|本期目录/Table of Contents|

[1]黄诚梅,杨翠芳,魏源文,等.甘蔗蔗糖磷酸合成酶SPSⅢ基因表达分析[J].江苏农业科学,2018,46(02):17-20.
　Huang Chengmei,et al.Expression analysis of sucrose-phosphate synthase SPS Ⅲ gene in sugarcane[J].Jiangsu Agricultural Sciences,2018,46(02):17-20.
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甘蔗蔗糖磷酸合成酶SPSⅢ基因表达分析(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第46卷
期数:
2018年02期
页码:
17-20
栏目:
生物技术
出版日期:
2018-01-20

文章信息/Info

Title:
Expression analysis of sucrose-phosphate synthase SPS Ⅲ gene in sugarcane
作者:
黄诚梅1;  杨翠芳1; 2;  魏源文1;  邓智年2;  吴凯朝2;  曹辉庆1;  李杨瑞1; 2
1.广西作物遗传改良生物技术重点开放实验室，广西南宁 530007；
2.中国农业科学院甘蔗研究中心/农业部广西甘蔗生物技术与遗传改良重点实验室，广西南宁 530007
Author(s):
Huang Chengmei; et al
关键词:
甘蔗; 蔗糖磷酸合成酶基因SPSⅢ; 基因表达; 半定量RT-PCR; 糖分代谢
Keywords:
-
分类号:
S566.101
DOI:
-
文献标志码:
A
摘要:
为探讨蔗糖磷酸合成酶(sucrose phosphate synthase，简称SPS)对甘蔗糖分代谢的重要影响，以4个甘蔗品种桂糖28号(GT28)、果蔗Badila、新台糖20号(ROC20)、新台糖22号的+1、0叶(最高可见肥厚带的第1张完全展开叶为+1叶，自下而上位于+1叶之上的为0叶)、幼嫩叶鞘、幼茎等部位为材料，采用半定量RT-PCR技术分析甘蔗蔗糖磷酸合成酶基因(简称SPSⅢ)在工艺成熟期不同部位中的表达情况。结果表明，在工艺成熟期间，SPSⅢ在4个甘蔗基因型的+1、0叶、幼嫩叶鞘和幼茎中均有表达，表达量因基因型及测定部位与时期不同而不同；在工艺成熟初期，以GT28的4个部位较其他3个品种的低，而在果蔗Badila的4个部位中表达较稳定，在ROC20中以嫩叶鞘表达最高，而在ROC22中以幼茎表达最高，在工艺成熟中期，SPSⅢ在4个甘蔗基因型中的+1、0叶、幼嫩叶鞘和幼茎中的表达较初期没有明显差异；在工艺成熟后期，SPSⅢ在4个甘蔗基因型中的+1、0叶、幼嫩叶鞘和幼茎中的表达较前2个时期迅速提高，均达到了较高的表达水平。
Abstract:
-

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备注/Memo

备注/Memo:
收稿日期：2016-08-09
基金项目：“十二五”农村领域国家科技计划课题(编号：2013AA102604-1)；国家自然科学基金青年科学基金(编号：31400281)；广西自然科学基金(编号：2013GXNSFAA019077、2013GXNSFAA019088)；广西重点实验室建设项目(编号：12-071-09)；广西农业科学院团队项目(编号：桂农科2015YT96)；广西农业科学院科技发展基金(编号：桂农科2015JM24)。
作者简介：黄诚梅(1977—)，女，广西防城港人，博士，副研究员，主要从事甘蔗栽培与分子生物学研究。Tel：(0771)3279432；E-mail：huangchengmei@gxaas.net。
通信作者：李杨瑞，博士，教授，主要从事甘蔗研究。E-mail：liyr@gxaas.net。
更新日期/Last Update: 2018-01-20