|本期目录/Table of Contents|

[1]孙文斌,黄小京,冯蓓蓓,等.地枫皮内生真菌DFP-G-7的分离鉴定、生物学特性及抑菌活性[J].江苏农业科学,2018,46(20):97-100.
 Sun Wenbin,et al.Isolation, identification,biological characteristics and antimicrobial activity of entophytic fungus DFP-G-7 from Illicium difengpi[J].Jiangsu Agricultural Sciences,2018,46(20):97-100.
点击复制

地枫皮内生真菌DFP-G-7的分离鉴定、生物学
特性及抑菌活性
(PDF)
分享到:

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第46卷
期数:
2018年第20期
页码:
97-100
栏目:
植物保护
出版日期:
2018-10-20

文章信息/Info

Title:
Isolation, identification,biological characteristics and antimicrobial activity of entophytic fungus DFP-G-7 from Illicium difengpi
作者:
孙文斌 黄小京 冯蓓蓓 汤夏安 骆海玉 邓志勇 邓业成
珍稀濒危动植物生态与环境保护省部共建教育部重点实验室/广西师范大学生命科学学院,广西桂林 541006
Author(s):
Sun Wenbinet al
关键词:
地枫皮内生真菌分离鉴定生物学特性
Keywords:
-
分类号:
S182
DOI:
-
文献标志码:
A
摘要:
采用组织块分离法从地枫皮根中分离得到内生真菌DFP-G-7,采用形态学和分子生物学技术确定其种属关系,采用平板对峙法测定其抗菌活性,并探讨了主要营养因子和环境因子对其菌落生长的影响。结果表明:通过形态学和分子生物学技术鉴定内生真菌DFP-G-7为镰刀菌属真菌Fusarium nematophilum。6种不同碳源和6种不同氮源对内生真菌DFP-G-7菌落生长影响不大,说明其对碳源和氮源要求不严格。温度和pH值对内生真菌DFP-G-7菌落生长影响较大,在温度为25 ℃、培养基pH值为7时菌落生长最好。拮抗试验表明DFP-G-7对茶轮斑病菌、甘蓝黑斑病菌、甘蔗凤梨病菌、贡柑链格孢菌、玉米大斑病菌、辣椒炭疽病菌、水稻胡麻叶斑病菌和烟草黑胫病菌均有一定的抑制作用,抑菌率分别为50.64%、44.28%、27.54%、29.84%、32.54%、45.78%、33.14%和11.45%。可见从壮药地枫皮根中分离出的内生真菌DFP-G-7对植物病原真菌具有潜在的生防价值。
Abstract:
-

参考文献/References:

[1]高克祥. 内生真菌Chaetomium spirale,Stagonospora sp. 与病原菌、寄主植物相互作用的机制[D]. 杨凌:西北农林科技大学,2005:67.
[2]郭良栋. 内生真菌研究进展[J]. 菌物系统,2001,20(1):148-152.
[3]Malinowski D P,Belesky D P. Endophyte infection enhances the ability of tall fescue to utilize sparingly available phosphorus[J]. Journal of Plant Nutrition,1999(22):835-853.
[4]简在友,周修任,孟丽. 太行山野生红豆杉菌根菌的生态效应[J]. 土壤通报,2009,40(3):568-570.
[5]杨松,李春杰,黄玺. 被内生真菌侵染的禾草提取液对真菌的抑制作用[J]. 菌物学报,2010,29(2):234-240.
[6]Paivi T L,Marjo H,Shahid A S. Endophytic fungus decreases plant virus infections in meadow ryegrass (Lolium pratense)[J]. Biol Lett,2006(2):620-623.
[7]Lewis G C. Effects of biotic and abiotic stress on the growth of three genotypes of Lolium perenne with and without infection by the fungalendophyte Neotyphodium lolii[J]. Annals of Applied Biology,2004,144:53-63.
[8]Marks S,Clay K. Physiological responses of Festuca arundinacea to fungal endophyte infection[J]. New Phytologist,1996,133:727-733.
[9]Sutherland B L,Hume D E,Tupper B A. Allelopathic effects of endophyte-infected perennial ryegrass extracts on white clover seedlings[J]. New Zeal J Agr Res,1999,42:19-26.
[10]张本能. 中药地枫皮原植物的研究[J]. 植物研究通讯,1977(3):9-11.
[11]傅立国. 中国植物红皮书——稀有濒危植物(第一册)[M]. 北京:科学出版社,1992:62.
[12]国家药典委员会.中华人民共和国药典[M]. 北京:中国医药科技出版社,1995:98.
[13]Morton D T,Stroube N H. Antagonistic and stimulatory effects of microorganisms upon Sclerotium rolfsii[J]. Phytopathology,1955,45:419-420.
[14]Tayung K,Barik B P,Jha D K,et al. Identification andcharacterization of antimicrobial metabolite from anendophytic fungus,Fusarium solani isolated from bark of Himalayan yew[J]. Mycosphere,2011,2(3):203-213.
[15]Barik B P,Tayung K,Jagadev P N,et al. Phylogenetic placement of an endophytic fungus Fusarium oxysporum isolated from Acorus calamus rhizomes with antimicrobial activity[J]. EJBS,2010,2(1):8-16.

