|本期目录/Table of Contents|

[1]訾慧,白洪志,王惠,等.利用农业废弃物对青霉菌进行固态发酵生产纤维素酶[J].江苏农业科学,2018,46(05):285-289.
 Zi Hui,et al.Production of cellulase by solid state fermentation of Penicillium strain ZH1 using agricultural waste[J].Jiangsu Agricultural Sciences,2018,46(05):285-289.
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利用农业废弃物对青霉菌进行固态发酵生产纤维素酶(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第46卷
期数:
2018年05期
页码:
285-289
栏目:
资源与环境
出版日期:
2018-03-05

文章信息/Info

Title:
Production of cellulase by solid state fermentation of Penicillium strain ZH1 using agricultural waste
作者:
訾慧1 白洪志1 王惠2 韩晓日1 刘宁1 黄玉茜1 韩梅1 杨劲峰1
1.沈阳农业大学土地与环境学院,辽宁沈阳 110866; 2.沈阳农业大学生物科技学院,辽宁沈阳 110866
Author(s):
Zi Huiet al
关键词:
鉴定固体发酵纤维素酶青霉菌农业废弃物
Keywords:
-
分类号:
S188+.4
DOI:
-
文献标志码:
A
摘要:
筛选出1株产纤维素酶菌株并进行鉴定,为微生物肥料生产筛选菌种资源。采用刚果红染色、综纤维素、滤纸、微晶纤维素培养基等方法,从土壤中分离出1株产纤维素酶菌株ZH1,经形态学分析、18S rDNA分子进化树分析,鉴定菌株为赭绿青霉(Penicillium ochrochloron);采用单因素试验对产纤维素酶的青霉菌菌株进行固体发酵条件优化。结果表明,当温度为30 ℃、发酵液初始pH值为4、料水体积比为1.0 ∶3.0、接种量为0.5 mL及氮源为花生饼粉时,滤纸酶(FPase)在发酵3 d时有最大的产酶活性;通过优化试验,赭绿青霉达到了较高的产纤维素酶能力,为纤维素酶进一步工业化生产奠定了一定的基础。
Abstract:
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备注/Memo

备注/Memo:
收稿日期:2016-09-28
基金项目:国家科技支撑计划(编号:2013BAD08B04);国家现代农业产业技术体系专项(编号:CARS-14土壤肥料)。
作者简介:訾慧(1990—),女,吉林辽源人,硕士,主要从事纤维素降解菌的研究。E-mail:1187250716@qq.com。
通信作者:白洪志,博士,副教授,主要从事土壤微生物、植物营养等研究。E-mail:baihongzhi2003@sina.com。
更新日期/Last Update: 2018-03-05