，13(1)： 121.
［6］Urfer M，Bogdanovic J，Lo Monte F，et al. A peptidomimetic antibiotic targets outer membrane proteins and disrupts selectively the outer membrane in escherichia coli［J］. The Journal of Biological Chemistry，2016，291(4)： 1921-1932.
［7］Chou S，Shao C，Wang J，et al. Short，multiple-stranded β-hairpin peptides have antimicrobial potency with high selectivity and salt resistance［J］. Acta Biomaterialia，2016，30： 78-93.
［8］Selsted M，Novotny M，Morris W，et al. Indolicidin，a novel bactericidal tridecapeptide amide from neutrophils［J］. The Journal of Biological Chemistry，1992，267(7)： 4292-4295.
［9］Li Q，Lawrence C B，Davies H M，et al. A tridecapeptide possesses both antimicrobial and protease-inhibitory activities［J］. Peptides，2002，23(1)： 1-6.
［10］Zhang Q，Walawage S，Tricoli D，et al. A red fluorescent protein (DsRED) from Discosoma sp. as a reporter for gene expression in walnut somatic embryos［J］. Plant Cell Reports，2015，34(5)： 861-869.
［11］Liu X，Lang S，Su L，et al. Improved agrobacterium-mediated transformation and high efficiency of root formation from hypocotyl meristem of spring Brassica napus ‘Precocity’ cultivar［J］. Genetics and Molecular Research，2015，14(4)： 16840-16855.
［12］Bowling S，Guo A，Cao H，et al. A mutation in Arabidopsis that leads to constitutive expression of systemic acquired resistance［J］. The Plant Cell，1994，6(12)： 1845-1857.
［13］Anantharaman A，Sahal D. Reverse engineering truncations of an antimicrobial peptide dimer to identify the origins of potency and broad spectrum of action［J］. Journal of Medicinal Chemistry，2010，53(16)： 6079-6088.
［14］Xing H，Lawrence C，Chambers O，et al. Increased pathogen resistance and yield in transgenic plants expressing combinations of the modified antimicrobial peptides based on indolicidin and magainin［J］. Planta，2006，223(5)： 1024-1032.

相似文献/References:

[1]朱强,邹梦辉,安黎,等.琼花对4种草坪植物的化感作用[J].江苏农业科学,2014,42(10):172.
　Zhu Qiang,et al.Allelopathy of Viburnum macrocephalum to four turfgrass plants[J].Jiangsu Agricultural Sciences,2014,42(07):172.
[2]王小山,朱平华,鲍国成,等.盐碱胁迫对紫花苜蓿根、茎和叶重要养分离子平衡的影响[J].江苏农业科学,2013,41(07):190.
　Wang Xiaoshan,et al.Effect of salt stress on important nutrient ion balance in roots，stems and leaves of Medicago sativa[J].Jiangsu Agricultural Sciences,2013,41(07):190.
[3]田福平,李锦华,张怀山,等.耐旱丰产紫花苜蓿新品系杂选1号的选育及栽培技术[J].江苏农业科学,2013,41(10):92.
　Tian Fuping,et al.Breeding and cultivation techniques of new Medicago sativa lines “Zaxuan No.1”[J].Jiangsu Agricultural Sciences,2013,41(07):92.
[4]杨杰,谷陈建,吴豪杰,等.有机肥和紫花苜蓿对长期抛荒贫瘠土壤的改良效果[J].江苏农业科学,2013,41(12):362.
　Yang Jie,et al.Comparison of improved effects of organic fertilizer and alfalfa on long-abandoned barren soils[J].Jiangsu Agricultural Sciences,2013,41(07):362.
[5]周恒,时永杰,路远,等.不同种植年限紫花苜蓿种植地土壤容重及含水量特征[J].江苏农业科学,2016,44(05):490.
　Zhou Heng,et al.Study on soil bulk density and soil water content in alfalfa field with different growing years[J].Jiangsu Agricultural Sciences,2016,44(07):490.
[6]王宏亮,李昂,那杰.抗菌肽在果蔬保鲜中的应用[J].江苏农业科学,2014,42(03):238.
　Wang Hongliang,et al.Application of antimicrobial peptides in preservation of fruits and vegetables[J].Jiangsu Agricultural Sciences,2014,42(07):238.
[7]张丽辉,贾泽君,孙奇,等.2种紫花苜蓿幼苗生长对光照度的可塑性响应[J].江苏农业科学,2015,43(12):263.
　Zhang Lihui,et al.Plasticity response of seedling growth of two Medicago sativa species to light intensity[J].Jiangsu Agricultural Sciences,2015,43(07):263.
[8]刘胜洪,周玲艳,杨妙贤,等.60Co-γ射线诱变紫花苜蓿WL525HQ的SSR研究[J].江苏农业科学,2015,43(07):238.
　Liu Shenghong,et al..Study on 60Co-γ radiation inducing Medicago sativa based on SSR[J].Jiangsu Agricultural Sciences,2015,43(07):238.
[9]吴晓卫,付瑞敏,郭彦钊,等.耐盐碱微生物复合菌剂的选育、复配及其 对盐碱地的改良效果[J].江苏农业科学,2015,43(06):346.
　Wu Xiaowei,et al.Breeding and combination of saline-alkaline-tolerant microbes and their ameliorative effect on saline soils[J].Jiangsu Agricultural Sciences,2015,43(07):346.
[10]李玉波,许清涛,高标,等.脱硫石膏改良盐碱地对紫花苜蓿生长的影响[J].江苏农业科学,2015,43(03):188.
　Li Yubo,et al.Effect of saline-alkali land improved by desulfurization gypsum on growth of Medicago sativa[J].Jiangsu Agricultural Sciences,2015,43(07):188.

备注/Memo

备注/Memo:
收稿日期：2016-02-15
基金项目：国家自然科学基金(编号：31070224)。
作者简介：武慧(1986—)，女，吉林长春人，硕士，助理实验师，从事植物分子生物学方向研究。E-mail： sunflower329@163.com。
通信作者：周晓馥，博士，教授，博士生导师，从事植物基因工程方向研究。E-mail： zhouxiaofu@jlnu.edu.cn。
更新日期/Last Update: 2017-04-05