相似文献/References:

[1]高强,吴振莹,方玲,等.青檀内生真菌球毛壳的抗氧化活性[J].江苏农业科学,2013,41(05):293.
 Gao Qiang,et al.Antioxidant activity of endophytic fungi [WTBX]Chaetomium globosum[WTBZ] in [WTBX] Pteroceltis tatarinowii[WTBZ][J].Jiangsu Agricultural Sciences,2013,41(20):293.
[2]赵赟鑫,张欢,邓百万,等.红豆杉内生真菌产紫杉醇的培养基优化[J].江苏农业科学,2014,42(11):389.
 Zhao Yunxin,et al(89).Optimization of medium for Taxus chinensis endophytic fungi to produce taxol[J].Jiangsu Agricultural Sciences,2014,42(20):389.
[3]张波,梁雪飞,陈晏,等.内生真菌孔球孢霉对茅苍术的生长及挥发油主组分的影响[J].江苏农业科学,2013,41(06):204.
 Zhang Bo,et al.Effects of endophytic Gilmaniella sp. on growth and essential oil main components of Atractylodes lancea[J].Jiangsu Agricultural Sciences,2013,41(20):204.
[4]李丽,翟梅枝,杨惠,等.核桃叶部内生真菌发酵产物抑菌活性及GC-MS分析[J].江苏农业科学,2014,42(10):292.
 Li Li,et al.Antifungal activities and GC-MS analysis of endophytic fungi fermentation products in walnut leaves[J].Jiangsu Agricultural Sciences,2014,42(20):292.
[5]刘兆迪,解修超,陈文强,等.三尖杉属植物内生真菌及次生代谢产物活性研究进展[J].江苏农业科学,2013,41(08):11.
 Liu Zhaodi,et al.Research progress of Cephalotaxus plants endophytic fungi and their secondary metabolites activity[J].Jiangsu Agricultural Sciences,2013,41(20):11.
[6]郭建伟,郭娟,刘艳红,等.草果果腐病拮抗内生菌的筛选与初步鉴定[J].江苏农业科学,2014,42(08):116.
 Guo Jianwei,et al.Screening and primarily identification of antagonistic endophytic bacteria of Amomum tsao-ko fruit rot disease[J].Jiangsu Agricultural Sciences,2014,42(20):116.
[7]于淼,杨梅,张连茹.南方红豆杉内生真菌的初步研究[J].江苏农业科学,2013,41(09):332.
 Yu Miao,et al.Primary study on endophytic fungi from Taxus mairei[J].Jiangsu Agricultural Sciences,2013,41(20):332.
[8]詹寿发,樊有赋,甘金莲,等.1株山药内生真菌的鉴定及解钾活性[J].江苏农业科学,2013,41(10):320.
 Zhan Shoufa,et al.Identification and potassium releasing activities of a strain of endophytic fungal from Dioscorea opposita Thunb.[J].Jiangsu Agricultural Sciences,2013,41(20):320.
[9]杨建香,邱声祥,佘志刚,等.南海红树林内生真菌GX-5b代谢产物研究[J].江苏农业科学,2013,41(10):330.
 Yang Jianxiang,et al.Study on metabolites of mangrove endophytic fungus GX-5b from the South China Sea[J].Jiangsu Agricultural Sciences,2013,41(20):330.
[10]王立博,张婷,王敬力,等.油茶内生真菌DNA提取及SRAP反应体系的建立[J].江苏农业科学,2013,41(12):37.
 Wang Libo,et al.Extraction of DNA from Camellia oleifera endophytic fungi and establishment of SRAP reaction system[J].Jiangsu Agricultural Sciences,2013,41(20):37.
[11]冯蓓蓓,邓业成,卢丹丹,等.广西地不容和地枫皮内生真菌对罗汉果土传病原真菌的抑制活性[J].江苏农业科学,2023,51(1):123.
 Feng Beibei??et al.Study on inhibitory activity of endophytic fungi from Stephania kwangsiensis and Illicium difengpi against pathogenic fungi ofSiraitia grosvenorii[J].Jiangsu Agricultural Sciences,2023,51(20):123.

备注/Memo

备注/Memo:
收稿日期:2017-09-20
基金项目:广西自然科学基金(编号:2016GXNSFAA380207);广西研究生教育创新计划(编号:XYCSZ2017071)。
作者简介:孙文斌(1992—),男,吉林磐石人,硕士研究生,主要从事植物资源与利用研究。E-mail:sunwenbin1959@163.com。
通信作者:邓业成,博士,教授,主要从事天然产物与植物保护研究。E-mail:dyecheng@163.com。
更新日期/Last Update: 2018-10-20